Abstract:The use of autologous cells harvested and subsequently transplanted in an intraoperative environment constitutes a new approach to promote regeneration. Usually cells are isolated by selection methods such as fluorescence- or magnetic- activated cell sorting with residual binding of the antibodies or beads. Thus, cell-based therapies would benefit from the development of new devices for cell isolation that minimally manipulate the target cell population. In the clinic, 5 to 10 percent of fractures do not heal … Show more
“…This method suggests that young MSCs have great potential to differentiate into osteoblasts and adipocytes 112 . Similarly, CD31 + cells were collected from a mixture with CD31 − 293FT cells or peripheral blood mononuclear cells by a specific aptamer for CD31, and their potential for angiogenic and osteogenic behavior was confirmed in vitro 113 , 114 . An efficient device similar to double-sided tape was used to interfere with circulating tumor cells (CTCs) using ligand magnetic nanoparticles (MNPs).…”
Aptamers are single-stranded nucleic acids (DNA, short RNA, or other artificial molecules) produced by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology, which can be tightly and specifically combined with desired targets. As a comparable alternative to antibodies, aptamers have many advantages over traditional antibodies such as a strong chemical stability and rapid bulk production. In addition, aptamers can bind targets in various ways, and are not limited like the antigen–antibody combination. Studies have shown that aptamers have tremendous potential to diagnose and treat clinical diseases. However, only a few aptamer-based drugs have been used because of limitations of the aptamers and SELEX technology. To promote the development and applications of aptamers, we present a review of the methods optimizing the SELEX technology and modifying aptamers to boost the selection success rate and improve aptamer characteristics. In addition, we review the application of aptamers to treat bone diseases.
“…This method suggests that young MSCs have great potential to differentiate into osteoblasts and adipocytes 112 . Similarly, CD31 + cells were collected from a mixture with CD31 − 293FT cells or peripheral blood mononuclear cells by a specific aptamer for CD31, and their potential for angiogenic and osteogenic behavior was confirmed in vitro 113 , 114 . An efficient device similar to double-sided tape was used to interfere with circulating tumor cells (CTCs) using ligand magnetic nanoparticles (MNPs).…”
Aptamers are single-stranded nucleic acids (DNA, short RNA, or other artificial molecules) produced by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology, which can be tightly and specifically combined with desired targets. As a comparable alternative to antibodies, aptamers have many advantages over traditional antibodies such as a strong chemical stability and rapid bulk production. In addition, aptamers can bind targets in various ways, and are not limited like the antigen–antibody combination. Studies have shown that aptamers have tremendous potential to diagnose and treat clinical diseases. However, only a few aptamer-based drugs have been used because of limitations of the aptamers and SELEX technology. To promote the development and applications of aptamers, we present a review of the methods optimizing the SELEX technology and modifying aptamers to boost the selection success rate and improve aptamer characteristics. In addition, we review the application of aptamers to treat bone diseases.
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