Biophysical differentiation of susceptibility and chemical differences in<i>Staphylococcus aureus</i>

Hilton, Shannon Huey; Crowther, Claire V.; McLaren, A. C.; Smithers, Jared P; Hayes, Mark A.

doi:10.1039/c9an01449g

Cited by 11 publications

(13 citation statements)

References 58 publications

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“…Previous DC-iDEP work successfully distinguished closely related strains of microbes (Jones et al, 2014(Jones et al, , 2015Crowther et al, 2019;Hilton, 2019;Hilton et al, 2020). The connectivity between biophysical properties and current notions of "relatedness" of two strains is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The biophysical properties relate in a non-linear fashion with traditional cataloging systems [genetics, transcriptome, proteomics, molecular recognition (immune-, selex), and/or metabolic assessments]. In the author's laboratory, all previously attempted paired biophysical differentiation were successful and include: Staphylococcus epidermidis (gentamicin resistant/susceptible) (Jones et al, 2015), various strains of Escherichia coli (Jones et al, 2014), Listeria monocytogenes strains (Crowther et al, 2019), Staphylococcus aureus (methicillin resistance/susceptible) (Hilton et al, 2020), and Klebsiella pneumoniae (Hilton, 2019). Within the species Salmonella, some interesting future works would include the differentiation paired serotypes Salmonella typhimurium compared to Salmonella typimurium monophasic variant (Salmonella 1,4,[5],12:i:-) and Salmonella Indiana (O4,12;z;1,7) compared to Salmonella Loubomo (O4,12;z;1,6).…”

Section: Discussionmentioning

confidence: 99%

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Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Liu

Hayes

2020

Front. Microbiol.

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Salmonella is an important pathogen and is a worldwide threat to food safety and public health. Surveillance of serotypes and fundamental biological and biochemical studies are supported by a wide variety of established and emerging bioanalytical techniques. These include classic serotyping based on the Kauffmann-White nomenclature and the emerging whole genome sequencing strategy. Another emerging strategy is native whole cell biophysical characterization which has yet to be applied to Salmonella. However, this technique has been shown to provide high resolution differentiation of serotypes with several other paired strains of other microbes and pathogens. To demonstrate that biophysical characterization might be useful for Salmonella serotyping, the closely related strains sv. Cubana and sv. Poona were chosen for study. These two serovars were subjected to biophysical measurements on a dielectrophoresisbased microfluidic device that generated full differentiation of the unlabeled and native cells. They were differentiated by the ratio of electrophoretic (EP) to dielectrophoretic (DEP) mobilities. This differentiation factor is 2.7 ± 0.3 × 10 10 V/m 2 for sv. Cubana, versus 2.2 ± 0.3 × 10 10 V/m 2 for sv. Poona. This work shows for the first time the differentiation, concentration, and characterization of the Salmonella serotypes by exploiting their biophysical properties. It may lead to a less expensive and more decentralized new tool and method for microbiologists, complimenting and working in parallel with other characterization methods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Liu

Hayes

2020

Front. Microbiol.

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“…Choopara et al developed a fluorometric cellulose paper-based LAMP system for mecA detection in positive blood cultures [ 145 ]. Hilton et al used dielectrophoresis to identify possible changes (to be used as a marker or for developing rapid AST tests) in biophysical properties between one MSSA and one MRSA [ 146 ]. Schulz and coauthors developed a POC system that can detect MRSA, MSSA and mecA -positive CONS in single cells directly from nasal samples [ 147 ].…”

Section: Emerging Methods For Antimicrobial Resistance Detection In S Aureusmentioning

confidence: 99%

Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

Sanchini¹

2022

Diagnostics

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Staphylococcus aureus is an opportunistic pathogen responsible for a wide range of infections in humans, such as skin and soft tissue infections, pneumonia, food poisoning or sepsis. Historically, S. aureus was able to rapidly adapt to anti-staphylococcal antibiotics and become resistant to several classes of antibiotics. Today, methicillin-resistant S. aureus (MRSA) is a multidrug-resistant pathogen and is one of the most common bacteria responsible for hospital-acquired infections and outbreaks, in community settings as well. The rapid and accurate diagnosis of antimicrobial resistance in S. aureus is crucial to the early initiation of directed antibiotic therapy and to improve clinical outcomes for patients. In this narrative review, I provide an overview of recent phenotypic and molecular diagnostic methods for antimicrobial resistance detection in S. aureus, with a particular focus on MRSA detection. I consider methods for resistance detection in both clinical samples and isolated S. aureus cultures, along with a brief discussion of the advantages and the challenges of implementing such methods in routine diagnostics.

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“…Direct current insulator-based dielectrophoresis (DC-iDEP) provides a valuable tool for separating subpopulations of bioparticles with high resolution including viruses, bacteria, organelles, and proteins [13][14][15][16] . This approach offers a wide dynamic range, as it has been used for separations ranging from neural progenitors and stem cells 17 to resistant versus susceptible strains of cellular pathogens 15,18 . In DC-iDEP, subtle biophysical differences can be distinguished by the differences in dielectrophoretic (DEP) and electrokinetic (EK) forces that result from a rich set of distinguishing factors associated with the radius, zeta potential, permittivity, interfacial polarizability and conductivity of the particles, to name but a few [18][19][20][21][22] .…”

Section: Introductionmentioning

confidence: 99%

“…This approach offers a wide dynamic range, as it has been used for separations ranging from neural progenitors and stem cells 17 to resistant versus susceptible strains of cellular pathogens 15,18 . In DC-iDEP, subtle biophysical differences can be distinguished by the differences in dielectrophoretic (DEP) and electrokinetic (EK) forces that result from a rich set of distinguishing factors associated with the radius, zeta potential, permittivity, interfacial polarizability and conductivity of the particles, to name but a few [18][19][20][21][22] . DC-iDEP is well-suited for assaying subpopulations of organelles, where differentiating factors are not known, because it is unbiased compared to approaches that rely on defined biomarkers.…”

Section: Introductionmentioning

confidence: 99%

iDEP-assisted isolation of insulin secretory vesicles

Barekatain

Liu

Wang

et al. 2021

Preprint

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Organelle heterogeneity and inter-organelle associations within a single cell contribute to the limited sensitivity of current organelle separation techniques, thus hindering organelle subpopulation characterization. Here we use direct current insulator-based dielectrophoresis (DC-iDEP) as an unbiased separation method and demonstrate its capability by identifying distinct distribution patterns of insulin vesicles from pancreatic β-cells. A multiple voltage DC-iDEP strategy with increased range and sensitivity has been applied, and a differentiation factor (ratio of electrokinetic to dielectrophoretic mobility) has been used to characterize features of insulin vesicle distribution patterns. We observed a significant difference in the distribution pattern of insulin vesicles isolated from glucose-stimulated cells relative to unstimulated cells, in accordance with functional maturation of vesicles upon glucose stimulation, and interpret this to be indicative of high-resolution separation of vesicle subpopulation. DC-iDEP provides a path for future characterization of subtle biochemical differences of organelle subpopulations within any biological system.

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Biophysical differentiation of susceptibility and chemical differences inStaphylococcus aureus

Abstract: Dielectrophoresis and electrophoresis are used to differentiate native cells for methicillin resistant (MRSA) and susceptible Staphylococcus aureus strains.

Cited by 11 publications

References 58 publications

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

iDEP-assisted isolation of insulin secretory vesicles

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