Biophysical characterization of mycobacterial model membranes and their interaction with rifabutin: Towards lipid-guided drug screening in tuberculosis

Dadhich, Ruchika; Singh, Abhishek Kumar; Menon, Anjana P.; Mishra, Manjari; Athul, C.D.; Kapoor, Shobhna

doi:10.1016/j.bbamem.2019.04.004

Cited by 15 publications

(20 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Complementary to image‐based viscosity profiling are spectroscopy‐based methods, which offer a quick screening of membrane viscosity using lipid probes and can easily be adapted to a high‐throughput scale (Figure 4B). 156–158 The lipid probe diphenylhexatriene (DPH) intercalates near the acyl chains, with its charged counterpart trimethylamino (TMA)‐DPH being 3–4 Å shallower than DPH 159 . Thus, DPH provides information on the hydrophobic acyl region and TMA‐DPH on the interfacial head group region of the lipid membrane and can be used for the in‐depth understanding of the mechanisms linking chemoresistance to microscopic viscosity changes.…”

Section: The Road Ahead: How To Harness Lipid Membrane Properties For...mentioning

confidence: 99%

“…[156][157][158] The lipid probe diphenylhexatriene (DPH) intercalates near the acyl chains, with its charged counterpart trimethylamino (TMA)-DPH being 3-4 Å shallower than DPH. 159 Thus, DPH provides information on the hydrophobic acyl region and TMA-DPH on the interfacial head group region of the lipid membrane and can be used for the in-depth understanding of the mechanisms linking chemoresistance to microscopic viscosity changes.…”

Section: The Road Ahead: How To Harness Lipid Membrane Properties For...mentioning

confidence: 99%

See 1 more Smart Citation

Lipidomic landscape in cancer: Actionable insights for membrane‐based therapy and diagnoses

Agarwala

Aneja

Kapoor

2021

Medicinal Research Reviews

Self Cite

View full text Add to dashboard Cite

Cancer cells display altered cellular lipid metabolism, including disruption in endogenous lipid synthesis, storage, and exogenous uptake for membrane biogenesis and functions. Altered lipid metabolism and, consequently, lipid composition impacts cellular function by affecting membrane structure and properties, such as fluidity, rigidity, membrane dynamics, and lateral organization. Herein, we provide an overview of lipid membranes and how their properties affect cellular functions. We also detail how the rewiring of lipid metabolism impacts the lipidomic landscape of cancer cell membranes and influences the characteristics of cancer cells. Furthermore, we discuss how the altered cancer lipidome provides cues for developing lipid‐inspired innovative therapeutic and diagnostic strategies while improving our limited understanding of the role of lipids in cancer initiation and progression. We also present the arcade of membrane characterization techniques to cement their relevance in cancer diagnosis and monitoring of treatment response.

show abstract

Section: The Road Ahead: How To Harness Lipid Membrane Properties For...mentioning

confidence: 99%

Section: The Road Ahead: How To Harness Lipid Membrane Properties For...mentioning

confidence: 99%

Lipidomic landscape in cancer: Actionable insights for membrane‐based therapy and diagnoses

Agarwala

Aneja

Kapoor

2021

Medicinal Research Reviews

Self Cite

View full text Add to dashboard Cite

show abstract

“…Drug‐membrane interactions are becoming increasingly critical in drug discovery [4b,5] . Membrane‐drug interactions are lipid composition‐specific; head group and acyl chain structures, conformations, hydrophobicity, and stereochemistry affect drug passage [3b,6] …”

Section: Introductionmentioning

confidence: 99%

Lipid Clustering in Mycobacterial Cell Envelope Layers Governs Spatially Resolved Solvation Dynamics

Adhyapak

Dong

Dasgupta

et al. 2022

Chemistry An Asian Journal

Self Cite

View full text Add to dashboard Cite

The mycobacterial cell envelope acts as a multilayered barrier to drugs. However, the role of lipid composition in the properties of different mycobacterial membranes, otherwise dictating their interactions with drugs, is poorly understood. In this study, we found that hydration states, solvation relaxation kinetics, rotational lipid mobility, and lateral lipid diffusion differed between inner and outer mycobacterial membranes. Molecular modeling showed that lipid clustering patterns governed membrane dynamics in the different layers of the cell envelope. By regulating membrane properties, lipid composition and structure modulated water abundance and interactions with lipid head groups. These findings can help deepen our understanding of the physical chemistry underlying membrane structure and function, as well as the interaction of mycobacterial membranes with drugs and host membranes.

show abstract

“…Aligning with the approach of lipid-guided drug screening in tuberculosis, Dadhich et al . developed membrane scaffolds specific to mycobacterial OM and demonstrated them as selective and novel platforms for investigating anti-tubercular drug interactions (Dadhich et al 2019 ). Designed membrane scaffolds such as TDM:DAG:DPPG (9.1:18.2:72.7 mol %), TDM:DAG (33.3:66.6 mol %) and TDM were characterized for their interaction with antituberculotic drug, rifabutin.…”

Section: Lipids In Bacterial Infectionsmentioning

confidence: 99%

Various Facets of Pathogenic Lipids in Infectious Diseases: Exploring Virulent Lipid-Host Interactome and Their Druggability

Dadhich

Kapoor

2020

J Membrane Biol

Self Cite

View full text Add to dashboard Cite

Lipids form an integral, structural, and functional part of all life forms. They play a significant role in various cellular processes such as membrane fusion, fission, endocytosis, protein trafficking, and protein functions. Interestingly, recent studies have revealed their more impactful and critical involvement in infectious diseases, starting with the manipulation of the host membrane to facilitate pathogenic entry. Thereafter, pathogens recruit specific host lipids for the maintenance of favorable intracellular niche to augment their survival and proliferation. In this review, we showcase the lipid-mediated host pathogen interplay in context of life-threatening viral and bacterial diseases including the recent SARS-CoV-2 infection. We evaluate the emergent lipid-centric approaches adopted by these pathogens, while delineating the alterations in the composition and organization of the cell membrane within the host, as well as the pathogen. Lastly, crucial nexus points in their interaction landscape for therapeutic interventions are identified.

show abstract

Biophysical characterization of mycobacterial model membranes and their interaction with rifabutin: Towards lipid-guided drug screening in tuberculosis

Cited by 15 publications

References 57 publications

Lipidomic landscape in cancer: Actionable insights for membrane‐based therapy and diagnoses

Lipidomic landscape in cancer: Actionable insights for membrane‐based therapy and diagnoses

Lipid Clustering in Mycobacterial Cell Envelope Layers Governs Spatially Resolved Solvation Dynamics

Various Facets of Pathogenic Lipids in Infectious Diseases: Exploring Virulent Lipid-Host Interactome and Their Druggability

Contact Info

Product

Resources

About