“…Although a full biogenesis mechanism has yet to be revealed, OIMVs may form as a result of autolysin activity that transiently breaks down peptidoglycan to allow OIMV release (Kadurugamuwa & Beveridge, 1995; Pérez‐Cruz et al , 2013, 2015; Clarke, 2018). Naturally produced OIMVs have been observed in many different species including Shewanella vesiculosa , Neisseria gonorrhoeae , P. aeruginosa , Acinetobacter baumannii, V. shilonii , Pseudoalteromonas marina , Arhensia kielensis , and P. syringae , among others (Hagemann et al , 2014; Pérez‐Cruz et al , 2015; Li et al , 2016; preprint: Janda et al , 2021). In P. aeruginosa , vesicle production can also result from explosive cell lysis triggered by endolysins, yielding so‐called explosive OMVs (EOMVs) (Fig 3F) (Turnbull et al , 2016; Toyofuku et al , 2019).…”