Biophysical and biological characterisation of collagen/resilin-like protein composite fibres

Sanami, Mohammad; Shtein, Zvi; Sweeney, I; Sorushanova, Anna; Rivkin, Amit; Miraftab, Mohsen; Shoseyov, Oded; O'Dowd, Colm; Mullen, Anne Maria; Pandit, Abhay; Zeugolis, Dimitrios I.

doi:10.1088/1748-6041/10/6/065005

Cited by 16 publications

(14 citation statements)

References 40 publications

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“…In addition, RLP 24 -RGD 2 -MMP-HBD-based hybrid hydrogel showed successful encapsulation and viable 3D culture of hMSCs, and tuneable strain to break and resilience in the range of 68-173% and 90-98% (at 20% strain), respectively 72 . Subsequently, RLP-based hybrid hydrogel fibres, namely RLP-CF fibres, were fabricated by crosslinking elastic RLP and stiff collagen fibres (CF) using 4-arm PEG-ether tetrasuccinimidyl glutarate as crosslinker, which interacts with amine groups of both proteins 73 . The hybrid fibres showed improved biomechanical properties, and human adult fibroblast cell attachment and proliferation 73 .…”

Section: Mechanism Of Elasticity and Chemical Crosslinking Of Rlpsmentioning

confidence: 99%

“…Subsequently, RLP-based hybrid hydrogel fibres, namely RLP-CF fibres, were fabricated by crosslinking elastic RLP and stiff collagen fibres (CF) using 4-arm PEG-ether tetrasuccinimidyl glutarate as crosslinker, which interacts with amine groups of both proteins 73 . The hybrid fibres showed improved biomechanical properties, and human adult fibroblast cell attachment and proliferation 73 . In a separate study, the RLP-based hybrid hydrogel, namely RLP-SF was fabricated by ruthenium-mediated photocrosslinking of tyrosine residues between the resilin and silk fibroin molecules (i.e.…”

Section: Mechanism Of Elasticity and Chemical Crosslinking Of Rlpsmentioning

confidence: 99%

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Resilin-mimetics as a smart biomaterial platform for biomedical applications

Balu

Dutta

et al. 2021

Nat Commun

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Intrinsically disordered proteins have dramatically changed the structure–function paradigm of proteins in the 21st century. Resilin is a native elastic insect protein, which features intrinsically disordered structure, unusual multi-stimuli responsiveness and outstanding resilience. Advances in computational techniques, polypeptide synthesis methods and modular protein engineering routines have led to the development of novel resilin-like polypeptides (RLPs) including modular RLPs, expanding their applications in tissue engineering, drug delivery, bioimaging, biosensors, catalysis and bioelectronics. However, how the responsive behaviour of RLPs is encoded in the amino acid sequence level remains elusive. This review summarises the milestones of RLPs, and discusses the development of modular RLP-based biomaterials, their current applications, challenges and future perspectives. A perspective of future research is that sequence and responsiveness profiling of RLPs can provide a new platform for the design and development of new modular RLP-based biomaterials with programmable structure, properties and functions.

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Section: Mechanism Of Elasticity and Chemical Crosslinking Of Rlpsmentioning

confidence: 99%

Section: Mechanism Of Elasticity and Chemical Crosslinking Of Rlpsmentioning

confidence: 99%

Resilin-mimetics as a smart biomaterial platform for biomedical applications

Balu

Dutta

et al. 2021

Nat Commun

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“…Resilin is an arthropods protein with elastic and highly stretchable structure. Sanami, M., et al [27] investigated the fabrication of such composite. The scaffold was made through extrusion process of composite solution containing Collagen and Resilin at different concentration into polyethylene glycol buffer.…”

Section: Biological Composite Scaffoldsmentioning

confidence: 99%

Advances in Tendon and Ligament Tissue Engineering: Materials Perspective

Alshomer

Chaves

Kalaskar

2018

Journal of Materials

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Introduction. Tendons are specialised, heterogeneous connective tissues, which represent a significant healthcare challenge after injury. Primary surgical repair is the gold standard modality of care; however, it is highly dependent on the extent of injuries. Tissue engineering represents an alternative solution for good tissue integration and regeneration. In this review, we look at the advanced biomaterial composites employed to improve cellular growth while providing appropriate mechanical properties for tendon and ligament repair. Methodology. Comprehensive literature searches focused on advanced composite biomaterials for tendon and ligament tissue engineering. Studies were categorised depending on the application. Results. In the literature, a range of natural and/or synthetic materials have been combined to produce composite scaffolds tendon and ligament tissue engineering. In vitro and in vivo assessment demonstrate promising cellular integration with sufficient mechanical strength. The biological properties were improved with the addition of growth factors within the composite materials. Most in vivo studies were completed in smallscale animal models. Conclusions. Advanced composite materials represent a promising solution to the challenges associated with tendon and ligament tissue engineering. Nevertheless, these approaches still demonstrate limitations, including the necessity of larger-scale animal models to ease future clinical translation and comprehensive assessment of tissue response after implantation.

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“…Four exogenous cross-linkers frequently used in tissue engineering applications [13,[16][17][18]23,30,31] were used including glutaraldehyde (GTA) [16,17,32], genipin (GNP) [15,22,33], poly (ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) [12,17,30,31], and succinimidyl carboxymethyl ester (SCM-PEG-SCM) [29,34] (Fig. 2).…”

Section: Crosslinking Strategiesmentioning

confidence: 99%

Cross-linking of a biopolymer-peptide co-assembling system

et al. 2017

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The ability to guide molecular self-assembly at the nanoscale into complex macroscopic structures could enable the development of functional synthetic materials that exhibit properties of natural tissues such as hierarchy, adaptability, and self-healing. However, the stability and structural integrity of these kinds of materials remains a challenge for many practical applications. We have recently developed a dynamic biopolymer-peptide co-assembly system with the capacity to grow and undergo morphogenesis into complex shapes. Here we explored the potential of different synthetic (succinimidyl carboxymethyl ester [SCM-PEG-SCM], poly (ethylene glycol) ether tetrasuccinimidyl glutarate [4S-StarPEG] and glutaraldehyde) and natural (genipin) cross-linking agents to stabilize membranes made from these biopolymer-peptide co-assemblies. We investigated the cross-linking efficiency, resistance to enzymatic degradation, and mechanical properties of the different cross-linked membranes. We also compared their biocompatibility by assessing the metabolic activity and morphology of adipose-derived stem cells (ADSC) cultured on the different membranes. While all cross-linkers successfully stabilized the system under physiological conditions, membranes cross-linked with genipin exhibited better resistance in physiological environments, improved stability under enzymatic degradation, and a higher degree of in vitro cytocompatibility compared to the other cross-linking agents. The results demonstrated that genipin is an attractive candidate to provide functional structural stability to complex self-assembling structures for potential tissue engineering or in vitro model applications. Statement of SignificanceMolecular self-assembly is widely used for the fabrication of complex functional biomaterials to replace and/or repair any tissue or organ in the body. However, maintaining the stability and corresponding functionality of these kinds of materials in physiological conditions remains a challenge. Chemical cross-linking strategies (natural or synthetic) have been used in an effort to improve their structural integrity. Here we investigate key performance parameters of different cross-linking strategies for stabilising self-assembled materials with potential biomedical applications using a novel protein-peptide co-assembling membrane as proof-of-concept. From the different cross-linkers tested, the natural cross-linker genipin exhibited the best performance. This cross-linker successfully enhanced the mechanical properties of the system enabling the maintenance of the structure in physiological conditions without compromising its bioactivity and biocompatibility. Altogether, we provide a systematic characterization of cross-linking alternatives for self-assembling materials focused on biocompatibility and stability and demonstrate that genipin is a promising alternative for the cross-linking of such materials with a wide variety of potential applications such as in tissue engineering and drug delivery.

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Biophysical and biological characterisation of collagen/resilin-like protein composite fibres

Cited by 16 publications

References 40 publications

Resilin-mimetics as a smart biomaterial platform for biomedical applications

Resilin-mimetics as a smart biomaterial platform for biomedical applications

Advances in Tendon and Ligament Tissue Engineering: Materials Perspective

Cross-linking of a biopolymer-peptide co-assembling system

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