Biophysical and biochemical insights in the design of immunoassays

Calidonio, Josselyn Mata; Hamad‐Schifferli, Kimberly

doi:10.1016/j.bbagen.2022.130266

Cited by 5 publications

(3 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chemosensors 2023, 11, 66 8 of 18 7.4) with a concentration of 25 mM, where the sensor was kept for 5 min. Then, the sensor was further rinsed in 1.5 mL of deionized water to remove residual buffer solution and non-crosslinked omalizumab, and finally it was immersed in deionized water for impedance spectrum measurements.…”

Section: Impedance Spectroscopymentioning

confidence: 99%

See 1 more Smart Citation

Impedimetric Biosensor Coated with Zinc Oxide Nanorods Synthesized by a Modification of the Hydrothermal Method for Antibody Detection

et al. 2023

View full text Add to dashboard Cite

Impedimetric biosensors are used for detecting a wide range of analytes. The detection principle is a perspective for the development of new types of analytical devices for biomolecular diagnosis of diseases. Of particular interest are biosensors with very high sensitivities, capable of detecting trace amounts of biomarkers or drugs in biological fluids. Impedimetric biosensors possess a potential for increased sensitivity, since their electrodes can be modified with nanostructured materials, in particular zinc oxide. In this work, a miniature biosensor with an array of zinc oxide nanorods synthesized by the hydrothermal method has been created. Protein A was immobilized on the resulting structure, which was previously tested for binding to omalizumab by capillary electrophoresis. Using impedance spectroscopy, it was possible to detect the binding of omalizumab at concentrations down to 5 pg/mL. The resulting structures are suitable for creating reusable biosensor systems, since ZnO-coated electrodes are easily cleaned by photocatalytic decomposition of the bound molecules. The biosensor is promising for use in Point-of-Care systems designed for fast, multimodal detection of molecular markers of a wide range of diseases.

show abstract

Section: Impedance Spectroscopymentioning

confidence: 99%

“…Antibodies are either monoclonal or polyclonal. Monoclonal antibodies are produced by B cells that are clones of the same parent cell and are monovalent when they recognize the same antigen epitope [7]. This type of antibody is produced ex vivo in tissue culture.…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Biosensor Coated with Zinc Oxide Nanorods Synthesized by a Modification of the Hydrothermal Method for Antibody Detection

et al. 2023

View full text Add to dashboard Cite

show abstract

“…However, rapid diagnostic production possesses major bottlenecks that inhibit rapid response. With each new disease, an entirely new LFA must be developed for a corresponding target, 2 requiring production of specific antibodies. The process of developing new antibodies can take 1–2 years and cost hundreds of millions of dollars.…”

Section: Introductionmentioning

confidence: 99%