Bioorthogonal regulation of DNA circuits for smart intracellular microRNA imaging

Abstract

An on-site bioorthogonal regulated DNA circuit was developed by introducing an endogenous DNA repairing enzyme-mediated sequential activation strategy to achieve cancer cell-selective microRNA imaging with high anti-interference ability.

Stabilization of Gold Nanoparticles by Hairpin DNA and Implications for Label-Free Colorimetric Biosensors

“…48−50 For either interaction mode, DNA adsorption to the gold surface via strong base coordination. 26,51 For many applications such as hybridization chain reactions, 14 hairpins with a tail are used (Figure 1B) and the hairpins can interact with AuNPs via both the loop region and the tail. DNA plays an important role in increasing the colloidal stability of AuNPs (Figure 1C).…”

“…12 DNA hairpins can also kinetically trap a system, and this property has been used to develop hybridization chain reactions. 13,14 Hairpins have been used as sensing probes and immobilized on various substrates, such as glass, 15,16 gold, 17,18 magnetic particles, 19 and plastics 20,21 to construct biosensor platforms. 22−24 For some applications, one or both stems end with a tail overhang.…”

Monitoring and modulating a catalytic hybridization circuit for self-adaptive bioorthogonal DNA assembly

“…With different expressions of folate receptor (FR) on the cell membrane, human breast cancer (MCF-7) and normal MCF-10A cells were used as models to investigate the living-cell imaging. [36][37][38][39] Noteworthy, here the nanovesicles only contained random FAM-modified DNA to evaluate the FR-mediated endocytosis. As shown in Figure S11, FA-nanovesicles exhibited an enhanced fluorescence signal as compared to that of the unmodified nanovesicles after 3 h of incubation in MCF-7 cells.…”

“…With different expressions of the folate receptor (FR) on the cell membrane, human breast cancer (MCF-7) and normal MCF-10A cells were used as models to investigate the living-cell imaging. [36][37][38][39] Noteworthily, here the nanovesicles only contained random FAM-modied DNA to evaluate the FRmediated endocytosis. As shown in Fig.…”

Self-assembly of DNA nanogels with endogenous microRNA toehold self-regulating switches for targeted gene regulation therapy

“…Therefore, the preparation of miRNA responsive DNA nanohydrogels is a very promising design approach for the targeted regulation therapy of cancer. 22 However, DNA exhibits a strong electronegativity, and it is challenging for DNA to be endocytosed by cancer cells. 23,24 In addition, the DNA hydrogel bears low hydrophobic drug loading capacity, [25][26][27] which greatly restricts its synergistic application with first-line anticancer drugs.…”