Bioorthogonal Reaction-Mediated Enzymatic Elongation-Driven Dendritic Nanoassembly for Genome-Wide Analysis of 5-Hydroxymethyluracil in Breast Tissues

Jiang, Su; Cai, Yanbo; Zhang, Qian-yi; Liu, Qian; Wang, Zi-yue; Zhang, Chun-yang

doi:10.1021/acs.nanolett.3c03754

Cited by 3 publications

(1 citation statement)

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“…The reported methods for nucleic acid modification quantification include thin-layer chromatography (TLC) [ 10 ], liquid chromatography (LC) or capillary electrophoresis (CE) based on optical detection [ 27 , 28 , 29 ], gas chromatography–mass spectrometry (GC–MS) [ 30 ], liquid chromatography–mass spectrometry (LC–MS), surface-enhanced Raman scattering (SERS) spectroscopy [ 31 ], immunoassay, and biosensing-based methods [ 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. MS-based methods have been considered as the main analytical tool for nucleic acid modification quantification due to their wide applicability, excellent sensitivity, and wide linear range, providing comparative global compositional analysis of different biological samples.…”

Section: Qualitative and Quantitative Analysismentioning

confidence: 99%

Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery

Liu,

Dong,

Shen

et al. 2024

IJMS

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Nucleic acid modifications play important roles in biological activities and disease occurrences, and have been considered as cancer biomarkers. Due to the relatively low amount of nucleic acid modifications in biological samples, it is necessary to develop sensitive and reliable qualitative and quantitative methods to reveal the content of any modifications. In this review, the key processes affecting the qualitative and quantitative analyses are discussed, such as sample digestion, nucleoside extraction, chemical labeling, chromatographic separation, mass spectrometry detection, and data processing. The improvement of the detection sensitivity and specificity of analytical methods based on mass spectrometry makes it possible to study low-abundance modifications and their biological functions. Some typical nucleic acid modifications and their potential as biomarkers are displayed, and efforts to improve diagnostic accuracy are discussed. Future perspectives are raised for this research field.

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