BION-M 1: First continuous blood pressure monitoring in mice during a 30-day spaceflight

Andreev-Andrievskiy, Alexander; Popova, Anfisa; Lloret, Jean-Christophe; Aubry, P; Боровик, А.C.; Tsvirkun, Daria; Виноградова, О. Л.; Ilyin, Е.А.; Gauquelin‐Koch, Guillemette; Gharib, Claude; Custaud, Marc‐Antoine

doi:10.1016/j.lssr.2017.03.002

Cited by 8 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, mice have a negligible hydrostatic pressure gradient on Earth owing to their small body size and do not show the same shifts in fluid volume during hindlimb unloading as that found in rats 33 . Additionally, during 30 days of spaceflight on the Bion-M1 satellite, mean arterial blood pressure in the aortic arch of mice is not altered 34 , and arteries in the head and hindlimbs of mice flown on the Bion-M1 and Space Shuttle do not show any structural remodeling to indicate chronic changes in fluid pressure associated with a cephalad fluid shift 26,27,35 . Thus, an impairment of BRB integrity in the eyes of mice during spaceflight may not result in an increase in IOP due to the lack of a cephalad fluid shift, as might be reasonably expected to occur in astronauts.…”

Section: Discussionmentioning

confidence: 96%

Characterization of mouse ocular response to a 35-day spaceflight mission: Evidence of blood-retinal barrier disruption and ocular adaptations

Mao

Nishiyama

Byrum

et al. 2019

Sci Rep

View full text Add to dashboard Cite

The health risks associated with spaceflight-induced ocular structural and functional damage has become a recent concern for NASA. The goal of the present study was to characterize the effects of spaceflight and reentry to 1 g on the structure and integrity of the retina and blood-retinal barrier (BRB) in the eye. To investigate possible mechanisms, changes in protein expression profiles were examined in mouse ocular tissue after spaceflight. Ten week old male C57BL/6 mice were launched to the International Space Station (ISS) on Space-X 12 at the Kennedy Space Center (KSC) on August, 2017. After a 35-day mission, mice were returned to Earth alive. Within 38 +/− 4 hours of splashdown, mice were euthanized and ocular tissues were collected for analysis. Ground control (GC) and vivarium control mice were maintained on Earth in flight hardware or normal vivarium cages respectively. Repeated intraocular pressure (IOP) measurements were performed before the flight launch and re-measured before the mice were euthanized after splashdown. IOP was significantly lower in post-flight measurements compared to that of pre-flight (14.4–19.3 mmHg vs 16.3–20.3 mmHg) (p < 0.05) for the left eye. Flight group had significant apoptosis in the retina and retinal vascular endothelial cells compared to control groups (p < 0.05). Immunohistochemical analysis of the retina revealed that an increased expression of aquaporin-4 (AQP-4) in the flight mice compared to controls gave strong indication of disturbance of BRB integrity. There were also a significant increase in the expression of platelet endothelial cell adhesion molecule-1 (PECAM-1) and a decrease in the expression of the BRB-related tight junction protein, Zonula occludens-1 (ZO-1). Proteomic analysis showed that many key proteins and pathways responsible for cell death, cell cycle, immune response, mitochondrial function and metabolic stress were significantly altered in the flight mice compared to ground control animals. These data indicate a complex cellular response that may alter retina structure and BRB integrity following long-term spaceflight.

show abstract

Section: Discussionmentioning

confidence: 96%

Characterization of mouse ocular response to a 35-day spaceflight mission: Evidence of blood-retinal barrier disruption and ocular adaptations

Mao

Nishiyama

Byrum

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In centrifugation as well as during parabolic flights, the in vivo responses to angiogenic factors have not yet been investigated. Moreover, it has been shown that during the takeoff and landing of a space module (BION-M 1), hypergravity induces cardiovascular changes [89]. More experiments should be conducted to precise how these cardiovascular changes can modify the structure of the BBB and neurovascular unit functions.…”

Section: Discussionmentioning

confidence: 99%

Hypergravity Increases Blood–Brain Barrier Permeability to Fluorescent Dextran and Antisense Oligonucleotide in Mice

Dubayle

Vanden‐Bossche

Peixoto

et al. 2023

Cells

View full text Add to dashboard Cite

The earliest effect of spaceflight is an alteration in vestibular function due to microgravity. Hypergravity exposure induced by centrifugation is also able to provoke motion sickness. The blood–brain barrier (BBB) is the crucial interface between the vascular system and the brain to ensure efficient neuronal activity. We developed experimental protocols of hypergravity on C57Bl/6JRJ mice to induce motion sickness and reveal its effects on the BBB. Mice were centrifuged at 2× g for 24 h. Fluorescent dextrans with different sizes (40, 70 and 150 kDa) and fluorescent antisense oligonucleotides (AS) were injected into mice retro-orbitally. The presence of fluorescent molecules was revealed by epifluorescence and confocal microscopies in brain slices. Gene expression was evaluated by RT-qPCR from brain extracts. Only the 70 kDa dextran and AS were detected in the parenchyma of several brain regions, suggesting an alteration in the BBB. Moreover, Ctnnd1, Gja4 and Actn1 were upregulated, whereas Jup, Tjp2, Gja1, Actn2, Actn4, Cdh2 and Ocln genes were downregulated, specifically suggesting a dysregulation in the tight junctions of endothelial cells forming the BBB. Our results confirm the alteration in the BBB after a short period of hypergravity exposure.

show abstract

Mice display learning and behavioral deficits after a 30-day spaceflight on Bion-M1 satellite

Andreev-Andrievskiy

Dolgov

Alberts

et al. 2022

Behavioural Brain Research

View full text Add to dashboard Cite

BION-M 1: First continuous blood pressure monitoring in mice during a 30-day spaceflight

Cited by 8 publications

References 36 publications

Characterization of mouse ocular response to a 35-day spaceflight mission: Evidence of blood-retinal barrier disruption and ocular adaptations

Characterization of mouse ocular response to a 35-day spaceflight mission: Evidence of blood-retinal barrier disruption and ocular adaptations

Hypergravity Increases Blood–Brain Barrier Permeability to Fluorescent Dextran and Antisense Oligonucleotide in Mice

Mice display learning and behavioral deficits after a 30-day spaceflight on Bion-M1 satellite

Contact Info

Product

Resources

About