Biomphalaria embryotoxicity test (BET): 60 years of research crossing boundaries for developing standard protocols

Caixeta, Maxwell Batista; Araújo, Paula Sampaio; Pereira, Aryelle Canedo; Tallarico, Lenita de Freitas; Rocha, Thiago Lopes

doi:10.1016/j.scitotenv.2022.155211

Cited by 11 publications

(10 citation statements)

References 81 publications

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“…Authors described that due to embryos are surrounded by different membranes and substances they were protected against the pesticide. In concordance, low bioavailability and toxicity of silver nanoparticles ( Araújo et al, 2020 ) and ivermectin ( Katz et al, 2017 ) to B. glabrata embryos were associated with the protective role of the gelatinous membrane ( Caixeta et al, 2022 ). B. straminea snails exposed to water from Lugano Lake laid egg masses with morphological changes including eggs with abnormal forms such as non-round eggs, overlapping eggs and eggs with arrested development at different stages.…”

Section: Discussionmentioning

confidence: 94%

“…Biomphalaria spp. have been indicated as useful models for assessing chemical toxicity using early development stages mainly during the embryogenesis process ( Caixeta et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…Also, loss in the synchronicity of hatching and decrease in the hatching rate were observed. Hatching inhibition can be related to developmental delays, interactions with gelatinous membrane and enzyme activities inhibition ( Caixeta et al, 2022 ). Our results could indicate that one or a mix of compounds present in the water samples have high permeability, reached the embryos and caused negative effects on development and hatching.…”

Section: Discussionmentioning

confidence: 99%

“…Their slow mobility and difficulty to detoxify pollutants make them sensitive species for water contamination ( Gao et al, 2017 ). Generally, embryos and juveniles are more sensitive to pollutants than adult gastropods ( Leung et al, 2007 ; Khangarot and Das, 2010 ; Attia et al, 2017 ; Agrelo et al, 2019 ), so toxicity data using the most vulnerable life stage would offer protection for all developmental stages in the environment ( Caixeta et al, 2022 ). However, not all gastropods are useful for reproduction assays due to their low rate of reproduction and slow development and growth, such as Chilina spp.…”

Section: Introductionmentioning

confidence: 99%

“…Biomphalaria spp. (Gastropoda, Planorbidae) has many advantages that make it a suitable model system in reproduction assays such as high egg production, fast development and growth, short life cycles and transparent layer covering the embryos ( Cossi et al, 2020 ; Caixeta et al, 2022 ). These snails are simultaneous hermaphrodites, and are able to reproduce by both self-fertilization and cross-fertilization.…”

Section: Introductionmentioning

confidence: 99%

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Aquatic Contamination in Lugano Lake (Lugano Lake Ecological Reserve, Buenos Aires, Argentina) Cause Negative Effects on the Reproduction and Juvenile Survival of the Native Gastropod Biomphalaria straminea

et al. 2022

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Lugano Lake is located in an Ecological Reserve of Buenos Aires City. Biomonitoring of its water quality is essential due to its importance as a place for recreation and protection of native species. Biomphalaria straminea is a native hermaphrodite aquatic gastropod that inhabits different freshwater bodies of Argentina and was recently selected as a potential bioindicator. We propose this study as a first approach to assessing specific organisms’ use in biomonitoring of urban wild reserves, and the usefulness of reproduction assays. B. straminea survival, behavior, reproduction success and offspring survival after the exposure to water samples from Lugano Lake (L1, L2, and L3) were evaluated. Temperature, pH, conductivity and dissolved oxygen were registered in situ. Samples were transported to the laboratory and chemical analysis and bioassays were performed using 20 snails per site. A control group with tap water was added. Egg masses were separated, exposed individually and observed daily using a stereoscopic microscope. After hatching, juveniles were placed in tap water and offspring survival was registered at the first, second, third and fourth months after the beginning of the assay. High levels of conductivity, turbidity and nutrients were obtained. Ammonium and nitrite were higher than the guideline level for the protection of aquatic life. During the bioassay 20% of the snails (L2 and L3) showed abnormally protruding of the head-food region. The number of eggs and embryonated eggs per mass did not differ between treatments. Egg masses exposed to water samples from the lake presented overlapping and abnormal eggs and arrested embryos. Besides, low % of hatching (L1: 33%, L2: 42%, and L3: 16%) and juvenile survival after the first (L1:14%; L2:78%) and second month (L1: 60%) were noted. In the control group, 85% of hatching and 100%–90% of survival were observed. Our results suggests the presence of pollutant in the lake. B. straminea seems to be a sensitive local species. Biomphalaria spp. reproduction assays can provide a valuable endpoint for toxicity and risk assessments and a usefulness tool for biomonitoring water quality.

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Section: Discussionmentioning

confidence: 94%

“…Biomphalaria spp. have been indicated as useful models for assessing chemical toxicity using early development stages mainly during the embryogenesis process ( Caixeta et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aquatic Contamination in Lugano Lake (Lugano Lake Ecological Reserve, Buenos Aires, Argentina) Cause Negative Effects on the Reproduction and Juvenile Survival of the Native Gastropod Biomphalaria straminea

et al. 2022

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Using freshwater snail Biomphalaria glabrata (Say, 1818) as a biological model for ecotoxicology studies: a systematic review

Souza-Silva

Souza

Pereira

et al. 2023

Environ Sci Pollut Res

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Over time, a growing increase in human pollutants in the aquatic environment has been observed. The global presence of residues in water bodies reinforces the need to develop improved methods to detect them and evaluate their ecotoxicological effects in the aquatic environment. Thus, this study aimed to present the main assays using B. glabrata as a biological model for ecotoxicological studies. We performed a systematic literature review with data published up to June 2022 in the Science Direct, PubMed, and SciELO databases. Twenty-six studies were selected for this review after screening. B. glabrata has been studied as an ecotoxicological model for different substances through toxicity, embryotoxicity, cytotoxicity, genotoxicity and bioaccumulation assays. Studies evaluating the impact of Biomphalaria glabrata exposure to several substances have reported toxic effects, behavioral and reproductive effects, and effects on their offspring. This review presents various assays using Biomphalaria glabrata as a biological model for ecotoxicological studies. The increasing number of studies conducted with the snail provides a preview of the snail's potential as a biological model for ecotoxicological studies.

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Hazardous effects of nickel ferrite nanoparticles and nickel chloride in early life stages of the freshwater snail Biomphalaria glabrata (Say, 1818)

Pena,

Silva Brito,

Araújo

et al. 2024

Environ Sci Pollut Res

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Biomphalaria embryotoxicity test (BET): 60 years of research crossing boundaries for developing standard protocols

Cited by 11 publications

References 81 publications

Aquatic Contamination in Lugano Lake (Lugano Lake Ecological Reserve, Buenos Aires, Argentina) Cause Negative Effects on the Reproduction and Juvenile Survival of the Native Gastropod Biomphalaria straminea

Aquatic Contamination in Lugano Lake (Lugano Lake Ecological Reserve, Buenos Aires, Argentina) Cause Negative Effects on the Reproduction and Juvenile Survival of the Native Gastropod Biomphalaria straminea

Using freshwater snail Biomphalaria glabrata (Say, 1818) as a biological model for ecotoxicology studies: a systematic review

Hazardous effects of nickel ferrite nanoparticles and nickel chloride in early life stages of the freshwater snail Biomphalaria glabrata (Say, 1818)

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