Biomonitoring of the genotoxic potential (micronucleus and erythrocyte nuclear abnormalities assay) of the Admiralty Bay water surrounding the Brazilian Antarctic Research Station “Comandante Ferraz,” King George Island

Ngan, Phan Van; Gomes, Vicente; Passos, M J A C R; Ussami, Keyi Ando; Campos, Denise; Rocha, Arthur José da Silva; Pereira, Bruna A.

doi:10.1007/s00300-006-0174-x

Cited by 35 publications

(24 citation statements)

References 44 publications

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“…The important here is that this data on the total ENMAs suggested a scenario of greater exposure to genotoxic contamination in the Douro and Ave estuaries. Other field surveys that used total ENMAs to access such type of contamination support our view (no matter what combination of nuclear abnormalities was ultimately included in the total counts) (Cavalcante et al 2008;Van Ngan et al 2007). …”

Section: Discussionsupporting

confidence: 71%

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Carrola

Santos

Rocha

et al. 2014

Environ Sci Pollut Res

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Fish are bioindicators of water pollution, and an increased rate of their erythrocyte nuclear morphological abnormalities (ENMAs)-and particularly of erythrocyte micronuclei (EMN)-is used as a genotoxicity biomarker. Despite the potential value of ENMAs and MN, there is scarce information about fish captured in Iberian estuaries. This is the case of the Portuguese estuaries of the Mondego, Douro and Ave, suffering from different levels of environmental stress and where chemical surveys have been disclosing significant amounts of certain pollutants. So, the aim of this study was to evaluate genotoxicants impacts and infer about the exposure at those ecosystems, using the grey mullet (Mugil cephalus) as bioindicator and considering the type and frequency of nuclear abnormalities of erythrocytes as proxies of genotoxicity. Sampling of mullets was done throughout the year in the important Mondego, Douro and Ave River estuaries (centre and north-western Portugal). The fish (total n=242) were caught in campaigns made in spring-summer and autumnwinter, using nets or fishing rods. The sampled mullets were comparable between locations in terms of the basic biometric parameters. Blood smears were stained with Diff-Quik to assess the frequencies of six types of ENMAs and MN (given per 1,000 erythrocytes). Some basic water physicochemical parameters were recorded to search for fluctuations matching the ENMAs. Overall, the most frequent nucleus abnormality was the polymorphic type, sequentially followed by the blebbed/ lobed/notched, segmented, kidney shaped, vacuolated, MN and binucleated. The total average frequency of the ENMAs ranged from 73‰ in the Mondego to 108‰ in the Ave. The polymorphic type was typically ≥50 % of the total ENMAs, averaging about 51‰, when considering all three estuaries. The most serious lesion-the MN-in fish from Mondego and Douro had a similar frequency (≈0.38‰), which was significantly lower than that in the Ave (0.75‰). No significant seasonal differences existed as to the MN rates and seasonal differences existed almost only in the Douro, with the higher values in AW. In general, the pattern of ENMAs frequencies was unrelated with the water physicochemical parameters. Considering the data for both the total ENMAs and for each specific abnormality, and bearing in mind that values of MN in fish erythrocytes >0.3‰ usually reflect pollution by genotoxicants, it is suggested that mullets were likely being chronically exposed to such compounds, even in the allegedly less polluted ecosystem (Mondego). Moreover, data supported the following pollution

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Section: Discussionsupporting

confidence: 71%

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Carrola

Santos

Rocha

et al. 2014

Environ Sci Pollut Res

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“…ENA frequency in each blood sample was scored by scanning the smears under microscope (1009 objective) per 10,000 mature erythrocytes (Schmid 1975). Nuclear abnormalities observed were micronucleated erythrocytes (MN) ( (Kursa and Bezrukov 2007;Van Ngan et al 2007). Erythrocytes with other nuclear malformations were classified as unknown.…”

Section: Methodsmentioning

confidence: 99%

“…In recent decades, many studies on toxic substances have shown significant presence of noxious products in Antarctic wildlife, specifically in south polar skuas (Tao et al 2006;Kursa and Bezrukov 2007), penguins (Van den Brink 1997;Corsolini et al 2007;Geisz et al 2008;Schiavone et al 2009;Jerez et al 2011;Barbosa et al 2013;Jerez et al 2013a, b), albatrosses (Tao et al 2006), petrels (Van den Brink 1997), seals (Tao et al 2006;Schiavone et al 2009), whales (Krahn et al 2008), fishes (Van Ngan et al 2007), krill (Corsolini et al 2006;Jerez et al 2013a, b), and lichens and mosses (Yogui and Sericano 2008).…”

Section: Introductionmentioning

confidence: 97%

“…The other nuclear abnormalities result from analogous damage (Schmid 1975;Fenech 2000). ENA detection has been used successfully to test for and report exposure to radiation (Muller et al 1996) and genotoxic substances (i.e., PAHs, heavy metals and POPs) and evaluate their effects on organisms such as fish (Cavas and Ergene-Gozukara 2005;Matsumoto et al 2006;Ergene et al 2007;Van Ngan et al 2007; Guilherme et al 2008), birds (Quirós et al 2008,) and amphibians (Marques et al 2009). Moreover, some of these studies have used ENAs in birds as baseline data in order to do long-term comparisons of environmental deterioration and pollution in specific areas (Kursa and Bezrukov 2007).…”

Section: Introductionmentioning

confidence: 99%

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Erythrocytic abnormalities in three Antarctic penguin species along the Antarctic Peninsula: biomonitoring of genomic damage

et al. 2015

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Pollutants and toxic contaminants produced in all parts of the world are transported to remote regions including Antarctica. Tourism, research, and fishing activities on this continent are another source of contamination. Toxic substances affect Antarctic species, and some produced genomic damage to the fauna. The genetic damage can be detected by microscopic observation of erythrocytic nuclear abnormalities (ENAs). We counted the number of ENAs in seven populations of three Pygoscelid penguin species, Adélie (Pygoscelis adeliae), Chinstrap (Pygoscelis antarctica), and Gentoo (Pygoscelis papua), and found important differences among species exposed to the same conditions. ENAs were more frequent in Adélie penguins than in the other two species.

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“…Environmental monitoring of Antarctic regions occupied by signatory countries is an objective of the Antarctic Treatise (Santos et al, 2006;Phan et al, 2007;Gomes et al, 2009Gomes et al, , 2012. The Brazilian Antarctic Program has studied methods for environmental monitoring that assesses and mitigates impacts caused by the human presence to the environment and to the organisms that inhabit it (Martins et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Biomonitoring of Genotoxicity of Shallow Waters Around the Brazilian Antarctic Station Comandante Ferraz (Eacf), Admiralty Bay, King George Island, Antarctica, Using Amphipod Crustaceans

Gomes¹,

Rocha²,

Passos³

et al. 2015

INCT-APA Annual Activity Report

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The comet assay was applied to the biomonitoring of genotoxicity in shallow waters around the Brazilian Antarctic Station "Comandante Ferraz" (EACF). Mean values of DNA damage to animals captured from shallow waters nearby the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) were significantly higher than the values of the controls captured from shallow waters of Punta Plaza (PPL) and Yellow Point (YP), naturally undisturbed places far from the EACF.

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Biomonitoring of the genotoxic potential (micronucleus and erythrocyte nuclear abnormalities assay) of the Admiralty Bay water surrounding the Brazilian Antarctic Research Station “Comandante Ferraz,” King George Island

Cited by 35 publications

References 44 publications

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Erythrocytic abnormalities in three Antarctic penguin species along the Antarctic Peninsula: biomonitoring of genomic damage

Biomonitoring of Genotoxicity of Shallow Waters Around the Brazilian Antarctic Station Comandante Ferraz (Eacf), Admiralty Bay, King George Island, Antarctica, Using Amphipod Crustaceans

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