Biomonitoring of the genotoxic potential (micronucleus assay) and detoxifying activity (EROD induction) in the River Dadou (France), using the amphibian Xenopus laevis

Gauthier, Laury; Tardy, Emmanuelle; Mouchet, Florence; Marty, J.

doi:10.1016/j.scitotenv.2003.10.014

Cited by 56 publications

(37 citation statements)

References 40 publications

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“…The use of biomarkers has become attractive and useful for monitoring environmental quality and the health of fish inhabiting polluted ecosystems (Gauthier et al 2004;Ozmen et al 2006;Köhler et al 2007). Within this context, fish have been widely used in toxicologic pathology as models to evaluate the health of aquatic ecosystems (Law 2003).…”

Section: Introductionmentioning

confidence: 99%

Monitoring pollution in Esmoriz–Paramos lagoon, Portugal: Liver histological and biochemical effects in Liza saliens

Fernandes

Fontaínhas‐Fernandes

Rocha

et al. 2007

Environ Monit Assess

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The Esmoriz-Paramos lagoon is one important ecosystem located in the Northwest coast of Portugal. This study is concerned with the assessment of pollution of the lagoon contributed by urban, industrial and agricultural activities. The parameters selected for this aim were the occurrence of hepatic histological alterations and plasma blood biochemistry in Liza saliens, the dominant fish in this lagoon. Blood parameters were compared between mullets collected from the lagoon and from the sea and included plasma enzyme activities (aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP)), glucose (GLU) and total protein (TP). A positive relationship between hepatocyte vacuolization index and hepatosomatic index was found, suggesting that lipid accumulation could have contributed to the increase of liver weight. The hepatic alterations recorded included general diagnostic categories and additionally large areas of heterogeneous parenchyma, composed of hepatocytes with different spectrum of vacuolization. Liver histopathological evaluation revealed a sample prevalence of 34% for heterogeneous parenchyma, 31% for foci of necrosis and 9% for non-neoplastic lesions. No neoplastic lesions were found. Livers with heterogeneous parenchyma showed higher Cu and Zn content than livers without this category of lesion, suggesting that metals accumulated could trigger this hepatic alteration. AST was higher in mullets from the lagoon than in mullets from the sea, but no differences in ALP and ALT were found, suggesting a change in protein metabolism as an adaptive response to metals exposure. The higher glucose and protein contents observed in fish caught in the lagoon are consistent with a stress response and the measurement of plasma AST activity could be a sensitive indicator of lagoon fish stress. In conclusion, Esmoriz-Paramos lagoon biomonitorization, using indicator species will proceed to assess the impact of the restoration program under development by the Portuguese government.

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Section: Introductionmentioning

confidence: 99%

Monitoring pollution in Esmoriz–Paramos lagoon, Portugal: Liver histological and biochemical effects in Liza saliens

Fernandes

Fontaínhas‐Fernandes

Rocha

et al. 2007

Environ Monit Assess

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“…Increasing awareness of the adverse effects of anthropogenic activities and pollution on aquatic environment has focused interest on health of fish populations and possibilities to utilize these health parameters for assessment of the quality of aquatic environment (Henry et al, 2004). The potential utility of biomarkers for monitoring both environmental quality and the health of organisms inhabiting polluted ecosystems has received increasing attention during recent years (Lopes et al, 2001;Samecka-Cymerman and Kempers, 2003;Gauthier et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Monitoring the Effects of Water Pollution on Cyprinus carpio in Karakaya Dam Lake, Turkey

et al. 2005

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Karakaya Dam Lake (KDL) is one of the most important water sources, both for irrigation and fishery, located in eastern part of Turkey. This study is concerned with the pollution of the lake contributed by urban, industrial and agricultural activities. The parameters selected for this aim were the enzymes commonly used as biomarkers of environmental pollution. The activity of glutathione S-transferase (GST), carboxylesterase (CE), lactate dehydrogenase (LDH), acid phosphatase (ACP) and aspartate amino transferase (AST) has been determined in liver tissue samples of Cyprinus carpio, a representative species of KDL. Furthermore, brain acetylcholinesterase (AChE) activity which is mainly affected by pesticides such as organophosphates, has been assayed. Chemical analysis results showed that KDL was polluted by various heavy metals as it was apparent from water, sediment and gill tissue. The activity of brain AChE was significantly lower in all localities than Tecimli area (St-5) where there is no agricultural and industrial activities in the immediate periphery. Thus, this change of AChE activity may relate to agricultural pollution in KDL. On the other hand, no significant differences were found for selected enzyme biomarkers, but condition factor (CF) or hepatosomatic index were significantly different from the St-5 samples, a result that may be attributed to water pollution in KDL by various contaminants.

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“…In particular, the ecotoxicological relevance of the larvae of Xenopus laevis has been well established in many studies on biomarkers such as the induction of biotransformation enzyme activities (Gauthier et al, 2004), micronucleus formation (Ferrier et al, 1998), primary DNA damage (Mouchet et al, 2005(Mouchet et al, , 2006(Mouchet et al, , 2007, adduct formation (Békaer et al, 2002), and teratogenic malformations with the FETAX assay (Chenon et al, 2002). Finally, X. laevis larvae have been used to evaluate stress inductive potential in response to Cadmium using relevant combined endpoints such as micronucleus induction, MT levels, bioaccumulation and gene expression (Mouchet et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Recovery capabilities of Xenopus laevis after exposure to Cadmium and Zinc

et al. 2015

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Biomonitoring of the genotoxic potential (micronucleus assay) and detoxifying activity (EROD induction) in the River Dadou (France), using the amphibian Xenopus laevis

Cited by 56 publications

References 40 publications

Monitoring pollution in Esmoriz–Paramos lagoon, Portugal: Liver histological and biochemical effects in Liza saliens

Monitoring pollution in Esmoriz–Paramos lagoon, Portugal: Liver histological and biochemical effects in Liza saliens

Monitoring the Effects of Water Pollution on Cyprinus carpio in Karakaya Dam Lake, Turkey

Recovery capabilities of Xenopus laevis after exposure to Cadmium and Zinc

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