Biomonitoring for assessment of organic dust-induced lung inflammation

Mueller-Anneling, Linda; O'Neill, Marsha E.; Thorne, Peter S.

doi:10.1183/09031936.06.00092204

Cited by 23 publications

(16 citation statements)

References 42 publications

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“…In our study, blood was collected from Monday to Wednesday at the start of the workday, and there was no association between day of the week and cytokine production (p.0.3). A lower response in the WBA has been described in mice that had developed tolerance to LPS [28], but, in human studies including the present one, there is no evidence of development of tolerance at adult age to occupational endotoxin exposure, at least not at the level of cytokine responses [27].…”

Section: Discussioncontrasting

confidence: 61%

Ex vivo cytokine release reflects sensitivity to occupational endotoxin exposure

Smit¹,

Heederik²,

Doekes³

et al. 2009

European Respiratory Journal

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Not everyone exposed to endotoxin develops respiratory symptoms, even at very high exposure levels. The aim was to investigate whether ex vivo lipopolysaccharide (LPS)-induced cytokine release may be predictive of individual sensitivity to occupational endotoxin exposure.In 412 agricultural workers, tumour necrosis factor (TNF)-a, interleukin (IL)-1b and IL-10 release was measured in supernatants from LPS-stimulated whole blood, lung function was measured, and respiratory symptoms were assessed by questionnaire. For each cytokine, the population was dichotomised into low and high responders according to median cytokine concentrations. Endotoxin exposure levels were determined based on 249 personal exposure measurements.High IL-10 responders had a higher prevalence of airway symptoms than low IL-10 responders (odds ratios between 2.03 and 5.10; p,0.05). TNF-a response was positively, but not significantly, associated with symptoms, whereas no relationship was found between IL-1b response and symptoms. For all three cytokines, subjects with above-median responses showed significant positive dose-response relationships between endotoxin exposure and asthma symptoms, and significant associations between endotoxin exposure and a lower forced expiratory volume in 1 s (p,0.05). In contrast, exposure-response relationships were weak and statistically nonsignificant for low responders.The ex vivo inflammatory response to LPS reflects, to a large extent, whether individuals are susceptible to adverse respiratory effects induced by high occupational endotoxin exposure.

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Section: Discussioncontrasting

confidence: 61%

Ex vivo cytokine release reflects sensitivity to occupational endotoxin exposure

Smit¹,

Heederik²,

Doekes³

et al. 2009

European Respiratory Journal

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“…These manufactured nanoparticles, with the highest commercially available surface area and smallest particle size for TiO 2, did not show particularly toxic effects in this subacute inhalation study. In contrast, this murine model has demonstrated robust inflammatory responses upon inhalation of grain dust (Jagielo et al 1996; Mueller-Anneling et al 2006) or endotoxin (Thorne et al 1999) with a high number of total cells, neutrophils, or IL-6 or TNF-α levels in BAL fluid. It should be noted, with short-term inhalation exposures in rats to iron nanomaterials, inflammatory responses were also minimal (Zhou et al 2003).…”

Section: Discussionmentioning

confidence: 95%

Inhalation Exposure Study of Titanium Dioxide Nanoparticles with a Primary Particle Size of 2 to 5 nm

Grassian

O’Shaughnessy

Adamcakova‐Dodd

et al. 2007

Environ Health Perspect

387

234

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BackgroundNanotechnology offers great promise in many industrial applications. However, little is known about the health effects of manufactured nanoparticles, the building blocks of nanomaterials.ObjectivesTitanium dioxide (TiO2) nanoparticles with a primary size of 2–5 nm have not been studied previously in inhalation exposure models and represent some of the smallest manufactured nanoparticles. The purpose of this study was to assess the toxicity of these nanoparticles using a murine model of lung inflammation and injury.Materials and MethodsThe properties of TiO2 nanoparticles as well as the characteristics of aerosols of these particles were evaluated. Mice were exposed to TiO2 nanoparticles in a whole-body exposure chamber acutely (4 hr) or subacutely (4 hr/day for 10 days). Toxicity in exposed mice was assessed by enumeration of total and differential cells, determination of total protein, lactate dehydrogenase (LDH) activity and inflammatory cytokines in bronchoalveolar lavage (BAL) fluid. Lungs were also evaluated for histopathologic changesResultsMice exposed acutely to 0.77 or 7.22 mg/m3 nanoparticles demonstrated minimal lung toxicity or inflammation. Mice exposed subacutely (8.88 mg/m3) and necropsied immediately and at week 1 or 2 postexposure had higher counts of total cells and alveolar macrophages in the BAL fluid compared with sentinels. However, mice recovered by week 3 postexposure. Other indicators were negative.ConclusionsMice subacutely exposed to 2–5 nm TiO2 nanoparticles showed a significant but moderate inflammatory response among animals at week 0, 1, or 2 after exposure that resolved by week 3 postexposure.

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“…Poole et al (2009b) reported increased cellular inflammation, predominated by neutrophils and neutrophils and macrophages, in mouse BALF following a single and 1/2 week intranasal instillation of DE, respectively. Mice continuously exposed to swine CAFO dust via nebulizer for 4 h developed BALFneutrophilia (Mueller-Anneling et al, 2006). The role of macrophages present in the lungs of mice chronically treated with DE is not known.…”

Section: Discussionmentioning

confidence: 99%

Exposure to Swine Housing Dust Modulates Macrophage Morphology and Function

Pender

Minor

Hurley

et al. 2014

American Journal of Immunology

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Swine Confinement Facility (SCF) dust consists of a complex mixture of feed grain particles, bacterial components, organic particulates and gases. When these particles are inhaled they deposit along the respiratory tract and mediate respiratory symptoms and disease in swine farmers and facility workers. Macrophages ingest and eliminate microbes and debris under chronic conditions; however, the role of macrophages in agricultural-related respiratory disease has not been fully elucidated. The goal was to evaluate the hypothesis that chronic exposure to SCF dust causes inflammation by modulating pulmonary protein levels and macrophage function. Balb/c mice were exposed to 5, 12.5 and 25% SCF Dust Extract (DE) via nebulization 30 min/day five days a week, for eight weeks with weekends excluded. Bronchoalveolar Lavage Fluid (BALF) was collected and analyzed for protein concentration, leukocyte distribution and macrophage morphology. For comparison, THP-1 monocytic cells were exposed to 0.1-10% DE overnight and evaluated for phagocytosis and reactive oxygen species production. Repeated exposure to DE via nebulizer caused a significant increase in protein concentration and inflammatory cell number, namely macrophages, in a dose-dependent manner within the lung as compared to controls. Macrophages with pseudopods and vacuoles were the most abundant leukocytes within BALF of mice exposed to DE. Similarly, in vitro studies with 10% DE treated THP-1 cells revealed enhanced phagocytosis (p<0.05), pseudopodia and vacuolization following exposure to compared to control cells. In addition, there were time-and dose-dependent increases of intracellular ROS production by THP-1 cells exposed to 5 and 10% DE compared to control (p<0.01). These findings indicate repeated, long-term inhalation of swine confinement facility dust may mediate chronic airway and lung inflammation through modulation of protein concentration and macrophage function. The aerosolized dust-mouse inhalation model presented here may offer a good tool for studying particle mediated chronic inflammation of the tracheobronchial tree and lungs.

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Biomonitoring for assessment of organic dust-induced lung inflammation

Cited by 23 publications

References 42 publications

Ex vivo cytokine release reflects sensitivity to occupational endotoxin exposure

Ex vivo cytokine release reflects sensitivity to occupational endotoxin exposure

Inhalation Exposure Study of Titanium Dioxide Nanoparticles with a Primary Particle Size of 2 to 5 nm

Exposure to Swine Housing Dust Modulates Macrophage Morphology and Function

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