Biomolecular NMR Spectroscopy of Ribonucleic Acids

Abstract

Biomolecular nuclear magnetic resonance (NMR) spectroscopy allows the characterization of structural and dynamic properties of ribonucleic acids (RNAs) in solution. The NMR‐based determination of high‐resolution three‐dimensional (3D) structures has been achieved for RNAs up to ca . 100 nucleotides. Biomolecular NMR also provides valuable information about RNA–ligand interactions and the role of metal ions and shifted p K a in RNA structure and catalysis.