Biomolecular release triggered by glucose input – bioelectronic coupling of sensing and actuating systems

Mailloux, Shay; Halámek, Jan; Halámková, Lenka; Tokarev, Alexander; Minko, Sergiy; Katz, Evgeny

doi:10.1039/c3cc42027b

Cited by 33 publications

(45 citation statements)

References 32 publications

(44 reference statements)

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“…In addtion, it is possible to replace NADH with other reducing molecules (e.g., glucose). [25] The deoxyribozyme gate-based computational systems are also known to show great versatility and complexity. [20-22] The limitations of the interface are the following.…”

mentioning

confidence: 99%

Bridging the Two Worlds: A Universal Interface between Enzymatic and DNA Computing Systems

et al. 2015

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Molecular computing based on enzymes or nucleic acids has attracted a great deal of attention due to the perspectives of controlling living systems in a way we control electronic computers. Enzyme-based computational systems can respond to a great variety of small molecule inputs. They have an advantage of signal amplification and highly specific recognition. DNA computing systems are most often controlled by oligonucleotide inputs/outputs and are capable of sophisticated computing, as well as controlling gene expressions. Here, we developed an interface that enables communication of otherwise incompatible nucleic acid and enzyme computational systems. The enzymatic system processes small molecules as inputs and produces NADH as an output. The NADH output triggers electrochemical release of an oligonucleotide, which is accepted by a DNA computational system as an input. This interface is universal since the enzymatic and DNA computing systems are independent of each other in composition and complexity.

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confidence: 99%

Bridging the Two Worlds: A Universal Interface between Enzymatic and DNA Computing Systems

et al. 2015

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“…potentiostat) 87 or by generating negative potential in situ by biocatalytic reactions. 20 In the present work 86 the negative potential and reductive current for the Fe +3 -alginate film was generated by a pyrroloquinoline quinone (PQQ)-modified electrode biocatalytically oxidizing NADH. 88 Using NADH as the electron donating species for the biocatalytic electrode allows large versatility for the signal-processing system because NADH can be generated in situ by numerous biocatalytic systems logically processing various combinations of biomolecular signals.…”

Section: Integration Of a Bioelectrochemical Actuator With A Biomolecmentioning

confidence: 99%

“…While computational applications of biomolecular systems [14][15][16] competing with modern electronics are rather futuristic, their use in low scale information processing for biosensing [17][18][19] and bioactuating, [20][21][22] particularly aiming at medical use [23][24][25][26] and benefiting from operation in a biological environment, 27,28 are feasible already at the present level of technology. Rapid progress in the enzyme-based information processing systems resulted in the design of biocatalytic cascades mimicking various Boolean logic gates, 1 including AND, [29][30][31][32][33][34][35][36] OR, 34,36,37 NAND, 38,39 NOR, 36,39 CNOT, 40 XOR, 34,36,41,42 INHIBIT, 34,36 Identity 36 and Inverter 36 gates.…”

Section: Introductionmentioning

confidence: 99%

Biomolecular logic systems: applications to biosensors and bioactuators

Katz

2014

SPIE Proceedings

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The paper presents an overview of recent advances in biosensors and bioactuators based on the biocomputing concept. Novel biosensors digitally process multiple biochemical signals through Boolean logic networks of coupled biomolecular reactions and produce output in the form of YES/NO response. Compared to traditional single-analyte sensing devices, biocomputing approach enables a high-fidelity multi-analyte biosensing, particularly beneficial for biomedical applications. Multi-signal digital biosensors thus promise advances in rapid diagnosis and treatment of diseases by processing complex patterns of physiological biomarkers. Specifically, they can provide timely detection and alert to medical emergencies, along with an immediate therapeutic intervention. Application of the biocomputing concept has been successfully demonstrated for systems performing logic analysis of biomarkers corresponding to different injuries, particularly exemplified for liver injury. Wide-ranging applications of multi-analyte digital biosensors in medicine, environmental monitoring and homeland security are anticipated. "Smart" bioactuators, for example for signal-triggered drug release, were designed by interfacing switchable electrodes and biocomputing systems. Integration of novel biosensing and bioactuating systems with the biomolecular information processing systems keeps promise for further scientific advances and numerous practical applications.

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“…The Fe 3 + -reducing potentials could be applied by a potentiostat [29] or produced in situ by using biocatalytic reactions. [30] In the present system, the alginate-modified electrode was electrically connected with the PQQ-modified electrode, which produced the reducing potential in the presence of NADH, dissolving the alginate film and releasing the entrapped enzymes. cross-linked alginate polymer film when it was electrochemically formed.…”

Section: Application Of Systems Controlled By Logical Biomolecular Simentioning

confidence: 99%

Starch‐Powered Biofuel Cell Activated by Logically Processed Biomolecular Signals

Mailloux¹,

MacVittie²,

Privman³

et al. 2014

ChemElectroChem

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An overview of the application of signal‐switchable electrodes in biofuel cells is provided, concentrating on sophisticated systems where logically processed biomolecular signals can activate power production by switchable biofuel cells. The discussion is directed to the formulation of a new concept of physiologically controlled biofuel cells with the power production on demand, particularly in the case of implantable biofuel cells operating in vivo under physiological conditions. The practical realization of the novel concept may be in separating the signal‐processing and power‐producing sub‐systems which communicate electronically. The general conceptual discussion is exemplified with a system where a biomolecular logic system represented by a single Boolean OR logic gate is used to activate enzyme‐release to produce glucose‐biofuel from starch to activate power generation by a biofuel cell. The presented example is aiming at the concept demonstration rather than immediate practical application. Further sophistication of the given example is possible based on the rapidly developing biocomputing approach and integration of signal‐processing systems with switchable materials, electrodes and biofuel cells.

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Biomolecular release triggered by glucose input – bioelectronic coupling of sensing and actuating systems

Cited by 33 publications

References 32 publications

Bridging the Two Worlds: A Universal Interface between Enzymatic and DNA Computing Systems

Bridging the Two Worlds: A Universal Interface between Enzymatic and DNA Computing Systems

Biomolecular logic systems: applications to biosensors and bioactuators

Starch‐Powered Biofuel Cell Activated by Logically Processed Biomolecular Signals

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