Biomolecular motors at the intersection of nanotechnology and polymer science

Agarwal, Ashutosh; Hess, Henry

doi:10.1016/j.progpolymsci.2009.10.007

Cited by 144 publications

(131 citation statements)

References 272 publications

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“…the actomyosin system which also has been extensively investigated for molecular motorbased devices. Characteristics, such as a range of motion speeds and flexural rigidity, of each of the systems are complementary to each other (Agarwal and Hess, 2010;Månsson et al, 2012). Thus the glass wire templates, with its inherent simplicity of production and handling, may be beneficial not only for interfacing macroscale fluidic delivery with molecular-level transportation, but also for potential hybridization of different motility systems.…”

Section: Discussionmentioning

confidence: 99%

“…Collective operation of the motor domains holding one microtubule results in its translocation, allowing its use as a potential transporter (Brunner et al, 2007). This cooperative mode has a great advantage in that the distance of travel of microtubules is several orders of magnitude longer compared to the run lengths of an individual kinesin (Agarwal and Hess, 2010;Muthukrishnan et al, 2009). However, for a practical application of the biomotile system randomness in the initial gliding direction of microtubules still needs to be overcome.…”

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confidence: 99%

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Microtubule shuttles on kinesin-coated glass micro-wire tracks

Kim

Liao

Sikora

et al. 2014

Biomed Microdevices

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Gliding of microtubule filaments on surfaces coated with the motor protein kinesin has potential applications for nano-scale devices. The ability to guide the gliding direction in three dimensions allows the fabrication of tracks of arbitrary geometry in space. Here, we achieve this by using kinesin-coated glass wires of micrometer diameter range. Unlike previous methods in which the guiding tracks are fixed on flat two-dimensional surfaces, the flexibility of glass wires in shape and size facilitates building in-vitro devices that have deformable tracks.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Microtubule shuttles on kinesin-coated glass micro-wire tracks

Kim

Liao

Sikora

et al. 2014

Biomed Microdevices

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“…The resulting mixture was submitted for silica column chromatography using a mixed solvent (DCM / EtOAc, 9:1) and 13 was obtained in 91% yield. 1 was verified by the measurements of contact angle before and after deprotection.…”

Section: Synthesismentioning

confidence: 80%

“…Yield: 79%. 1 and potassium carbonate (2.8 g, 38 mmol) was dissolved in acetone (60 mL) and the mixture was refluxed for 12 hours under argon atmosphere. The mixture was then diluted with brine and was extracted with ethyl acetate (EtOAc); the combined extracts were dried (MgSO4) and the solvent was removed under reduced 75 pressure.…”

Section: Synthesismentioning

confidence: 99%

Dynamic photo-control of kinesin on a photoisomerizable monolayer – hydrolysis rate of ATP and motility of microtubules depending on the terminal group

2012

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The reversibly and repeatedly altered gliding motility of microtubules driven by kinesin on the photoresponsive monolayer surface is studied. It was confirmed that an azobenzene monolayer surface needs to have free amino terminal groups for the successful dynamic control of the motility of microtubule. The surface of azobenzene monolayer with terminal amino groups can dynamically control 10 the ATP hydrolysis activity of kinesin which resulted in the change in motility of microtubule.

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“…Currently, the in vitro motility assay is also used to determine the feasibility of constructing motor protein-based artificial biomachines, for which actin-myosin or MT-kinesin systems are considered promising building blocks. [8][9][10][11][12][13][14][15][16] New classes of devices including nanoscale molecular shuttles, 17,18 surface-imaging processes, 19 force measurements 20 and lab-on-a-chip devices 21 have been developed using knowledge obtained through the in vitro motility assay. To improve the organizational hierarchy of motor protein-based systems with emergent functions similar to those observed in natural systems, several active self-organization (AcSO) techniques have been developed.…”

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confidence: 99%

Active self-organization of microtubules in an inert chamber system

Kabir

Inoue

Kakugo

et al. 2012

Polym J

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Microtubule-kinesin system is considered as a building block for the construction of artificial biomachines, and active selforganization of microtubules has been used to integrate their structural organization and achieve amplified functions similar to those observed in natural systems. However, the short lifetime of assembled structures has limited their use in organized systems. In the present study, we demonstrated that the use of an inert atmosphere in the self-organization of microtubules allows the assembled structures to remain active for a prolonged period of time (10 times longer). The longer lifetime achieved in the present study will facilitate the development of assembled microtubules for designing biomolecular motor-based efficient artificial biomachines with prolonged lifetimes. Polymer Journal ( Keywords: active self-organization; biomachine; biomolecular motor; kinesin; lifetime; microtubule INTRODUCTION Actin-myosin and microtubule (MT)-kinesin systems are known for their fascinating in vivo activities with respect to cell motility, 1 cytokinesis 2 and cellular transport. To unveil the functions of bimolecular motor systems 3-5 and to elucidate the in vivo mechanism of actin-myosin or MT-kinesin interactions, in vitro motility assay 6,7 has been used over the last few decades. Currently, the in vitro motility assay is also used to determine the feasibility of constructing motor protein-based artificial biomachines, for which actin-myosin or MT-kinesin systems are considered promising building blocks. [8][9][10][11][12][13][14][15][16] New classes of devices including nanoscale molecular shuttles, 17,18 surface-imaging processes, 19 force measurements 20 and lab-on-a-chip devices 21 have been developed using knowledge obtained through the in vitro motility assay. To improve the organizational hierarchy of motor protein-based systems with emergent functions similar to those observed in natural systems, several active self-organization (AcSO) techniques have been developed. [22][23][24][25] For instance, a specific streptavidin (St)-biotin (Bt) interaction has been used in the in vitro motility assay of MTs on a kinesin-fixed surface, and a variety of well-organized MTs assemblies have been produced including linear bundles, rings and network structures, all of which showed motility comparable to that of single MT filaments. However, the lifetime of biomolecular motors is short owing to the attack of reactive oxygen species (ROS), which terminates their activity and limits the long-term utilization of assembled structures. Thus, assembled structures formed under ambient aerobic conditions remain active for only B90 min.

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Biomolecular motors at the intersection of nanotechnology and polymer science

Cited by 144 publications

References 272 publications

Microtubule shuttles on kinesin-coated glass micro-wire tracks

Microtubule shuttles on kinesin-coated glass micro-wire tracks

Dynamic photo-control of kinesin on a photoisomerizable monolayer – hydrolysis rate of ATP and motility of microtubules depending on the terminal group

Active self-organization of microtubules in an inert chamber system

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