Biomolecular Modulation of Neurodegenerative Events during Ageing

Abstract

The objective is to assess the modulation of retinal and optic nerve degenerative events induced by the combination of α-lipoic acid (ALA) and superoxide dismutase (SOD) in an animal model of ageing. For this study, 24 male Wistar-Harlan strain rats were left to age for up to 24 months. One group of rats was subjected to a diet supplemented with ALA and SOD for 8 weeks, while another group was used as a positive control and not subjected to any dietary treatment. To assess the cytoprotective effects of the antioxidants, a morphological analysis was carried out on sections of retina and optic nerve head, stained with haematoxylin-eosin, followed by an analysis of the modifications to nuclear DNA detected by the TUNEL technique. The lipid peroxidation assay was used to assess the damage induced by oxidative stress at cell membrane level. The molecules involved in apoptosis mediated by oxidative stress, such as caspase-3 and inducible nitric oxide synthase, were also assayed by immunolocalization and western blot. ALA and SOD are able to counteract senile neurodegenerative deterioration to the retina and optic nerve. Indeed, the combination of these antioxidant molecules can reduce oxidative stress levels and thus prevent both nuclear degradation and subsequent cell death.

Die alternde Retina im Kontext zerebraler neurodegenerativer Erkrankungen

“…Ein Großteil der publizierten Arbeiten beschäftigt sich mit der postnatalen Entwicklung und dem Altern des visuellen Kortexes [5,6]. Die dort ablaufenden zeitlichen Verläufe des Wachstums und der Formierung von basalen Dendriten der pyramidalen Zellen unterscheiden sich deutlich von sensorischen, assoziativen und exekutiven kortikalen Arealen [6,7]. Die hier vorhandenen zeitlichen Zusammenhänge können klinisch mit der postnatalen Entwicklung der Sehfähigkeit und mit einer möglichen Amblyopieentwicklung korreliert werden [7].…”

Retracted: Biomolecular Modulation of Neurodegenerative Events during Ageing

“…Oxidative Medicine and Cellular Longevity has retracted the article titled “Biomolecular Modulation of Neurodegenerative Events during Ageing” [1]. As raised on PubPeer, the article was found to contain images with signs of duplication and manipulation in Figures 4 and 5.…”