Biomolecular Interaction, Anti-Cancer and Anti-Angiogenic Properties of Cobalt(III) Schiff Base Complexes

Abstract

Two cobalt(III) Schiff base complexes, trans-[Co(salen)(DA) 2 ](ClO 4 ) ( 1 ) and trans-[Co(salophen)(DA) 2 ](ClO 4 ) ( 2 ) (where salen: N,N’-bis(salicylidene)ethylenediamine, salopen: N,N’-bis(salicylidene)-1,2-phenylenediamine, DA: dodecylamine) were synthesised and characterised using various spectroscopic and analytical techniques. The binding affinity of both the complexes with CT-DNA was explored adopting UV-visible, fluorescence, circular dichroism spectroscopy and cyclic voltammetry techniques. The results revealed that both the complexes interacted with DNA via intercalation as well as notable groove binding. Protein (BSA) binding ability of these complexes was investigated by absorption and emission spectroscopy which indicate that these complexes engage in strong hydrophobic interaction with BSA. The mode of interaction between these complexes and CT-DNA/BSA was studied by molecular docking analysis. The in vitro cytotoxic property of the complexes was evaluated in A549 (human small cell lung carcinoma) and VERO (African green monkey kidney cells). The results revealed that the complexes affect viability of the cells. AO and EB staining and cell cycle analysis revealed that the mode of cell death is apoptosis. Both the complexes showed profound inhibition of angiogenesis as revealed in in-vivo chicken chorioallantoic membrane (CAM) assay. Of the two complexes, the complex 2 proved to be much more efficient in affecting the viability of lung cancer cells than complex 1 . These results indicate that the cobalt(III) Schiff base complexes in this study can be potentially used for cancer chemotherapy and as inhibitor of angiogenesis, in general, and lung cancer in particular, for which there is need for substantiation at the level of signalling mechanisms and gene expressions.

Preparation, Spectroscopic Characterization, Theoretical Investigations, and In Vitro Anticancer Activity of Cd(II), Ni(II), Zn(II), and Cu(II) Complexes of 4(3H)-Quinazolinone-Derived Schiff Base

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Schiff base metal complex as a potential therapeutic drug in medical science: A critical review

“…A good lead to narrow-spectrum antibiotics could be expected from such compounds. The study revealed the potential use of such metal complexes in cancer chemotherapy and as an inhibitor of angiogenesis [56]. Benzimidazole belongs to such a potent scaffold that possesses a wide variety of functionalizations and coordination modes.…”

Fragmentation Study, Dual Anti-Bactericidal and Anti-Viral Effects and Molecular Docking of Cobalt(III) Complexes

“…In the bioinorganic chemistry field, besides the strong research on active drugs containing essential metals including Fe [9], Zn [8,10], Cu [11], Co [12], and Mg [13], among others, metallodrugs based on those metal ions have not successfully advanced in clinical trials so far. Moreover, a great advantage of potential drugs containing an essential metal would be the lower levels of toxicity on healthy cells.…”