Biomolecular immobilization on conducting polymers for biosensing applications

Ahuja, Tarushee; Mir, Irfan Ahmad; Kumar, Devendra; Rajesh, Rajesh

doi:10.1016/j.biomaterials.2006.09.046

Cited by 463 publications

(234 citation statements)

References 209 publications

(205 reference statements)

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“…Although there have been a number of reviews on CPs with regard to biomedical applications (Rivers et al 2002;Zelikin et al 2002;Gizdavic-Nikolaidis et al 2004a,b;Schultze & Karabulut 2005;Abidian et al 2006;Ahuja et al 2007;Guimard et al 2007a,b), this review focuses solely on the various tissue engineering and drug-delivery applications. Moreover, this review accentuates the various surface functionalization techniques that can be used in order to modify the physico-chemical, electrical and mechanical properties of the CPs so as to improve their potential biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Applications of conducting polymers and their issues in biomedical engineering

Ravichandran

Sundarrajan

Venugopal

et al. 2010

J. R. Soc. Interface.

377

253

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Conducting polymers (CPs) have attracted much interest as suitable matrices of biomolecules and have been used to enhance the stability, speed and sensitivity of various biomedical devices. Moreover, CPs are inexpensive, easy to synthesize and versatile because their properties can be readily modulated by (i) surface functionalization techniques and (ii) the use of a wide range of molecules that can be entrapped or used as dopants. This paper discusses the various surface modifications of the CP that can be employed in order to impart physico-chemical and biological guidance cues that promote cell adhesion/proliferation at the polymer-tissue interface. This ability of the CP to induce various cellular mechanisms widens its applications in medical fields and bioengineering.

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Section: Introductionmentioning

confidence: 99%

Applications of conducting polymers and their issues in biomedical engineering

Ravichandran

Sundarrajan

Venugopal

et al. 2010

J. R. Soc. Interface.

377

253

View full text Add to dashboard Cite

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“…When the enzyme is embedded in the polymer membrane, it is important to preserve its native structure/function and to allow the diffusion of specific substrates into the polymeric membrane. These enzyme-polymer hybrid materials are of great interest to technology and medical applications [6] because they serve as very rapid, extremely selective detectors. Such detectors can be used for diagnostic purposes, for example.…”

Section: Immobilization Of Biomolecules On Polymer Membranesmentioning

confidence: 99%

“…Since the electronic device requires a certain threshold for detection, diffusion is not an optimal solution to transfer a signal from the enzyme to the electrode, due to the delay in registering the products and their low probability of arriving at the electrode. In this respect, conductive polymers can help improve the collection of the reaction products and therefore create a more detectable signal in the biosensor [6]. It may also be possible to use conformational changes, as done by enzymes, to improve the sensitivity of enzyme-polymer biosensors.…”

Section: Immobilization Of Biomolecules On Polymer Membranesmentioning

confidence: 99%

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Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

et al. 2011

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Today, demand exists for new systems that can meet the challenges of identifying biological entities rapidly and specifically in diagnostics, developing stable and multifunctional membranes, and engineering devices at the nanometer scale. In this respect, bio-decorated membranes combine the specificity and efficacy of biological entities, such as peptides, proteins, and DNA, with stability and the opportunity to chemically tailor the properties of polymeric membranes. A smart strategy that serves to fulfill biological criteria is required, whereby polymer membranes come to mimic biological membranes and do not disturb but rather enhance the functioning and activity of a biological entity. Different approaches have been developed, exemplified by either planar or vesicular membranes, allowing insertion inside the polymer membrane or anchoring via functionalization of the membrane surface. Inspired by nature, but incorporating the strength provided by chemical design, bio-decorated polymer membranes represent a novel concept with great potential in diagnostics and therapeutics.

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“…[2,3] For biosensor applications, different methods can be used to immobilize bioreceptors on the transducer surface, [4] like protein entrapment. This technique is not applicable for polyaniline because this polymer is synthesized under highly acidic solutions conditions (pH 0-2).…”

Section: Introductionmentioning

confidence: 99%

Grafting and characterization of protein on polyaniline surface for biosensor applications

Crombrugghe

Yunus

Bertrand

2008

Surface & Interface Analysis

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Polyaniline is an electroactive polymer, which presents attractive properties for its use as a transducer in electro-biosensors. Covalent binding of bioreceptors on the polymeric surface is necessary to perform selective and efficient bioanalyte detection. To achieve this goal, acrylic acid (AAc) is grafted on polyaniline films. Acrylic grafting on polyaniline introduces a new access to its biochemical functionalization. Carboxylic groups can be used for covalent immobilization of proteins, like ICHA antigen, by means of standard coupling agents (carbodiimide and succinimide). Surface analysis shows that there is a higher amount of antigen immobilized on the surface as compared with simple physical adsorption. The composition and structure of the modified surfaces are characterized by X-ray photoelectron spectroscopy (XPS) at each step of the process. Time-of-flight-secondary ion mass spectrometry (Tof-SIMS) is also used to analyze acrylic grafted polyaniline surfaces.

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Biomolecular immobilization on conducting polymers for biosensing applications

Cited by 463 publications

References 209 publications

Applications of conducting polymers and their issues in biomedical engineering

Applications of conducting polymers and their issues in biomedical engineering

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

Grafting and characterization of protein on polyaniline surface for biosensor applications

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