Biomolecular identification of allergenic pollen: a new perspective for aerobiological monitoring?

Longhi, Sara; Cristofori, Alessandro; Gatto, Pamela; Cristofolini, F.; Grando, S.; Gottardini, E.

doi:10.1016/s1081-1206(10)60268-2

Cited by 52 publications

(54 citation statements)

References 24 publications

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“…Until now, only a real-time PCR method based on the detection of random amplified polymorphic DNA-derived sequence was reported for identification of hazelnut allergenic pollen. [18] Another situation is within the detection of hazelnut food allergy, where a real-time PCR approach is a well established method. [26,27] Similar approaches based on real-time PCR have already been applied in many different fields to the detection and quantification from transgene constructs [28] to the allergens contained in foods.…”

Section: Resultsmentioning

confidence: 99%

“…This tool for evaluating of birch pollen allergens was used before by Ziarovsk a et al [17] Longhi et al [18] used real-time PCR as a rapid, accurate, and automated tool for the detection and quantification of airborne allergenic pollen taxa. Real-time PCR is also a useful tool for detection: gene expression [19] identification and quantification of pathogens or allergens, [20][21][22] and authentication of food.…”

Section: Introductionmentioning

confidence: 99%

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Variability ofCorylus Avellana, L. CorA and profilin pollen allergens expression

Ražná

Bežo

Nikolaieva

et al. 2014

Journal of Environmental Science and Health, Part B

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Corylus avellana is the source of inhalant allergies induced by hazel pollen as well as food allergies induced after ingestion of hazelnuts. In this study, real-time PCR approach was used to analyse expression of hazel pollen allergens on the molecular level. Relative quantity of hazelnut allergens Corylus avellana, L. CorA and Corylus avellana, L. pollen profiling in samples from different Ukraine areas were determining and comparing. Differences among the levels of both analysed allergen transcripts were found for hazel CorA and profillin. In both cases, the expression within the urbanized growth conditions was higher when compared to the sample from village area. The average expression for CorA was 0.84 times higher than for profilin and the results are very variable depending on the place of growth. Expression levels here were within the range of 2.957 up to the 52.936. Profilin expression was the highest in the sample from the polluted place of growth-cement plant area with the value of 52 times higher when compared to the sample from the village area. In this study, comparison of expression levels of hazel CorA and profiling pollen allergens was performed for the first time. Real-time PCR assay developed in this study proved the sensitivity for detection of the changes of the hazel pollen allergens expression levels and could benefit labs by fast and reproducible detection method of these allergens.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variability ofCorylus Avellana, L. CorA and profilin pollen allergens expression

Ražná

Bežo

Nikolaieva

et al. 2014

Journal of Environmental Science and Health, Part B

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show abstract

“…Early studies typically relied on either direct Sanger sequencing of purified PCR amplicons (Longhi et al 2009;Wilson et al 2010), for singlespecies barcoding, or else, for multi-species samples, Sanger sequencing of amplicon clones randomly selected from PCR products (Galimberti et al 2014;Bruni et al 2015). The latter has mostly been superseded by HTS approaches for DNA metabarcoding.…”

Section: Component 4: Sequencing Methodsologies and Bioinformatics Pipmentioning

confidence: 99%

Pollen DNA barcoding: current applications and future prospects

Bell

Vere

Keller

et al. 2016

Genome

184

170

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Identification of the species origin of pollen has many applications, including assessment of plant-pollinator networks, reconstruction of ancient plant communities, product authentication, allergen monitoring, and forensics. Such applications, however, have previously been limited by microscopy-based identification of pollen, which is slow, has low taxonomic resolution, and has few expert practitioners. One alternative is pollen DNA barcoding, which could overcome these issues. Recent studies demonstrate that both chloroplast and nuclear barcoding markers can be amplified from pollen. These recent validations of pollen metabarcoding indicate that now is the time for researchers in various fields to consider applying these methods to their research programs. In this paper, we review the nascent field of pollen DNA barcoding and discuss potential new applications of this technology, highlighting existing limitations and future research developments that will improve its utility in a wide range of applications.Key words: DNA metabarcoding, metagenomics, pollen, palynology, high-throughput sequencing, next-generation sequencing.Résumé : L'identification de l'espèce à l'origine d'un pollen se prête à de nombreuses applications dont la description des réseaux plante-pollinisateur, la reconstruction de communautés de plantes anciennes, l'authentification de produits, la surveillance des allergènes et les enquêtes médicolégales. Cependant, ces applications ont précédemment été limitées à l'identification du pollen par examen microscopique, un processus lent, à faible résolution taxonomique et qui compte peu de praticiens experts. Une alternative est l'identification du pollen par le recours aux codes à barres de l'ADN, une avenue qui permettrait de surmonter plusieurs de ces limitations. De récentes études ont montré qu'il était possible d'amplifier les marqueurs de codage tant chloroplastiques que nucléaires à partir du pollen. Ces récentes validations du métacodage à barres chez le pollen indiquent qu'il est maintenant opportun pour les chercheurs dans divers domaines de considérer l'emploi de ces méthodes dans leurs programmes de recherche. Dans cet article, les auteurs passent en revue le domaine naissant du codage à barres du pollen et discutent des nouvelles applications potentielles de cette technologie en mettant en lumière les limitations existantes ainsi que de futurs développements qui pourraient accroître son utilité dans un grand nombre d'applications. [Traduit par la Rédaction] Mots-clés : métacodage à barres, métagénomique, pollen, palynologie, séquençage à haut débit, séquençage de nouvelle génération.

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“…The assessment of allergen content in the air is feasible using antibody-based methods (18,19,30) or the biomolecular identification of pollen genomes (31). However, sophisticated methods are required which may not account for all of the pollen species in the ambient air, and individual measurements are not feasible.…”

Section: Time Of Onset Of the Allergy Seasonmentioning

confidence: 99%

MACVIA-ARIA Sentinel NetworK for allergic rhinitis (MASK-rhinitis): the new generation guideline implementation

Bousquet

Schünemann

Fonseca

et al. 2015

Allergy

174

172

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Biomolecular identification of allergenic pollen: a new perspective for aerobiological monitoring?

Cited by 52 publications

References 24 publications

Variability ofCorylus Avellana, L. CorA and profilin pollen allergens expression

Variability ofCorylus Avellana, L. CorA and profilin pollen allergens expression

Pollen DNA barcoding: current applications and future prospects

MACVIA-ARIA Sentinel NetworK for allergic rhinitis (MASK-rhinitis): the new generation guideline implementation

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