Biomolecular coronas provide the biological identity of nanosized materials

Monopoli, Marco P.; Åberg, Christoffer; Salvati, Anna; Dawson, Kenneth A.

doi:10.1038/nnano.2012.207

Cited by 2,357 publications

(2,078 citation statements)

References 110 publications

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“…Numerous studies have shown that it is the biophysicochemical properties of the biomolecular corona, instead of properties of the pristine NPs, that determine the biological identity of the NPs in interacting with the biological system. 4 As a counterpart of the protein corona formed on NPs entering the blood vessels, the PS corona is acquired immediately and inevitably after NPs enter the respiratory system. Once the PS corona is formed on the NP surface, the biophysicochemical properties of the corona determine the further nano-bio interactions of the NPs, such as their bioavailability, biodistribution, retention, translocation, and clearance.…”

Section: Resultsmentioning

confidence: 99%

“…50 Third, the PS corona coated on the pristine NP obviously increases the phosphate group density on the NP surface. Because the phosphate group plays a crucial role in the biorecognition of NPs, 4 the PS corona may lead to enhanced cellular uptake of the NPs. 28,30,49 Due to differential molecular conformations of the PS corona formed on the hydrophilic and hydrophobic NPs (Figures 2−5), the phosphate group on the Ag-NP shows a larger density than that on the PST-NP (Table 1), thus indicating an easier cellular uptake of the hydrophilic NPs than the hydrophobic NPs.…”

Section: Resultsmentioning

confidence: 99%

“…2−4 Biophysicochemical properties of this corona, instead of the pristine NPs, determines the biological identity of the NPs when further interacting with the biological system. 4 Numerous studies have shown that the biomolecular corona alters the biodistribution, bioreactivity, and biopersistence of NPs, 1,5 thus resulting in differential cellular uptake and cytotoxicity of the NPs. 6−9 To date, a majority of studies in the biomolecular corona focuses on the protein corona.…”

Section: U Nderstanding Interactions Between Nanoparticlesmentioning

confidence: 99%

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Unveiling the Molecular Structure of Pulmonary Surfactant Corona on Nanoparticles

et al. 2017

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The growing risk of human exposure to airborne nanoparticles (NPs) causes a general concern on the biosafety of nanotechnology. Inhaled NPs can deposit in the deep lung at which they interact with the pulmonary surfactant (PS). Despite the increasing study of nano-bio interactions, detailed molecular mechanisms by which inhaled NPs interact with the natural PS system remain unclear. Using coarse-grained molecular dynamics simulation, we studied the interaction between NPs and the PS system in the alveolar fluid. It was found that regardless of different physicochemical properties, upon contacting the PS, both silver and polystyrene NPs are immediately coated with a biomolecular corona that consists of both lipids and proteins. Structure and molecular conformation of the PS corona depend on the hydrophobicity of the pristine NPs. Quantitative analysis revealed that lipid composition of the corona formed on different NPs is relatively conserved and is similar to that of the bulk phase PS. However, relative abundance of the surfactant-associated proteins, SP-A, SP-B, and SP-C, is notably affected by the hydrophobicity of the NP. The PS corona provides the NPs with a physicochemical barrier against the environment, equalizes the hydrophobicity of the pristine NPs, and may enhance biorecognition of the NPs. These modifications in physicochemical properties may play a crucial role in affecting the biological identity of the NPs and hence alter their subsequent interactions with cells and other biological entities. Our results suggest that all studies of inhalation nanotoxicology or NP-based pulmonary drug delivery should consider the influence of the PS corona.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: U Nderstanding Interactions Between Nanoparticlesmentioning

confidence: 99%

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Unveiling the Molecular Structure of Pulmonary Surfactant Corona on Nanoparticles

et al. 2017

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“…[14][15][16] However, NPs dispersed in a biological fluid are rapidly covered by biomolecules, such as proteins and lipids, forming a biomolecular 'corona' that effectively screens the bare NP surface. [17][18][19][20][21][22] When cells are exposed to NPs it is therefore, in realistic circumstances, typically not the bare NP surface that interacts with the cell but the NP-biomolecular corona complex. [23][24][25] Consequently, it becomes interesting to correlate NP uptake not to properties of the bare NP, but to properties of the NP-biomolecular corona complex.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle Adhesion to the Cell Membrane and Its Effect on Nanoparticle Uptake Efficiency

et al. 2013

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ABSTRACT:The interactions between nano-sized particles and living systems are commonly mediated by what adsorbs to the nanoparticle in the biological environment, its "biomolecular corona", rather than the pristine surface. Here we characterise the adhesion towards the cell membrane of nanoparticles of different material and size, and study how this is modulated by the presence or absence of a corona on the nanoparticle surface. The results are corroborated with adsorption to simple model supported lipid bilayers using a quartz crystal microbalance. We conclude that the adsorption of proteins on the nanoparticle surface strongly reduces nanoparticle adhesion in comparison to what is observed for the bare material. Nanoparticle uptake is described as a two-step process, where the nanoparticles initially adhere to the cell membrane and subsequently are internalised by the cells via energy-dependent pathways. The lowered adhesion in the presence of proteins thereby causes a concomitant decrease in nanoparticle uptake efficiency. The presence of a biomolecular corona may confer specific interactions between the nanoparticle-corona complex and the cell surface, including triggering of regulated cell uptake. An important effect of the corona is, however, a reduction in the purely unspecific interactions between the bare material and the cell membrane, which in itself disregarding specific interactions, causes a decrease in cellular uptake. We suggest that future nanoparticle-cell studies include, together with characterisation of size, charge and dispersion stability, an evaluation of the adhesion properties of the material to relevant membranes.

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“…33,34 By changing nanoparticle properties and design, the profile of proteins adsorbed to their surfaces from the surrounding environment, such as for instance blood serum, is also changed. 35,36 It has been hypothesized that this layer (corona) of proteins on the nanoparticle surface affects much of the interactions with cells 37,38 and thus properties of the bare nanoparticle surface, such as charge, are less important for cellular interactions. Indeed initial evidence suggests that the capacity of nanoparticles to cross the BBB is connected to the nature of the proteins adsorbed on their surface, such as, for instance, apolipoproteins.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle accumulation and transcytosis in brain endothelial cell layers

et al. 2013

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The Blood-Brain Barrier (BBB) is a selective barrier, which controls and limits access to the central nervous system (CNS). The selectivity of the BBB relies on specialized characteristics of the endothelial cells that line the microvasculature, including the expression of intercellular tight junctions, which limit paracellular permeability. Several reports suggest that nanoparticles have a unique capacity to cross the BBB. However, direct evidence of nanoparticle transcytosis is difficult to obtain, and we found that typical transport studies present several limitations when applied to nanoparticles. In order to investigate the capacity of nanoparticles to access and transport across the BBB, several different nanomaterials, including silica, titania and albumin-or transferrinconjugated gold nanoparticles of different sizes, were exposed to a human in vitro BBB model of endothelial hCMEC/D3 cells. Extensive transmission electron microscopy imaging was applied in order to describe nanoparticle endocytosis and typical intracellular localisation, as well as to look for evidence of eventual transcytosis. Our results show that all of the nanoparticles were internalised, to different extents, by the BBB model and accumulated along the endo-lysosomal pathway. Rare events suggestive of nanoparticle transcytosis were also observed for several of the tested materials.

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Biomolecular coronas provide the biological identity of nanosized materials

Abstract: Publication informationNature nanotechnology, 7 (12): 779-786Publisher Nature Publishing Group Item record/more information

Cited by 2,357 publications

References 110 publications

Unveiling the Molecular Structure of Pulmonary Surfactant Corona on Nanoparticles

Unveiling the Molecular Structure of Pulmonary Surfactant Corona on Nanoparticles

Nanoparticle Adhesion to the Cell Membrane and Its Effect on Nanoparticle Uptake Efficiency

Nanoparticle accumulation and transcytosis in brain endothelial cell layers

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