Biomolecular alterations in acute traumatic brain injury (TBI) using Fourier transform infrared (FTIR) imaging spectroscopy

Rakib, Fazle; Al‐Saad, Khalid; Ahmed, Tariq; Ullah, Ehsan; Barreto, George E.; Ashraf, Ghulam Md; Ali, Maha

doi:10.1016/j.saa.2020.119189

Cited by 13 publications

(29 citation statements)

References 59 publications

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“…A FTIR micro-spectrometer (Agilent Cary 620, Cary, NC, USA) was used to record the FTIR spectra and maps within the range of 4000-700 cm −1 (spectral resolution 4 cm −1 ). More detailed instrument configurations and parameters used for this study were conducted as in our previous studies [15,16,25,41,42]. The FTIR spectra from two identical anatomical brain regions in the cortex and corpus callosum of different animals from each group were extracted from the FTIR images.…”

Section: Fourier Transform Infrared Imaging (Ftir) Studymentioning

confidence: 99%

“…Lipid molecular and structural alterations, lipid/protein band ratios (that provide facts about the protein-lipid asymmetry and environmental changes, which lead to devastating effect on the cellular function), vector normalization, and second derivatives of raw spectra were used and calculated according to the details explained in our previous studies [15]. Relative quantification of the protein content and the distribution of amide I components were determined according to our previous studies [15,16,25,41,42]. The mean was generated for each animal group along with their standard deviation (significant level p < 0.0001).…”

Section: Fourier Transform Infrared Imaging (Ftir) Studymentioning

confidence: 99%

“…Tragically, the mainstream mTBI brain injuries go undiagnosed. The non-appearance of the injuries on traditional imaging studies and the unintentional perception of an expected appearing and performing individuals are the two challenging factors to diagnose this injury [ 41 ]. In our previous study, the molecular changes in different brain regions due to stroke and mTBI 3 h after the injury were investigated by FTIR imaging [ 40 , 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

“…The non-appearance of the injuries on traditional imaging studies and the unintentional perception of an expected appearing and performing individuals are the two challenging factors to diagnose this injury [ 41 ]. In our previous study, the molecular changes in different brain regions due to stroke and mTBI 3 h after the injury were investigated by FTIR imaging [ 40 , 41 , 42 ]. In this paper, we aimed to investigate the imbalance in biochemical components in the cortex (Co) and corpus callosum (CC) of mTBI after 24 h from the injury by FTIR imaging for their greater implication on accuracy and sensitivity as a research tool.…”

Section: Introductionmentioning

confidence: 99%

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Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury

et al. 2021

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Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution.

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Section: Fourier Transform Infrared Imaging (Ftir) Studymentioning

confidence: 99%

Section: Fourier Transform Infrared Imaging (Ftir) Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury

et al. 2021

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“…FTIR spectroscopy has been previously used to assess brain tissue status in response to various conditions, including radiation 16 , Alzheimer’s disease 17 , brain tumors 18 , multiple sclerosis 19 , traumatic axonal injury (TAI) 20 , diffuse axonal injury (DAI) 21 , 22 , ischemic brain injury 23 , and intracerebral hemorrhage 24 . Previous FTIR Spectroscopy and Imaging studies on brain tissues by our group include investigation of the effects of whole body ionizing radiation on rat brain tissue 16 and homogenate membrane 25 , vitamin A deficiency (VAD) on hippocampal region in rat brain 26 , pentylenetetrazol-induced seizures on homogenate rat brain tissues 27 , ischemic stroke on rat brain 28 , as well as general biochemical analysis of different rat brain regions in healthy 29 and 3 h post-TBI 30 animals.…”

Section: Introductionmentioning

confidence: 99%

Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury

Ustaoğlu

Ali

Rakib

et al. 2021

Sci Rep

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Traumatic brain injury (TBI) is the main cause of disability and mortality in individuals under the age of 45 years. Elucidation of the molecular and structural alterations in brain tissue due to TBI is crucial to understand secondary and long-term effects after traumatic brain injury, and to develop and apply the correct therapies. In the current study, the molecular effects of TBI were investigated in rat brain at 24 h and 1 month after the injury to determine acute and chronic effects, respectively by Fourier transform infrared imaging. This study reports the time-dependent contextual and structural effects of TBI on hippocampal brain tissue. A mild form of TBI was induced in 11-week old male Sprague Dawley rats by weight drop. Band area and intensity ratios, band frequency and bandwidth values of specific spectral bands showed that TBI causes significant structural and contextual global changes including decrease in carbonyl content, unsaturated lipid content, lipid acyl chain length, membrane lipid order, total protein content, lipid/protein ratio, besides increase in membrane fluidity with an altered protein secondary structure and metabolic activity in hippocampus 24 h after injury. However, improvement and/or recovery effects in these parameters were observed at one month after TBI.

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A Literature Review of Traumatic Brain Injury Biomarkers

et al. 2022

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Research into TBI biomarkers has accelerated rapidly in the past decade owing to the heterogeneous nature of TBI pathologies and management, which pose challenges to TBI evaluation, management, and prognosis. TBI biomarker proteins resulting from axonal, neuronal, or glial cell injuries are widely used and have been extensively studied. However, they might not pass the blood-brain barrier with sufficient amounts to be detected in peripheral blood specimens, and further might not be detectable in the cerebrospinal fluid owing to flow limitations triggered by the injury itself. Despite the advances in TBI research, there is an unmet clinical need to develop and identify novel TBI biomarkers that entirely correlate with TBI pathologies on the molecular level, including mild TBI, and further enable physicians to predict patient outcomes and allow researchers to test neuroprotective agents to limit the extents of injury. Although the extracellular vesicles have been identified and studied long ago, they have recently been revisited and repurposed as potential TBI biomarkers that overcome the many limitations of the traditional blood and CSF assays. Animal and human experiments demonstrated the accuracy of several types of exosomes and miRNAs in detecting mild, moderate, and severe TBI. In this paper, we provide a comprehensive review of the traditional TBI biomarkers that are helpful in clinical practice. Also, we highlight the emerging roles of exosomes and miRNA being the promising candidates under investigation of current research.

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Biomolecular alterations in acute traumatic brain injury (TBI) using Fourier transform infrared (FTIR) imaging spectroscopy

Cited by 13 publications

References 59 publications

Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury

Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury

Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury

A Literature Review of Traumatic Brain Injury Biomarkers

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