Biomineralization of varied calcium carbonate crystals by the synergistic effect of silk fibroin/magnesium ions in a microbial system

Zhu, Wenkun; Shi, Peiheng; Li, Yi; Duan, Tao; Yu, Yang; Li, Xianyan

doi:10.1039/c8ce00099a

Cited by 33 publications

(21 citation statements)

References 40 publications

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“…Figure 1a shows a representative scanning electron microscopy (SEM) image of SrCO 3 particles which have a slightly spherical shape, obtained after a mineralization time of 6 h. Figure 1b shows dumbbell-like SrCO 3 obtained after a mineralization time of 12 h. Interestingly, with the increase of the mineralization time, the dumbbell-like SrCO 3 turned into popcorn-like SrCO 3 via the process of dissolution and recrystallization, see Figure 1c. By magnifying Figure 1c, we can clearly see that there are many voids left by the death of the bacteria on the surface of the SrCO 3 crystal, indicating that the microorganism acts as a nucleation site for SrCO 3 crystallization, which is consistent with the experimental results of Chen et al [24,25,26]. The change in the surface morphology could reflect the dissolution and recrystallization process of nano-spherical SrCO 3 .…”

Section: Resultssupporting

confidence: 87%

“…The concentration of nano-MMT has a significant influence on the formation of SrCO 3 crystals with unique morphologies because nano-MMT determined the supersaturation of SrCO 3 in mineralized systems. The formation of flower-like SrCO 3 crystals may be attributed to the high concentration of nano-MMT which led to excessive accumulation of rod-like SrCO 3 crystals around the monolayer nano-MMT [26].…”

Section: Resultsmentioning

confidence: 99%

“…With the mineralization time increasing, the nanostructured spherical morphology did not change significantly in the aggregated state and crystallization. Therefore, the ordered arrangement of spherical SrCO 3 nanocrystals aggregated primary building blocks (rod-like SrCO 3 ) formed via the van der Waals force and electrostatic interaction, and then gradually formed cross-shaped SrCO 3 aggregates via the dissolution and recrystallization process under the attraction of nano-MMT [25,26]. With the increasing concentration of nano-MMT, a second nucleation process occurs on the as-prepared cross-shaped surface.…”

Section: Resultsmentioning

confidence: 99%

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Nano-Montmorillonite Regulated Crystallization of Hierarchical Strontium Carbonate in a Microbial Mineralization System

et al. 2019

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In this paper, nano-montmorillonite (nano-MMT) was introduced into the microbial mineralization system of strontium carbonate (SrCO3). By changing the nano-MMT concentration and the mineralization time, the mechanism of mineralization was studied. SrCO3 superstructures with complex forms were acquired in the presence of nano-MMT as a crystal growth regulator. At low concentrations of nano-MMT, a cross-shaped SrCO3 superstructure was obtained. As the concentration increased, flower-like SrCO3 crystals formed via the dissolution and recrystallization processes. An emerging self-assembly process and crystal polymerization mechanism have been proposed by forming complex flower-like SrCO3 superstructures in high concentrations of nano-MMT. The above research indicated that unique bionic synthesis strategies in microbial systems could not only provide a useful route for the production of inorganic or inorganic/organic composites with a novel morphology and unique structure but also provide new ideas for the treatment of radionuclides.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Nano-Montmorillonite Regulated Crystallization of Hierarchical Strontium Carbonate in a Microbial Mineralization System

et al. 2019

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“…In particular, SF NPs with a large specific surface area exhibit outstanding aqueous stability and can serve as the sacrificial template or reductant for assembly of nanostructures. For instance, crystallization of CaCO 3 and MnO 2 can be achieved on the skeleton of SF via biomineralization-inspired metal reduction 30 , 31 . However, until now, no studies related to the synthesis of novel nanocatalyst through SF-mediated bioinspired crystallization have been conducted.…”

Section: Introductionmentioning

confidence: 99%

Facile engineering of silk fibroin capped AuPt bimetallic nanozyme responsive to tumor microenvironmental factors for enhanced nanocatalytic therapy

Yang¹,

Fu²,

Li³

et al. 2021

Theranostics

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Background: Reactive oxygen species (ROS), as a category of highly reactive molecules, are attractive for eliminating tumor cells in situ . However, the intrinsic tumor microenvironment (TME) always compromises treatment efficacy. In another aspect, silk fibroin (SF), as a category of natural biomacromolecules, is highly promising for synthesis of metallic nanocrystals via biomineralization. Methods: As a proof-of-concept study, AuPt bimetallic nanozyme derived from bioinspired crystallization of chloroauric acid and chloroplatinic acid was facilely developed in the presence of silk fibroin (SF). Antitumor effects caused by the as-synthesized AuPt@SF (APS) nanozyme were demonstrated in 4T1 tumor cells in vitro and xenograft tumor models in vivo . Results: APS nanozyme can decompose glucose to constantly supply H 2 O 2 and deplete intracellular glutathione (GSH). APS nanozyme can simultaneously convert adsorbed O 2 and endogenic H 2 O 2 into superoxide radicals ( • O 2 - ) and hydroxyl radical ( • OH), respectively, upon highly efficient catalytic reaction. Subsequently, these cytotoxic ROS cause irreversible damage to the cell membrane, nucleic acid and mitochondria of tumors. Upon fluorescence/photoacoustic (FL/PA)-imaging guidance, remarkable tumor damage based on the current nanoplatform was confirmed in vivo . Conclusion: The objective of our investigation is to supply more useful insights on the development of SF-based nanocatalysts, which are specifically responsive to TME for extremely efficient tumor theranostics.

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“…Previous reports indicated that biomacromolecules were closely related to bioinspired mineralization process. For instance, the mineralization of CaCO 3 can be induced and regulated by SF-based nanostructures through an oriented crystallization process 26, 27. Therefore, controllable growth of inorganic substances on the skeleton of SF could be achieved by biomineralization-inspired metal reduction, rendering new possibilities of therapeutic-related functionalization of SF matrix.…”

Section: Introductionmentioning

confidence: 99%

Biomineralization-inspired Crystallization of Manganese Oxide on Silk Fibroin Nanoparticles for in vivo MR/fluorescence Imaging-assisted Tri-modal Therapy of Cancer

et al. 2019

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Regenerated silk fibroin (SF) is a type of natural biomacromolecules with outstanding biocompatibility and biodegradability. However, stimulus-responsive SF-based nanocomplex has seldom been reported for application in tumor diagnosis and therapy.Methods: As a proof-of-concept study, a multifunctional SF@MnO2 nanoparticle-based platform was strategically synthesized using SF as a reductant and a template via a biomineralization-inspired crystallization process in an extremely facile way. Because of their mesoporous structure and abundant amino and carboxyl terminal residues, SF@MnO2 nanoparticles were co-loaded with a photodynamic agent indocyanine green (ICG) and a chemotherapeutic drug doxorubicin (DOX) to form a SF@MnO2/ICG/DOX (SMID) nanocomplex.Results: The obtained product was highly reactive with endogenous hydrogen peroxide (H2O2) in tumor microenvironment, which was decomposed into O2 to enhance tumor-specific photodynamic therapy (PDT). Moreover, SMID nanocomplex produced a strong and stable photothermal effect upon near-infrared (NIR) irradiation for photothermal therapy (PTT) owing to the distinct photothermal response of SF@MnO2 and stably conjugated ICG. The concurrent NIR fluorescence and magnetic resonance (MR) imaging in vivo both indicated effective tumor-specific enrichment of SMID nanoparticles via enhanced permeability and retention (EPR) effect. Animal studies further verified that SMID nanoparticles remarkably improved tumor inhibitive efficacy through combination PTT/PDT/chemotherapy with minimal systemic toxicity or adverse effect.Conclusion: This study demonstrated the promising potential of SF-based nanomaterial to address some of the key challenges in cancer therapy due to unfavorable tumor microenvironment for drug delivery.

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Biomineralization of varied calcium carbonate crystals by the synergistic effect of silk fibroin/magnesium ions in a microbial system

Abstract: The influence of silk fibroin (SF) and magnesium ions (Mg2+) on calcium carbonate (CaCO3) bio-mineralization has been investigated.

Cited by 33 publications

References 40 publications

Nano-Montmorillonite Regulated Crystallization of Hierarchical Strontium Carbonate in a Microbial Mineralization System

Nano-Montmorillonite Regulated Crystallization of Hierarchical Strontium Carbonate in a Microbial Mineralization System

Facile engineering of silk fibroin capped AuPt bimetallic nanozyme responsive to tumor microenvironmental factors for enhanced nanocatalytic therapy

Biomineralization-inspired Crystallization of Manganese Oxide on Silk Fibroin Nanoparticles for in vivo MR/fluorescence Imaging-assisted Tri-modal Therapy of Cancer

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