Biomineralization of Poorly Crystalline Fe(III) Oxides by Dissimilatory Metal Reducing Bacteria (DMRB)

Zachara, John M.; Kukkadapu, Ravi; Fredrickson, James K.; Gorby, Yuri A.; Smith, Steven C.

doi:10.1080/01490450252864271

Cited by 347 publications

(448 citation statements)

References 65 publications

(119 reference statements)

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“…strain SW2 (31). This type of mineral transformation is suggested to result from the adsorption of Fe(II) onto ferric (hydr)oxide, promoting its transformation to the thermodynamically more stable goethite (61). Elemental analysis of both types of precipitates using EDS gives signals only for iron and oxygen, with the atomic ratio (O/Fe) of 1.4 Ϯ 0.1.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of a Genetically Tractable Photoautotrophic Fe(II)-Oxidizing Bacterium, Rhodopseudomonas palustris Strain TIE-1

Jiao

Kappler

Croal

et al. 2005

Appl Environ Microbiol

192

205

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We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H 2 , or thiosulfate as the electron donor and photoheterotrophically with a variety of organic carbon sources. TIE-1 also grows chemoheterotrophically in the dark. This isolate appears to be a new strain of the purple nonsulfur bacterial species Rhodopseudomonas palustris, based on physiological and phylogenetic analysis. Fe(II) oxidation is optimal at pH 6.5 to 6.9. The mineral products of Fe(II) oxidation are pH dependent: below pH 7.0 goethite (␣-FeOOH) forms, and above pH 7.2 magnetite (Fe 3 O 4 ) forms. TIE-1 forms colonies on agar plates and is sensitive to a variety of antibiotics. A hyperactive mariner transposon is capable of random insertion into the chromosome with a transposition frequency of ϳ10 ؊5 . To identify components involved in phototrophic Fe(II) oxidation, mutants of TIE-1 were generated by transposon mutagenesis and screened for defects in Fe(II) oxidation in a cell suspension assay. Among approximately 12,000 mutants screened, 6 were identified that are specifically impaired in Fe(II) oxidation. Five of these mutants have independent disruptions in a gene that is predicted to encode an integral membrane protein that appears to be part of an ABC transport system; the sixth mutant has an insertion in a gene that is a homolog of CobS, an enzyme involved in cobalamin (vitamin B 12 ) biosynthesis.

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Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of a Genetically Tractable Photoautotrophic Fe(II)-Oxidizing Bacterium, Rhodopseudomonas palustris Strain TIE-1

Jiao

Kappler

Croal

et al. 2005

Appl Environ Microbiol

192

205

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“…The reason for the observed differences in electron transfer to ferrihydrite and anode surfaces is not clear. Formation of biominerals, such as magnetite (Fe 3 O 4 ), during ferrihydrite reduction, affects the rate of electron transfer reactions [42], and may partially explain the differences in electron transfer rates to ferrihydrite compared to anode surfaces.…”

Section: Fe(iii) Reduction and Current Generation By Pili And Flagellmentioning

confidence: 99%

The Role of Shewanella oneidensis MR‐1 Outer Surface Structures in Extracellular Electron Transfer

et al. 2010

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The ability of the metal reducer Shewanella oneidensis MR-1 to generate electricity in microbial fuel cells (MFCs) depends on the activity of a predicted type IV prepilin peptidase; PilD. Analysis of an S. oneidensis MR-1 pilD mutant indicated that it was deficient in pili production (Msh and type IV) and type II secretion (T2S). The requirement for T2S in metal reduction has been previously identified, but the role of pili remains largely unexplored. To define the role of type IV or Msh pili in electron transfer, mutants that lack one or both pilus biogenesis systems were generated and analyzed; a mutant that lacked flagella was also constructed and tested. All mutants were able to reduce insoluble Fe(III) and to generate current in MFCs, in contrast to the T2S mutant that is deficient in both processes. Our results show that loss of metal reduction in a PilD mutant is due to a T2S deficiency, and therefore the absence of c cytochromes from the outer surface of MR-1 cells, and not the loss of pili or flagella. Furthermore, MR-1 mutants deficient in type IV pili or flagella generated more current than the wild type, even though extracellular riboflavin levels were similar in all strains. This enhanced current generating ability is in contrast to a mutant that lacks the outer membrane c cytochromes, MtrC and OmcA. This mutant generated significantly less current than the wild type in an MFC and was unable to reduce Fe(III). These results indicated that although nanofilaments and soluble mediators may play a role in electron transfer, surface exposure of outer membrane c cytochromes was the determining factor in extracellular electron transfer in S. oneidensis MR-1.

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“…Fe-oxides and iron-bearing clay minerals, which are widely distributed in soils and sediments, represent a large reserve of Fe(III) for DMRB (Kostka et al, 2002;Zachara et al, 2002;Kappler and Straub, 2005). This includes contaminated US-DOE sites where cleanup efforts are underway to immobilize uranium as uraninite (N'Guessan et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Products of abiotic U(VI) reduction by biogenic magnetite and vivianite

Veeramani

Alessi

Suvorova

et al. 2011

Geochimica et Cosmochimica Acta

135

122

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Reductive immobilization of uranium by the stimulation of dissimilatory metal-reducing bacteria (DMRB) has been investigated as a remediation strategy for subsurface U(VI) contamination. In those environments, DMRB may utilize a variety of electron acceptors, such as ferric iron which can lead to the formation of reactive biogenic Fe(II) phases. These biogenic phases could potentially mediate abiotic U(VI) reduction. In this work, the DMRB Shewanella putrefaciens strain CN32 was used to synthesize two biogenic Fe(II)-bearing minerals: magnetite (a mixed Fe(II)-Fe(III) oxide) and vivianite (an Fe(II)-phosphate). Analysis of abiotic redox interactions between these biogenic minerals and U(VI) showed that both biogenic minerals reduced U(VI) completely. XAS analysis indicates significant differences in speciation of the reduced uranium after reaction with the two biogenic Fe(II)-bearing minerals. While biogenic magnetite favored the formation of structurally ordered, crystalline UO 2 , biogenic vivianite led to the formation of a monomeric U(IV) species lacking U-U associations in the corresponding EXAFS spectrum. To investigate the role of phosphate in the formation of monomeric U(IV) such as sorbed U(IV) species complexed by mineral surfaces, versus a U(IV) mineral, uranium was reduced by biogenic magnetite that was pre-sorbed with phosphate. XAS analysis of this sample also revealed the formation of monomeric U(IV) species suggesting that the presence of phosphate hinders formation of UO 2 . This work shows that U(VI) reduction products formed during in situ biostimulation can be influenced by the mineralogical and geochemical composition of the surrounding environment, as well as by the interfacial solute-solid chemistry of the solid-phase reductant.

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Biomineralization of Poorly Crystalline Fe(III) Oxides by Dissimilatory Metal Reducing Bacteria (DMRB)

Cited by 347 publications

References 65 publications

Isolation and Characterization of a Genetically Tractable Photoautotrophic Fe(II)-Oxidizing Bacterium, Rhodopseudomonas palustris Strain TIE-1

Isolation and Characterization of a Genetically Tractable Photoautotrophic Fe(II)-Oxidizing Bacterium, Rhodopseudomonas palustris Strain TIE-1

The Role of Shewanella oneidensis MR‐1 Outer Surface Structures in Extracellular Electron Transfer

Products of abiotic U(VI) reduction by biogenic magnetite and vivianite

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