Biomineralization-inspired sandwich dentin desensitization strategy based on multifunctional nanocomposite with yolk–shell structure

Yi, Luyao; Wu, Hongling; Xu, Yue; Yu, Jianming; Zhao, Yaning; Yang, Hongye; Huang, Cui

doi:10.1039/d2nr04993g

Cited by 8 publications

(6 citation statements)

References 75 publications

(118 reference statements)

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“…The single layer of type-I collagen fibrils was reconstituted according to our previous study. 33 The collagen fibrils showed periodic banding patterns of approximately 67 nm (Figure S1). After incubation in mineralization precursor solutions (PAA-ACP or PAA-ASP) for 2 and 5 days, transmission electron microscopy (TEM) images showed that the electron density of collagen fibrils gradually increased (Figure S2), revealing the preliminary evidence of intrafibrillar minerals.…”

Section: Resultsmentioning

confidence: 99%

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Janus Membrane with Intrafibrillarly Strontium-Apatite-Mineralized Collagen for Guided Bone Regeneration

Zhao,

Sun,

et al. 2024

ACS Nano

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Commercial collagen membranes face difficulty in guided bone regeneration (GBR) due to the absence of hierarchical structural design, effective interface management, and diverse bioactivity. Herein, a Janus membrane called SrJM is developed that consists of a porous collagen face to enhance osteogenic function and a dense face to maintain barrier function. Specifically, biomimetic intrafibrillar mineralization of collagen with strontium apatite is realized by liquid precursors of amorphous strontium phosphate. Polycaprolactone methacryloyl is further integrated on one side of the collagen as a dense face, which endows SrJM with mechanical support and a prolonged lifespan. In vitro experiments demonstrate that the dense face of SrJM acts as a strong barrier against fibroblasts, while the porous face significantly promotes cell adhesion and osteogenic differentiation through activation of calciumsensitive receptor/integrin/Wnt signaling pathways. Meanwhile, SrJM effectively enhances osteogenesis and angiogenesis by recruiting stem cells and modulating osteoimmune response, thus creating an ideal microenvironment for bone regeneration.In vivo studies verify that the bone defect region guided by SrJM is completely repaired by newly formed vascularized bone. Overall, the outstanding performance of SrJM supports its ongoing development as a multifunctional GBR membrane, and this study provides a versatile strategy of fabricating collagen-based biomaterials for hard tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

“…The single layer of type-I collagen fibrils was reconstituted according to our previous study . The collagen fibrils showed periodic banding patterns of approximately 67 nm (Figure S1).…”

Section: Results and Discussionmentioning

confidence: 99%

Janus Membrane with Intrafibrillarly Strontium-Apatite-Mineralized Collagen for Guided Bone Regeneration

Zhao,

Sun,

et al. 2024

ACS Nano

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“…The isolation and culture of HDPSCs were conducted according to previous studies. [48,49] Initially, the molars were preserved and flushed with PBS containing 2% penicillin-streptomycin. Under the protection of a double layer of sterile gauze, the pulp chamber was crushed with a hammer to expose the pulp tissue.…”

Section: Methodsmentioning

confidence: 99%

A Drop‐By‐Drop Self‐Assembled All‐Natural Hydrogel as a Desensitizer for Rapid and Enduring Management of Dentin Hypersensitivity

Gao,

Liu,

et al. 2024

Adv Healthcare Materials

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Dentin hypersensitivity (DH) is a prevalent dental condition arising from the exposure of dentin tubules (DTs), leading to discomfort upon external stimuli. However, achieving swift and profound occlusion of these exposed DTs for immediate and enduring relief remains challenging due to the intricate dentin structure and oral environment. Herein, we propose a pioneering and facile drop‐by‐drop strategy involving an in‐situ generated natural supramolecular hydrogel formed by self‐assembling silk fibroin (SF) and tannic acid (TA) within the narrow DT space. When SF and TA aqueous solutions are applied successively to exposed dentin, they penetrate deeply within DTs and co‐assemble into compact gels, robustly adhering to DT walls. This yields a rapid and compact occlusion effect with an unprecedented depth exceeding 250 μm, maintaining stable occlusion efficacy even under rigorous in vitro and in vivo erosion and friction conditions for no less than 21 d. Furthermore, the biocompatibility and effective occlusion properties are verified through cell studies in simulated oral settings and an in vivo rabbit model. This study, for the first time, demonstrates the translational potential of hydrogel‐based desensitizers in treating DH with prompt action, superior occlusion depth and enduring treatment benefits, holding promise as clinical‐friendly restorative solutions for delicate‐structured biosystems.This article is protected by copyright. All rights reserved

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“…For example, inspired by biomineralization mechanisms, Yi and coworkers developed a type of multifunctional yolk-shell nanocomposite for treating dentin hypersensitivity. [2] The development of bone substitutes with mineralized collagen matrices has clear and significant clinical needs for hard tissue engineering such as guided bone regeneration (GBR) that has been described in many reports. [30,31] Besides the fields of hard tissue repair and regeneration, collagen mineralization was also important in some other important biomedical engineering fields such as cancer research.…”

Section: Clinical Needs Of In Vitro Biomimetic Mineralization Of Coll...mentioning

confidence: 99%

“…Hard tissues of vertebrates, including bone and dentin, bear dynamic forces from inner and outer environments every day. Knowing clearly the substances and structures of the hard tissues can help us understand the formation mechanism, the origin of some diseases such as caries and osteogenesis imperfecta, [1] and DOI: 10.1002/marc.202200827 find some effective treatments for these diseases (such as dentin desensitization for dentin hypersensitivity treatment [2] ).…”

Section: Introductionmentioning

confidence: 99%

Poly(acrylic acid)‐Assisted Intrafibrillar Mineralization of Type I Collagen: A Review

Chen

Zeng

2023

Macromol. Rapid Commun.

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The mineralization of type I collagen is a biological process occurring in vertebrates by which some hard tissues such as bone and dentin are constructed. Due to the extensive clinical needs for bone defect repair and remineralization of mineral-depleted dentin, biomimetic mineralization of collagen is attracting more and more interests. Synthetic analogs of noncollagenous proteins are necessary for directing the in vitro mineralization. In this paper, the function and mechanism of poly(acrylic acid) (PAA) in regulating the mineralization, especially intrafibrillar mineralization (IM) of collagen are reviewed. As two mineralization patterns (extrafibrillar and intrafibrillar) co-exist in natural hard tissues, differences between them in terms of microstructure, biodegradation, cytocompatibility, osteoinduction in vitro, and performance in vivo are systematically compared. Then the roles of PAA in biomimetic collagen IM within one-analog and two-analog systems are discussed, respectively. Moreover, mineralization of some self-mineralizable collagen matrices is described. Due to the interactions between collagen and PAA play a crucial role in the processes of collagen mineralization, some reference researches are also provided involving the collagen/PAA interactions in some other fields. Finally, this review is ended with an outlook for future potential improvements based on the collection of existing bottlenecks in this field.

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Biomineralization-inspired sandwich dentin desensitization strategy based on multifunctional nanocomposite with yolk–shell structure

Abstract: Dentin hypersensitivity (DH) treatment is far from being unequivocal in providing a superior strategy, which combines immediate and long-term efficiency of dentinal tubules (DTs) occlusion and clinical applicability. In order...

Cited by 8 publications

References 75 publications

Janus Membrane with Intrafibrillarly Strontium-Apatite-Mineralized Collagen for Guided Bone Regeneration

Janus Membrane with Intrafibrillarly Strontium-Apatite-Mineralized Collagen for Guided Bone Regeneration

A Drop‐By‐Drop Self‐Assembled All‐Natural Hydrogel as a Desensitizer for Rapid and Enduring Management of Dentin Hypersensitivity

Poly(acrylic acid)‐Assisted Intrafibrillar Mineralization of Type I Collagen: A Review

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