Biomimmetic surfaces effectively inhibiting coagulation process dedicated for cardiovascular devices

Abstract

Biomimmetic surfaces effectively inhibiting coagulation process dedicated for cardiovascular devicesThe work focused on developing coatings with niche-like surfaces that resemble stem cells. Structures similar to stem cell niches were designed to inhibit the fast division process and subsequently to influence the efficient endothelialisation. Using a plasma method, the surface topography and stiffness, as well as the microstructure, were modified by controlled residual stresses. The cells exhibited a potential for formation of the appropriate endothelial layer. Residual stresses affected the formation of the surface topography in a form of nano-wrinkles similar to niches in the tissue environment where stem cells are stored. Carbon-based films were deposited on reference substrates using a magnetron sputtering technique. Amorphous carbon and diamond like carbon with anode laser source were studied in the work. Niche-like structures were designed to catch stem cells from blood flow and prevent their rapid differentiation. The blood-material interaction under dynamic conditions was performed and described. Blood from above the analysed surface was collected after the test, and the quality of the blood was assessed along with the type of cellular response to the surface. The potential for endothelialisation was adjusted using human umbilical vein endothelium cells. The contact angle measurement was performed giving initial output information for tests on blood. The biomimetic approach considered nichelike structures reconstruction using materials science. In the work we have developed coatings that could mobilize and influence the differentiation of vascular endothelial cells.