Biomimetic tendon extracellular matrix composite gradient scaffold enhances ligament-to-bone junction reconstruction

Liu, Huanhuan; Yang, Long; Zhang, Erchen; Zhang, Rui; Cai, Dandan; Zhu, Shouan; Ran, Jisheng; Bunpetch, Varitsara; Cai, Youzhi; Heng, Boon Chin; Hu, Yin; Dai, Xuesong; Chen, Xiao; Ouyang, Hongwei

doi:10.1016/j.actbio.2017.05.027

Cited by 65 publications

(46 citation statements)

References 42 publications

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“…Some tissues in the body present pronounced gradients in ECM composition and/or cell population, particularly those at interfaces such as the osteochondral interface, the ligament‐to‐bone interface, and the tendon‐to‐bone insertion site . Conventional methods rely on the use of anisotropic scaffolds containing distinctive or continual sections with properties matching those in the native tissues to be engineered .…”

Section: Emerging Evolutions In Bioprintingmentioning

confidence: 99%

3D Bioprinting: from Benches to Translational Applications

Heinrich

Liu

Jimenez

et al. 2019

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Over the last decades, the fabrication of 3D tissues has become commonplace in tissue engineering and regenerative medicine. However, conventional 3D biofabrication techniques such as scaffolding, microengineering, and fiber and cell sheet engineering are limited in their capacity to fabricate complex tissue constructs with the required precision and controllability that is needed to replicate biologically relevant tissues. To this end, 3D bioprinting offers great versatility to fabricate biomimetic, volumetric tissues that are structurally and functionally relevant. It enables precise control of the composition, spatial distribution, and architecture of resulting constructs facilitating the recapitulation of the delicate shapes and structures of targeted organs and tissues. This Review systematically covers the history of bioprinting and the most recent advances in instrumentation and methods. It then focuses on the requirements for bioinks and cells to achieve optimal fabrication of biomimetic constructs. Next, emerging evolutions and future directions of bioprinting are discussed, such as freeform, high-resolution, multimaterial, and 4D bioprinting. Finally, the translational potential of bioprinting and bioprinted tissues of various categories are presented and the Review is concluded by exemplifying commercially available bioprinting platforms. BioprintingThe ORCID identification number(s) for the author(s) of this article can be found under https://doi.

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Section: Emerging Evolutions In Bioprintingmentioning

confidence: 99%

3D Bioprinting: from Benches to Translational Applications

Heinrich

Liu

Jimenez

et al. 2019

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“…Liu et al used random‐aligned‐random decellularized tendon for tendon‐to‐bone junction repair and achieved better results than when using traditional decellularized tendon. However, the boundary between the tendon and bone was still observed after 16 weeks . Moreover, to date, few methods are available for thoroughly removing cells from the enthesis scaffold or decellularization of the whole composite .…”

Section: Introductionmentioning

confidence: 99%

Preparation of Decellularized Triphasic Hierarchical Bone‐Fibrocartilage‐Tendon Composite Extracellular Matrix for Enthesis Regeneration

Zhang

Zhu

et al. 2019

Adv Healthcare Materials

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Tendon to bone (enthesis) rupture, which may cause disability and persistent pain, shows high rate of re‐rupture after surgical repair. Tendon or enthesis scaffolds have been widely studied, but few of these materials can recapitulate the tissue continuity. Thus, this study is conducted to prepare a triphasic decellularized bone‐fibrocartilage‐tendon (D‐BFT) composite scaffold. The D‐BFT scaffold is developed using a combination of physical, chemical, and enzymatic treatments using liquid nitrogen, Triton‐X 100, sodium‐dodecyl sulfate, and DNase I, which effectively removes the cell components while preserving the biological composite and microstructure. Moreover, the mechanical properties of D‐BFT are highly preserved and similar to those of the human Achilles tendon. Additionally, in vitro, mesenchymal stem cells (MSCs) adhered, proliferated, and infiltrated into the D‐BFT scaffold, and MSC differentiation is confirmed by up‐regulation of osteogenic‐related and tenogenic‐related genes. The repair outcomes are explored by applying the D‐BFT scaffold in the model of femur‐tibia defects in vivo, which shows good repair results. Thus, the D‐BFT scaffold developed in this study is a promising graft for enthesis regeneration.

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“…In 2016, Park et al 21 developed a hybrid scaffold with dual configuration of aligned and random electrospun fibers for skeletal muscle tissue engineering and found that the scaffold could still influence the alignment and differentiation of the C2C12 myoblasts cells by controlling the density of aligned layer. Recently, Liu et al 22 physically modified the tendon extracellular matrix into a random-aligned random-composite scaffold using ultrasound treatment and demonstrated that the scaffold could promote osteoinductivity in vitro and enhanced bone and fibrocartilage formation at the interface in vivo. These studies showed the possibility of modifying the scaffold for tendon-to-bone healing by integration of topographic cues.…”

Section: Introductionmentioning

confidence: 99%

Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model

Cai¹,

Wang²,

Ye³

et al. 2018

IJN

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BackgroundTendon/ligament injuries are common sports injuries. Clinically, the repair of a ruptured tendon or ligament to its bony insertion is needed, but the enthesis structure is not well reestablished following surgical repair. Herein, we fabricated dual-layer aligned-random scaffold (ARS) by electrospinning and aimed to investigate the effect of the scaffold on tendon-to-bone healing in vivo.Materials and methodsThe random and dual-layer aligned-random silk fbroin poly(L-lactic acid-co-e-caprolactone) (P(LLA-CL)) nanofibrous scaffolds were successfully fabricated by electrospinning methods. Ninety New Zealand white rabbits were randomly divided into three groups (random scaffold [RS], ARS, and control groups), and they were subjected to surgery to establish an extra-articular tendon-to-bone healing model with autologous Achilles tendon.ResultsHistological assessment showed that the ARS significantly increased the area of metachromasia, decreased the interface width, and improved collagen maturation and organization at the tendon–bone interface compared with the RS and control groups. Microcomputed tomography analysis showed that the bone tunnel area of RS and ARS groups was significantly smaller than those of the control group. Real-time polymerase chain reaction showed that BMP-2 and osteopontin expression levels of the tissue at the interface between the bone and graft in the RS and ARS groups were higher than those of the control group at 6 weeks. Collagen I expression level of the ARS group was significantly higher than those of the RS and control groups at 6 and 12 weeks. Moreover, the ARS groups had a better ultimate load-to-failure and stiffness than the RS and control groups.ConclusionARS could effectively augment the tendon-to-bone integration and improve gradient microstructure in a rabbit extra-articular model by inducing the new bone formation, increasing the area of fibrocartilage, and improving collagen organization and maturation. The dual-layer aligned-random silk fibroin/P(LLA-CL) nanofibrous scaffold is proved to be a promising biomaterial for tendon-to-bone healing.

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Biomimetic tendon extracellular matrix composite gradient scaffold enhances ligament-to-bone junction reconstruction

Cited by 65 publications

References 42 publications

3D Bioprinting: from Benches to Translational Applications

3D Bioprinting: from Benches to Translational Applications

Preparation of Decellularized Triphasic Hierarchical Bone‐Fibrocartilage‐Tendon Composite Extracellular Matrix for Enthesis Regeneration

Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model

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