Biomimetic synthesis of bone-like nanocomposites using the self-organization mechanism of hydroxyapatite and collagen

Kikuchi, Masanori; Ikoma, Toshiyuki; Itoh, Soichiro; Matsumoto, Hiroko; Koyama, Yoshihisa; Takakuda, Kazuo; Shinomiya, Kenichi; Tanaka, Junzo

doi:10.1016/j.compscitech.2003.09.002

Cited by 264 publications

(178 citation statements)

References 19 publications

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“…Calcium phosphate (CaP)/COL composites are a biodegradable artificial bone developed to engineer the organized bone structure, mimicking the biological conditions [5]. These composites are considered one of the most promising biomaterials to replace autologous bones due to structural and biological similarity to this tissue and excellent biocompatibility [1,3,5]. The composite development is one alternative that is being considered and studied to combine the typical bioactive behavior and mechanical properties such as elastic modulus and toughness of some materials and to produce composites with properties closer to bone tissue [3,5].…”

Section: Resultsmentioning

confidence: 99%

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Cytocompatibility evaluation of hydroxyapatite/collagen composites doped with Zn+2

et al. 2007

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The cytocompatibility of synthetic hydroxyapatite/collagen composites alone or doped with Zn +2 was tested by using primary culture of osteoblasts. The hydroxyapatite (HAP) was synthesized having calcium hydroxide and orthophosphoric acid as precursors. A new HAP composite was developed adding 1.05 w% of Zn(NO 3 ) 2 .6H 2 O forming HAPZn. The pure type I collagen (COL) was obtained from bovine pericardium by enzymatic digestion method. The HAP/COL and HAPZn/COL composites were developed and characterized by SEM/EDS. The cell viability and alkaline phosphatase activity in the presence of composites were evaluated by MTT assay and NBT-BCIP assay, respectively, and compared to osteoblastic cells of the control. Three individual experiments were accomplished in triplicates and submitted to the variance analysis and Bonferroni's post-test with statistically significant at p<0.05. The HAPZn/COL composite did not stimulate the proliferation and increasing of alkaline phosphatase activity of the osteoblastic cells. The tested composites did not alter the cellular viability neither caused alterations in the cellular morphology in 72 h showing adequate properties for biological applications. Keywords

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Section: Resultsmentioning

confidence: 99%

“…Calcium phosphates (CaP)/collagen composites have been developed due to its similar composition with the bone tissue [2][3][4]. The main advantageous in collagen load with hydroxyapatite is the modulation of the adhesion process to osteoprogenitor cells migrate and differentiate in substratum [5].…”

Section: Introductionmentioning

confidence: 99%

Cytocompatibility evaluation of hydroxyapatite/collagen composites doped with Zn+2

et al. 2007

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“…Hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 ), as a well known phosphates in the biologically active phosphate ceramic family, has been widely used in the biomedical field, especially in dental and orthopedic surgery (Kapoor and Batra 2010;Kikuchi et al 2004;Liu and Chou 1994;Petit 1999) by virtue of its similarity to natural bone mineral. It is known to be a bioactive and biocompatible material with excellent osteoconductive properties (de Bruijn and van Blitterswijk 1998;Hamadouche and Sedel 2000;Layman and Ardoin 1998), as HA shows a strong interaction with bio-molecules such as collagen (COL), glycoprotein, etc.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and character investigation of new collagen Hydrolysate/polyvinyl alcohol/hydroxyapatite Polymer-Nano-Porous Membranes: I. Experimental design optimization in thermal and structural properties

Imanieh¹,

Aghahosseini²

2013

Syst Synth Biol

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Development of bioorganic-inorganic composites has drawn eyes to extensive attention in biomedical fields and tissue engineering. So many attempts to prepare hydroxyapatite (HA), in conjunction with various binders including polyvinyl alcohol (PVA), and collagen has performed for late 20 years. We applied a method based on the phase separation for making of polymer porous membranes. This procedure is induced through the addition of a small quantity of water (polymer-rich phase) to a solution with HA precursors (polymer-poor phase). Thermal and structural composite properties of collagen Hydrolysate (CH)-PVA/HA Polymer-Nano-Porous Membranes were analyzed by Design of experiment that was undertaken using D-optimal approach, to select the optimal combination of nano composites precursor. The resulted composite characters were investigated by Fourier transform infrared, scanning electron microscopy (SEM) and thermal gravimetric analysis. Based on the SEM images, this new method could be clearly concluded to porous CH-PVA/HA hybrid materials. Finally the hemocompatibility of nanocomposite membranes were evaluated by the hemolysis study.

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“…Kikuchi et al [35] fabricated an artificial bone material having a bone-like nanostructure and chemical composition.…”

Section: The Bone Healing Process and Nanotechnologymentioning

confidence: 99%

Nanotechnology applications in osteodistraction

Singleton¹,

Halen²

2014

Plast Aesthetic Res

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Most current strategies for bone regeneration have relatively satisfactory results. However, there are drawbacks and limitations associated with their use and availability, and even controversial reports about their efficacy and cost-effectiveness. The induction of new bone formation through distraction osteogenesis (DO) is widespread clinical application in the treatment of bone defects, limb deformities, and fracture nonunions. However, a lengthy period of external fixation is usually needed to allow the new bone to consolidate, and complications such as refracture at the distraction gap often occur. Although various biomaterials have been used as injectable delivery systems in DO models, little has been reported on the use of nanobiomaterials as carrier materials for the sustained release of growth factors in bone regeneration. One area of focus in nanotechnology is the delivery of osteogenic factors in an attempt to modulate the formation of bone. This review article seeks to demonstrate the potential of nanobiomaterials to improve biological applications pertinent to osteodistraction.

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Biomimetic synthesis of bone-like nanocomposites using the self-organization mechanism of hydroxyapatite and collagen

Cited by 264 publications

References 19 publications

Cytocompatibility evaluation of hydroxyapatite/collagen composites doped with Zn+2

Cytocompatibility evaluation of hydroxyapatite/collagen composites doped with Zn+2

Synthesis and character investigation of new collagen Hydrolysate/polyvinyl alcohol/hydroxyapatite Polymer-Nano-Porous Membranes: I. Experimental design optimization in thermal and structural properties

Nanotechnology applications in osteodistraction

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