Biomimetic strategy towards gelatin coatings on PET. Effect of protocol on coating stability and cell-interactive properties

Giol, Elena Diana; Vlierberghe, Sandra Van; Unger, Ronald E.; Kersemans, Ken; Vos, Filip De; Kirkpatrick, Charles James; Dubruel, Peter

doi:10.1039/c8tb02676a

Cited by 10 publications

(11 citation statements)

References 41 publications

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“…When compared with PET, the PET-PDA-S carrier could slightly promote cell adhesion and proliferation (Figure S4), which may be due to the adsorption of bioactive factors in the media by PDA coating, thus promoting cell attachment . Compared with PET-PDA-S, the cell density on PET-Gel-S was remarkably higher because gelatin effectively filled the gaps between PET fibers and had excellent cytocompatibility . After culturing for 14 days, the cells on PET-PDA-S increased significantly.…”

Section: Resultsmentioning

confidence: 99%

Autoclavable Polydopamine-Gelatin-Modified Polyethylene Terephthalate Microfibrous Carriers Regulate the Proliferation and Paracrine Signaling of Mesenchymal Stem Cells

et al. 2022

ACS Appl. Polym. Mater.

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Mesenchymal stem cells (MSCs) have attracted considerable attention in cell therapies and tissue engineering for their multilineage differentiation potential and immunomodulatory properties. However, MSCs gradually lose their stemness and multipotentiality in prolonged two-dimensional (2D) culture in vitro. Herein, a three-dimensional (3D) polyethylene terephthalate (PET) microfiber sponge served as the substrate, and gelatin macromolecules were grafted onto PET through the adsorption of polydopamine to prepare a 3D cell carrier (PET-Gel) with an interpenetrating network structure. The cell carrier could be sterilized by autoclaving. The material characterization results showed that the 3D carrier had good coating stability, thermal stability, and mechanical properties; these properties did not decrease after autoclaving. In vitro cell experiments revealed that the 3D cell carrier promoted the adhesion and proliferation of human umbilical cord MSCs (HUCMSCs). In long-term culture (2 weeks), the cell proliferation rate was 2.4-fold higher than that on 2D plates because the interpenetrating network structure of the carrier led to a larger growing area than 2D plates and the cells infiltrated into the interior of the carrier. Simultaneously, HUCMSCs on PET-Gel expressed surface-specific antigens of the MSCs. The typical paracrine cytokine expression was characterized using qPCR and Western blotting. The paracrine function of cells on the 3D carrier was nearly equivalent to that on the 2D plates. These results showed that the PET-Gel carrier was suitable for the long-term culture of HUCMSCs in vitro.

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Section: Resultsmentioning

confidence: 99%

Autoclavable Polydopamine-Gelatin-Modified Polyethylene Terephthalate Microfibrous Carriers Regulate the Proliferation and Paracrine Signaling of Mesenchymal Stem Cells

et al. 2022

ACS Appl. Polym. Mater.

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“…Subsequent to REDV grafting, the stretching frequency of the amide bond belonging to the peptide moiety occurred at 1545.33 cm –1 , representing N–H bending vibration and C–N stretching vibration of the amide bond in REDV . Along with the charge change trend (Figure S1), this result indicated the success of ADA cross-linking and REDV grafting. , …”

Section: Resultsmentioning

confidence: 76%

“…24 Along with the charge change trend (Figure S1), this result indicated the success of ADA cross-linking and REDV grafting. 25,26 As Figure 4 shows, synchronous ADA and EDC/NHS modification effectively improved the stability of AM against enzymatic degradation, and it would result in new tissue regeneration. 2,27,28 When treated with collagenase, fresh AM degraded more exhaustively than the rest of the groups.…”

Section: Resultsmentioning

confidence: 99%

“…HUVECs and HASMCs were separately seeded on samples at a density of 5 × 10 4 cell/mL; after 4 days of incubation, they were observed using SEM. In detail, after 2.5% GA/ PBS fixation for 2 h at 4 °C, various cell-loaded AMs were dehydrated in gradient ethanol (25,50,70,80,90,95,98, and 100%) at each concentration for 15 min and critical-point-dried to maintain the cell morphology. Finally, all gold-coated AMs were analyzed by SEM observation.…”

Section: Materials and Experimental Sectionmentioning

confidence: 99%

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Bioinspired, Artificial, Small-Diameter Vascular Grafts with Selective and Rapid Endothelialization Based on an Amniotic Membrane-Derived Hydrogel

Wang

Cheng

et al. 2020

ACS Biomater. Sci. Eng.

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Clinical application of the amniotic membrane (AM) in vascular reconstruction was limited by poor processability, rapid biodegradation, and insufficient hemocompatibility. In this work, decellularized AM was digested to a thermosensitive hydrogel and densely cross-linked in the nanoscale as “enhanced” collagenous fibers. Via N-(3-dimehylaminopropyl)-N′-ethylcarbodiimide and N-hydroxysuccinimide (EDC/NHS) catalysis, REDV was further grafted to simulate anticoagulant substances on naturally derived blood vessels. This modification approach endowed AM with rapid endothelialization and rare vascular restenosis. Through adjusting the fixation condition, the pore size and mechanical stability of the fiber network were approximate to those of natural tissues and precisely designed to fit for cell adhesion. AM was synchronously fixed by alginate dialdehyde (ADA) and EDC/NHS, forming a “double-cross-linked” stable structure with significantly improved mechanical strength and resistance against enzymic degradation. The hemolytic and platelet adhesion test indicated that ADA/REDV-AM could inhibit hemolysis and coagulation. It also exhibited excellent cytocompatibility. It selectively accelerated adsorption and migration of endothelial cells (ECs) while impeding adhesion and proliferation of smooth muscle cells (SMCs). It maintained EC superiority in competitive growth and avoided thrombosis in vivo. Furthermore, its property of promoting reconstruction and repair of blood vessels was proved in an animal experiment. Overall, the present study demonstrates that ADA/REDV-AM has potential application as a small-diameter artificial vascular intima with rapid endothelialization and reduced SMC/platelet adhesion.

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“…[ 16 ] PEG is considered a biologically inert material with minimal immunogenic properties for safe application in the body, which can improve the pharmacokinetic and pharmacodynamic results of therapeutics by modifying the therapeutic molecules. [ 17–19 ] Moreover, PEG can dissolve in tissue fluid and can be easily excreted from the human body without toxic side effects and prolong the existence time of drugs in the blood circulation. [ 20,21 ] Therefore, PEG is an ideal outer shell material of micelle for a long‐circulating drug carrier.…”

Section: Introductionmentioning

confidence: 99%

Self‐Assembled Star‐Shaped sPCL–PEG Copolymer Nanomicelles with pH‐Sensitivity for Anticancer Drug Delivery

Cheng

Wang

et al. 2020

Macro Chemistry & Physics

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As matters stand, chemotherapy is faced with high toxic and side effects, high risks, and long treatment cycles. Thus, stability, high encapsulation efficiency, intelligent controllability, and good biocompatibility are of great importance for drug delivery vehicles. Herein, a novel doxorubicin hydrochloride (DOX)‐loaded micelle‐like nanoparticle with pH responsive star‐shaped polycaprolactone–polyethyleneglycol copolymer (sPCL–PEG–CT, sPP) is developed through esterification with inositol–polycaprolactone (sPCL) and carboxylated polyethylene glycol (TC–PEG–CT). sPP nanomicelles (sPP NMs) have a high encapsulation efficiency of greater than 85%, an average size of 95 nm, and a zeta potential of −18 mV. In addition, in vitro release tests show that ≈70% of the drug is released from sPP/DOX NMs into phosphate buffer saline solution (PBS) (pH 5.0) at 37 °C for 12 h. More importantly, the cumulative release is ≈90% for 170 h, displaying a more sustainable drug release behavior. The work explores a new synthesis route of star‐shaped polycaprolactone–polyethyleneglycol copolymer NMs. The sPP NMs have a promising future in drug carrier, especially for hydrophobic drugs.

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Biomimetic strategy towards gelatin coatings on PET. Effect of protocol on coating stability and cell-interactive properties

Abstract: The potential in vascular grafts of gelatin-modified poly(ethylene terephthalate) (PET) was shown herein via their coating stability, ability to promote endothelial cells (ECs) and smooth muscle cells (SMCs) and positive cyto- and endotoxicity assessments.

Cited by 10 publications

References 41 publications

Autoclavable Polydopamine-Gelatin-Modified Polyethylene Terephthalate Microfibrous Carriers Regulate the Proliferation and Paracrine Signaling of Mesenchymal Stem Cells

Autoclavable Polydopamine-Gelatin-Modified Polyethylene Terephthalate Microfibrous Carriers Regulate the Proliferation and Paracrine Signaling of Mesenchymal Stem Cells

Bioinspired, Artificial, Small-Diameter Vascular Grafts with Selective and Rapid Endothelialization Based on an Amniotic Membrane-Derived Hydrogel

Self‐Assembled Star‐Shaped sPCL–PEG Copolymer Nanomicelles with pH‐Sensitivity for Anticancer Drug Delivery

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