Biomimetic sensing of Escherichia coli at the solid-liquid interface: From surface-imprinted polymer synthesis toward real sample sensing in food safety

Arreguin-Campos, Rocio; Eersels, Kasper; Lowdon, Joseph W.; Rogosic, Renato; Heidt, Benjamin; Caldara, Manlio; Jiménez-Monroy, Kathia L.; Diliën, Hanne; Cleij, Thomas J.; Grinsven, Bart van

doi:10.1016/j.microc.2021.106554

Cited by 31 publications

(21 citation statements)

References 44 publications

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“…The LoD obtained for neat PDMS SIP was 670 ± 140 CFU/mL, which is in line with previously found values for bacteria-imprinted polyurethane layers. 36 Furthermore, the limit for PDMS-GO SIP is 80 ± 10 CFU/mL, which confirms that the enhancement of the sensor’s signal due to the presence of graphene oxide as additive, leads to an improvement in the overall sensitivity of the device.…”

Section: Resultsmentioning

confidence: 60%

Imprinted Polydimethylsiloxane-Graphene Oxide Composite Receptor for the Biomimetic Thermal Sensing of Escherichia coli

et al. 2022

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This work presents an imprinted polymer-based thermal biomimetic sensor for the detection of Escherichia coli . A novel and facile bacteria imprinting protocol for polydimethylsiloxane (PDMS) films was investigated, and these receptor layers were functionalized with graphene oxide (GO) in order to improve the overall sensitivity of the sensor. Upon the recognition and binding of the target to the densely imprinted polymers, a concentration-dependent measurable change in temperature was observed. The limit of detection attained for the sensor employing PDMS-GO imprints was 80 ± 10 CFU/mL, a full order lower than neat PDMS imprints (670 ± 140 CFU/mL), illustrating the beneficial effect of the dopant on the thermo-dynamical properties of the interfacial layer. A parallel benchmarking of the thermal sensor with a commercial impedance analyzer was performed in order to prove the possibility of using the developed PDMS-GO receptors with multiple readout platforms. Moreover, S. aureus , C. sakazakii and an additional E. coli strain were employed as analogue species for the assessment of the selectivity of the device. Finally, because of the potential that this biomimetic platform possesses as a low-cost, rapid, and on-site tool for monitoring E. coli contamination in food safety applications, spiked fruit juice was analyzed as a real sample. Reproducible and sensitive results fulfill the limit requirements of the applicable European microbiological regulation.

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Section: Resultsmentioning

confidence: 60%

Imprinted Polydimethylsiloxane-Graphene Oxide Composite Receptor for the Biomimetic Thermal Sensing of Escherichia coli

et al. 2022

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“…It is also possible to extend t ward heating-cooling systems by micro-milling similar channels into metal covering the channels with the same technique, thus drastically increasing t between the liquid and the metal substrate. Here, the glass-transition temp be taken into account (75-80 °C for PETG) and serves as an upper limit of t While this can be too low for applications like chemical synthesis, it is still u use-cases such as, for example, biosensing with the heat-transfer method, w ments are often conducted at 37 °C [26][27][28]. Furthermore, in a similar system could be used as electrodes for electrochemical measurements.…”

Section: Application: Roundabout Serpentine Mixermentioning

confidence: 99%

“…Here, the glass-transition temperature has to be taken into account (75-80 • C for PETG) and serves as an upper limit of the application. While this can be too low for applications like chemical synthesis, it is still useful for other use-cases such as, for example, biosensing with the heat-transfer method, where measurements are often conducted at 37 • C [26][27][28]. Furthermore, in a similar system, the substrate could be used as electrodes for electrochemical measurements.…”

Section: Application: Roundabout Serpentine Mixermentioning

confidence: 99%

Topographical Vacuum Sealing of 3D-Printed Multiplanar Microfluidic Structures

et al. 2021

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We demonstrate a novel way of creating three-dimensional microfluidic channels capable of following complex topographies. To this end, substrates with open channels and different geometries were 3D-printed, and the open channels were consecutively closed with a thermoplastic using a low-resolution vacuum-forming approach. This process allows the sealing of channels that are located on the surface of complex multiplanar topographies, as the thermoplastic aligns with the surface-shape (the macrostructure) of the substrate, while the microchannels remain mostly free of thermoplastic as their small channel size resists thermoplastic inflow. This new process was analyzed for its capability to consistently close different substrate geometries, which showed reliable sealing of angles >90°. Furthermore, the thermoplastic intrusion into channels of different widths was quantified, showing a linear effect of channel width and percentage of thermoplastic intrusion; ranging from 43.76% for large channels with 2 mm width to only 5.33% for channels with 500 µm channel width. The challenging sealing of substrate ‘valleys’, which are created when two large protrusions are adjacent to each other, was investigated and the correlation between protrusion distance and height is shown. Lastly, we present three application examples: a serpentine mixer with channels spun around a cuboid, increasing the usable surface area; a cuvette-inspired flow cell for a 2-MXP biosensor based on molecular imprinted polymers, fitting inside a standard UV/Vis-Spectrophotometer; and an adapter system that can be manufactured by one-sided injection molding and is self-sealed before usage. These examples demonstrate how this novel technology can be used to easily adapt microfluidic circuits for application in biosensor platforms.

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“…The HTM is a novel and innovative thermal sensing readout platform developed over the course of last 10 years. 34 The method has received increasing attention in the last few years and has recently been applied in the detection of bacteria and small molecules via the use of surface-imprinted polymers 35 , 36 and MIPs. 37 − 39 In essence, the method is capable of measuring the thermal resistance across a liquid–solid phase boundary, with MIPs being the receptor layer deposited between the two.…”

Section: Introductionmentioning

confidence: 99%

“…The HTM is a novel and innovative thermal sensing readout platform developed over the course of last 10 years . The method has received increasing attention in the last few years and has recently been applied in the detection of bacteria and small molecules via the use of surface-imprinted polymers , and MIPs. − In essence, the method is capable of measuring the thermal resistance across a liquid–solid phase boundary, with MIPs being the receptor layer deposited between the two. As a target analyte is introduced in the liquid phase, it binds to the deposited MIPs, changing its thermodynamic properties, leading to a change in the thermal conduction path between the liquid phase and the solid phase (Figure ).…”

Section: Introductionmentioning

confidence: 99%

Thermal Detection of Glucose in Urine Using a Molecularly Imprinted Polymer as a Recognition Element

et al. 2021

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Glucose bio-sensing technologies have received increasing attention in the last few decades, primarily due to the fundamental role that glucose metabolism plays in diseases (e.g., diabetes). Molecularly imprinted polymers (MIPs) could offer an alternative means of analysis to a field that is traditionally dominated by enzyme-based devices, posing superior chemical stability, cost-effectiveness, and ease of fabrication. Their integration into sensing devices as recognition elements has been extensively studied with different readout methods such as quartz-crystal microbalance or impedance spectroscopy. In this work, a dummy imprinting approach is introduced, describing the synthesis and optimization of a MIP toward the sensing of glucose. Integration of this polymer into a thermally conductive receptor layer was achieved by micro-contact deposition. In essence, the MIP particles are pressed into a polyvinyl chloride adhesive layer using a polydimethylsiloxane stamp. The prepared layer is then evaluated with the so-called heat-transfer method, allowing the determination of the specificity and the sensitivity of the receptor layer. Furthermore, the selectivity was assessed by analyzing the thermal response after infusion with increasing concentrations of different saccharide analogues in phosphate-buffered saline (PBS). The obtained results show a linear range of the sensor of 0.0194–0.3300 mM for the detection of glucose in PBS. Finally, a potential application of the sensor was demonstrated by exposing the receptor layer to increasing concentrations of glucose in human urine samples, demonstrating a linear range of 0.0444–0.3300 mM. The results obtained in this paper highlight the applicability of the sensor both in terms of non-invasive glucose monitoring and for the analysis of food samples.

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Biomimetic sensing of Escherichia coli at the solid-liquid interface: From surface-imprinted polymer synthesis toward real sample sensing in food safety

Cited by 31 publications

References 44 publications

Imprinted Polydimethylsiloxane-Graphene Oxide Composite Receptor for the Biomimetic Thermal Sensing of Escherichia coli

Imprinted Polydimethylsiloxane-Graphene Oxide Composite Receptor for the Biomimetic Thermal Sensing of Escherichia coli

Topographical Vacuum Sealing of 3D-Printed Multiplanar Microfluidic Structures

Thermal Detection of Glucose in Urine Using a Molecularly Imprinted Polymer as a Recognition Element

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