Biomimetic sealant based on gelatin and microbial transglutaminase: An initial <i>in vivo</i> investigation

Liu, Yi; Kopelman, Doron; Wu, Liqun; Hijji, Karam; Attar, Ishay; Preiss-Bloom, Orahn; Payne, Gregory F.

doi:10.1002/jbm.b.31368

Cited by 63 publications

(51 citation statements)

References 106 publications

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“…Gelatin sponge is usually produced by chemical crosslinking and subsequent technical processing, including oriented compression, etc. 4,21,22 In the present study, zein, a hydrophobic protein, was co-electrospun with gelatin to obtain hybrid fibrous membranes. The zein skeleton component maintained structural integrity in contact with PBS without crosslinking.…”

Section: Discussionmentioning

confidence: 99%

Development of gelatin/zein fibrous membranes for hemostatic application

Chen

Zhou

Miao

et al. 2015

Textile Research Journal

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Microporous structures are usually designed for hemostatic agents to dehydrate blood and thus accelerate the natural clotting process. We have developed gelatin-based fibrous membranes by co-electrospinning with a hydrophobic protein, zein, for hemostatic dressings. The hybrid gelatin/zein fibers showed structural integrity upon introduction into an aqueous medium without the subsequent crosslinking of the protein components. Fibrous membranes with different gelatin/zein ratios were characterized by blood absorption, whole blood clotting, and coagulation activity in vitro and hemostatic efficiency in vivo. The results indicated that both blood and simulated body fluid uptake were increased as zein content of hybrid fibers decreased, in correspondence to the increase of hydrophilicity of the membranes. Coagulation activity tests showed that both components did not activate coagulation at all, indicating that the gelatin/ zein fibrous membrane provided a physical matrix for clotting to be initiated. Adequate hemostatic efficacy was observed after applying gelatin/zein fibrous membranes to bleeding sites on the auricular vein, middle auricular artery, and liver of rabbits. In different hemorrhage models, fibrous membranes with a gelatin/zein ratio of 3/1 showed the least bleeding time and blood loss among the other membranes as well as control (gelatin sponge). Moreover, the total blood loss in the liver injury using the membrane (50.7 mg AE 3.8 mg) was significantly less than that of the control (182.5 mg AE 40.3 mg). Overall results demonstrated that gelatin/zein fibrous membranes with fibrous structure and moderate fluid absorption ability showed rapid hemostasis and limited blood loss, which could be of great benefit for hemostatic applications.

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Section: Discussionmentioning

confidence: 99%

Development of gelatin/zein fibrous membranes for hemostatic application

Chen

Zhou

Miao

et al. 2015

Textile Research Journal

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“…Gelatin gels can adhere to both moist and wet tissues with a comparable or superior adhesive strength to fibrin-based sealants [17][18][19]. Liu et al [64] have found that adhesives produced from mTGasecrosslinked gelatin were able to (i) gel in situ within a relevant timeframe (\5 min), (ii) adhere to tissue in the presence of modest amounts of blood, and (iii) possess appropriate mechanical strength to serve as a hemostatic sealant. According to the same approach, Chen et al [18] examined the potential of the gelatin-mTGase adhesive for ophthalmic applications and especially for vitrectomy procedures for retinal reattachment.…”

Section: Transglutaminase In Biomedical Applicationsmentioning

confidence: 98%

Biomimetic Materials for Medical Application Through Enzymatic Modification

Gentile

Chiono

Tonda‐Turo

et al. 2010

Biofunctionalization of Polymers and Their Applications

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Living organisms synthesize functional materials, based on proteins and polysaccharides, using enzyme-catalyzed reactions. According to the biomimetic approach, biomaterial matrices for tissue engineering are designed to be able to mimic the properties and the functions of the extracellular matrix (ECM). In this chapter, the most significant research efforts dedicated to the study and the preparation of biomimetic materials through enzymatic modifications were reviewed. The functionalizations of different polymeric matrices obtained through the catalytic activity of two enzymes (Transglutaminase, TGase and Tyrosinase, TYRase) were discussed. Specifically, the biomimetic applications of TGase and TYRase to confer appropriate biomimetic properties to the biomaterials, such as the possibility to obtain in situ gelling hydrogels and the incorporation of bioactive molecules (growth factors) and cell-binding peptides into the scaffolds, were reviewed.

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“…Gelatin-based sealants have been created using different crosslinking agents, such as genipin [18], water-soluble carbodiimides [18,19], transglutaminase [20,21], biopolymers [22][23][24] and glutaraldehyde [25]. The use of chemical crosslinking agents has a risk of cytotoxicity caused by the unreacted compounds and their degradation products.…”

Section: Surgical Hemostatic Agentsmentioning

confidence: 99%

Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: A dog model study

Yamashita

Tabayashi

et al. 2015

Bio-Medical Materials and Engineering

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BACKGROUND: The bilayer gelatin sealing sheet was developed as a safe, effective, easy-to-handle and low-cost hemostatic agent. OBJECTIVE: To examine the feasibility of gelatin sealing sheets using a canine arterial hemorrhage model. METHODS:In vivo degradation of gelatin sealing sheets was examined by implanting subcutaneously in rats. For the hemostatic and anti-adhesion efficacy investigations, femoral arteries of dogs were pricked with syringe needle to make a small hole and a gelatin (i.e. experimental group) or fibrin glue sealing sheet (i.e. control group) was applied on the hole to stop bleeding (n = 8). After discontinuation of the bleeding, the skin incisions were closed and re-examined 4 weeks postoperatively. RESULTS: From the degradation study, 4 h thermally treated gelatin sheet which degraded within 3 weeks in vivo was chosen for the further hemostatic study. In all cases of gelatin and fibrin glue sealing sheets, bleeding from the needle hole on canine femoral arteries was effectively stopped. Postoperative adhesions and inflammation at the site in the experimental group were significantly less than those in the control group (P < 0.01 for adhesion scores). CONCLUSIONS: The gelatin sealing sheet was found to be as effective as the fibrin glue sealing sheet as a surgical hemostatic agent, and more effective in preventing postoperative adhesions.

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Biomimetic sealant based on gelatin and microbial transglutaminase: An initial in vivo investigation

Cited by 63 publications

References 106 publications

Development of gelatin/zein fibrous membranes for hemostatic application

Development of gelatin/zein fibrous membranes for hemostatic application

Biomimetic Materials for Medical Application Through Enzymatic Modification

Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: A dog model study

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