Biomimetic Replication of Microscopic Metal–Organic Framework Patterns Using Printed Protein Patterns

Abstract

It is demonstrated that metal-organic frameworks (MOFs) can be replicated in a biomimetic fashion from protein patterns. Bendable, fluorescent MOF patterns are formed with micrometer resolution under ambient conditions. Furthermore, this technique is used to grow MOF patterns from fingerprint residue in 30 s with high fidelity. This technique is not only relevant for crime-scene investigation, but also for biomedical applications.

Lanthanide–Organic Frameworks Constructed from an Unsymmetrical Tricarboxylate for Selective Gas Adsorption and Small‐Molecule Sensing

“…[29,[36][37][38][39] The solid-state luminescence properties of Ln-MOF 1 and free H 3 L were investigated at room temperature ( Figure S6). When excited at λ = 378 nm, H 3 L exhibits a broad emission band at λ = 462 nm, which is ascribed to the intraligand π-π* transitions.…”

Synthesis of patterned enzyme–metal–organic framework composites by ink-jet printing

“…The rigid but porous structure of MOFs makes them ideal for immobilizing flexible protein molecules to increase the overall structural stability of protein. Benefited from the protection effect of MOF scaffolds, the incorporated enzyme can resist to various harsh environments that are harmful to fragile native enzyme, (Shieh et al 2015;Liang et al 2015a;Feng et al 2015) even at protein-denaturing conditions (Liao et al 2017;Wu et al 2017). The enhanced stability makes enzyme-MOF composites promising for industrial and biomedical applications such as biocatalysis at harsh conditions (Lykourinou et al 2011;Feng et al 2015;He et al 2016), enzymatic degradation of nerve agents, (Li et al 2016a, b) stabilizing tobacco mosaic virus (Lian et al 2016), and increasing sensitivity of biosensors (Lyu et al 2014;Zhang et al 2017).…”

“…The focus of MOF applications is gas storage, separation, sensing, and catalysis (Furukawa et al 2013;Hayashi et al 2007;Sumida et al 2011;Zhou et al 2012). Moreover, MOFs also have shown great potentials in hosting biomolecules such as enzyme (or protein), explored by pioneering work from several groups in recent years (Lykourinou et al 2011;Chen et al 2012;Deng et al 2012;Lyu et al 2014;Shieh et al 2015;Liang et al 2015a;Feng et al 2015;Li et al 2016a, b, c;Lian et al 2016Lian et al , 2017Liao et al 2017;Zhang et al 2017;Wu et al 2015a, b;Hou and Ge 2017). The rigid but porous structure of MOFs makes them ideal for immobilizing flexible protein molecules to increase the overall structural stability of protein.…”

“…By chemical reactions between amino acids of enzyme and functional groups of pre-synthesized MOFs, enzyme molecules were attached on the surface of MOFs (Jung et al 2011;Shih et al 2012). Alternatively, another strategy is in situ one-pot synthesis of enzyme-MOF composites directly from enzyme, metal ions, and organic ligands in solution, which has been investigated by several groups (Lyu et al 2014;Shieh et al 2015;Liang et al 2015a;Lian et al 2016;Liao et al 2017). All the above methods majorly rely on solutionbased procedures.…”

“…Stimulated by the in situ solution synthesis of enzyme-MOF composites developed previously (Lyu et al 2014;Wu et al 2015a, b;Liang et al 2015a), in this study, for the first time, we use a commercially available color inkjet printer to directly print enzyme-MOF composites on the surface of different substrates including filter paper, polyvinylchloride (PVC) film, poly(ethylene terephthalate) (PET) film, and hydrophilic printing film. Ink solutions containing zinc ions (Zn 2+ ), 2-methylimidazole (2-MeIM), and enzyme molecules were loaded in different cartridges, respectively (C, M, Y cartridges as shown in procedure ① of Fig.…”