Biomimetic Remineralization of Human Enamel in the Presence of Polyamidoamine Dendrimers in vitro

Chen, Liang; He, Yuanqing; Tang, Bei; Liang, Kunneng; Li, Jiyao

doi:10.1159/000375376

Cited by 41 publications

(35 citation statements)

References 29 publications

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“…These amelogenin-inspired dendrimers have been referred to as "artificial proteins" as they can mimic the functions of organic matrices in modulating the biomineralization of tooth enamel. Several in vitro studies have demonstrated that amphiphilic, carboxyl-terminated, and phosphateterminated PAMAM dendrimers exhibited a strong tendency to self-assemble into hierarchical enamel crystal structures [Chen et al, , 2014[Chen et al, , 2015Wu et al, 2013;Yang et al, 2011]. The new crystals created by the PAMAM organic templates had the same structure, orientation, and mineral phase of the intact enamel, with the HA nanorods closely paralleling the original prisms .…”

Section: Amelogeninmentioning

confidence: 99%

State of the Art Enamel Remineralization Systems: The Next Frontier in Caries Management

2018

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The principles of minimally invasive dentistry clearly dictate the need for clinically effective measures to remineralize early enamel caries lesions. While fluoride-mediated remineralization is the cornerstone of current caries management philosophies, a number of new remineralization strategies have been commercialized or are under development that claim to promote deeper remineralization of lesions, reduce the potential risks associated with high-fluoride oral care products, and facilitate caries control over a lifetime. These non-fluoride remineralizing systems can be broadly categorized into biomimetic enamel regenerative technologies and the approaches that repair caries lesions by enhancing fluoride efficacy. This paper discusses the rationale for non-fluoride remineralization and the mechanism of action, challenges, and evidence behind some of the most promising advances in enamel remineralization therapies.

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Section: Amelogeninmentioning

confidence: 99%

State of the Art Enamel Remineralization Systems: The Next Frontier in Caries Management

2018

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“…Up to now the vast majority of the studies with dendritic derivatives addressed the mineralization of CP in bulk aqueous solution for mimicking the effect of noncollagenous proteins, that is, the water‐soluble fraction of proteins. Other studies focused on in vitro remineralization of CP‐based composites such as dentine and enamel . These studies involved various types of dendrimers such as carboxylic acid‐terminated poly(amidoamine) (PAMAM) dendrimers, amine terminated dendrimers such as PAMAM, poly(propylene imine) (PPI), and branched poly(ethylene)imine (PEI), hydroxyl‐terminated PAMAM, and phosphate‐terminated PAMAM .…”

Section: Introductionmentioning

confidence: 99%

“…Other studies focused on in vitro remineralization of CP‐based composites such as dentine and enamel . These studies involved various types of dendrimers such as carboxylic acid‐terminated poly(amidoamine) (PAMAM) dendrimers, amine terminated dendrimers such as PAMAM, poly(propylene imine) (PPI), and branched poly(ethylene)imine (PEI), hydroxyl‐terminated PAMAM, and phosphate‐terminated PAMAM . Several other studies addressed the interaction of these and other polymers with CP crystals, mostly to evaluate the interaction of the terminal functional groups with the CP surface …”

Section: Introductionmentioning

confidence: 99%

A Dendritic Amphiphile for Efficient Control of Biomimetic Calcium Phosphate Mineralization

Hentrich

Taabache

Brezesinski

et al. 2017

Macromolecular Bioscience

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The phase behavior of a dendritic amphiphile containing a Newkome-type dendron as the hydrophilic moiety and a cholesterol unit as the hydrophobic segment is investigated at the air-liquid interface. The amphiphile forms stable monomolecular films at the air-liquid interface on different subphases. Furthermore, the mineralization of calcium phosphate beneath the monolayer at different calcium and phosphate concentrations versus mineralization time shows that at low calcium and phosphate concentrations needles form, whereas flakes and spheres dominate at higher concentrations. Energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, and electron diffraction confirm the formation of calcium phosphate. High-resolution transmission electron microscopy and electron diffraction confirm the predominant formation of octacalcium phosphate and hydroxyapatite. The data also indicate that the final products form via a complex multistep reaction, including an association step, where nano-needles aggregate into larger flake-like objects.

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“…However, due to the lack of an affinity for the enamel surface and self-assembly ability, these ACP nanoparticles cannot efficiently adsorb on the surface of enamel and transform into the oriented and ordered HAP crystals to remineralize enamel. More recently, it has been demonstrated that carboxyl-terminated poly(amido amine) (PAMAM-COOH) can promote the HAP crystallization process and thus has potential for the remineralization of dental hard tissues29. Furthermore, alendronate (ALN) is conjugated on PAMAM-COOH, causing increased binding strength between PAMAM-COOH and the enamel surface due to its specific adsorption on HA (the primary component of tooth enamel)30.…”

mentioning

confidence: 99%

Oriented and Ordered Biomimetic Remineralization of the Surface of Demineralized Dental Enamel Using HAP@ACP Nanoparticles Guided by Glycine

Wang

Xiao

Yang

et al. 2017

Sci Rep

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Achieving oriented and ordered remineralization on the surface of demineralized dental enamel, thereby restoring the satisfactory mechanical properties approaching those of sound enamel, is still a challenge for dentists. To mimic the natural biomineralization approach for enamel remineralization, the biological process of enamel development proteins, such as amelogenin, was simulated in this study. In this work, carboxymethyl chitosan (CMC) conjugated with alendronate (ALN) was applied to stabilize amorphous calcium phosphate (ACP) to form CMC/ACP nanoparticles. Sodium hypochlorite (NaClO) functioned as the protease which decompose amelogenin in vivo to degrade the CMC-ALN matrix and generate HAP@ACP core-shell nanoparticles. Finally, when guided by 10 mM glycine (Gly), HAP@ACP nanoparticles can arrange orderly and subsequently transform from an amorphous phase to well-ordered rod-like apatite crystals to achieve oriented and ordered biomimetic remineralization on acid-etched enamel surfaces. This biomimetic remineralization process is achieved through the oriented attachment (OA) of nanoparticles based on non-classical crystallization theory. These results indicate that finding and developing analogues of natural proteins such as amelogenin involved in the biomineralization by natural macromolecular polymers and imitating the process of biomineralization would be an effective strategy for enamel remineralization. Furthermore, this method represents a promising method for the management of early caries in minimal invasive dentistry (MID).

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Biomimetic Remineralization of Human Enamel in the Presence of Polyamidoamine Dendrimers in vitro

Cited by 41 publications

References 29 publications

State of the Art Enamel Remineralization Systems: The Next Frontier in Caries Management

State of the Art Enamel Remineralization Systems: The Next Frontier in Caries Management

A Dendritic Amphiphile for Efficient Control of Biomimetic Calcium Phosphate Mineralization

Oriented and Ordered Biomimetic Remineralization of the Surface of Demineralized Dental Enamel Using HAP@ACP Nanoparticles Guided by Glycine

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