Biomimetic pH Sensitive Polymersomes for Efficient DNA Encapsulation and Delivery

Lomas, Hannah; Cantón, Irene; MacNeil, Sheila; Du, Jianzhong; Armes, Steven P.; Ryan, Anthony J.; Lewis, Andrew L.; Battaglia, Giuseppe

doi:10.1002/adma.200700941

Cited by 425 publications

(435 citation statements)

References 47 publications

(58 reference statements)

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“…PMPC 25 -PDPA 70 copolymer synthesis The PMPC-b-PDPA diblock copolymer was prepared by ATRP (50). In a typical ATRP procedure, a Schlenk flask with a magnetic stir bar and a rubber septum was charged with MPC (1.32 g, 4.46 mmol) and ME-Br initiator (50.0 mg, 0.178 mmol) in ethanol (4 ml) and purged for 30 min with N 2 .…”

Section: Methodsmentioning

confidence: 99%

Chemotactic synthetic vesicles: design and applications in blood brain barrier crossing

Joseph

Contini

Cecchin

et al. 2016

Preprint

114

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In recent years, scientists have created artificial microscopic and nanoscopic self-propelling particles, often referred to as nano-or microswimmers, capable of mimicking biological locomotion and taxis. This active diffusion enables the engineering of complex operations that so far have not been possible at the micro-and nanoscale. One of the most promising tasks is the ability to engineer nanocarriers that can autonomously navigate within tissues and organs, accessing nearly every site of the human body guided by endogenous chemical gradients. We report a fully synthetic, organic, nanoscopic system that exhibits attractive chemotaxis driven by enzymatic conversion of glucose. We achieve this by encapsulating glucose oxidase alone or in combination with catalase into nanoscopic and biocompatible asymmetric polymer vesicles (known as polymersomes). We show that these vesicles self-propel in response to an external gradient of glucose by inducing a slip velocity on their surface, which makes them move in an extremely sensitive way toward higher-concentration regions. We finally demonstrate that the chemotactic behavior of these nanoswimmers, in combination with LRP-1 (low-density lipoprotein receptor-related protein 1) targeting, enables a fourfold increase in penetration to the brain compared to nonchemotactic systems.

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Section: Methodsmentioning

confidence: 99%

Chemotactic synthetic vesicles: design and applications in blood brain barrier crossing

Joseph

Contini

Cecchin

et al. 2016

Preprint

114

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“…[99] The use of these highly biocompatible PMPC-PDPA copolymer vesicles as non-toxic synthetic vectors for the in vitro delivery of either DNA or dye molecules to living cells has been demonstrated. [100,101] DNA-loaded PMPC-PDPA vesicles undergo rapid endocytosis and the lower local pH within the endocytic organelle (pH 5-6) causes protonation of the hydrophobic PDPA chains, vesicle dissociation, and hence triggered release of the payload. High transfection efficiencies have been demonstrated using Human Dermal Fibroblast (HDF) cell lines for these delivery vehicles.…”

Section: Reactive and Environmentally Responsive Membranes For Delivementioning

confidence: 99%

Self‐Assembled Block Copolymer Aggregates: From Micelles to Vesicles and their Biological Applications

Blanazs

Armes

Ryan

2009

Macromol. Rapid Commun.

1,392

1,370

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The ability of amphiphilic block copolymers to self‐assemble in selective solvents has been widely studied in academia and utilized for various commercial products. The self‐assembled polymer vesicle is at the forefront of this nanotechnological revolution with seemingly endless possible uses, ranging from biomedical to nanometer‐scale enzymatic reactors. This review is focused on the inherent advantages in using polymer vesicles over their small molecule lipid counterparts and the potential applications in biology for both drug delivery and synthetic cellular reactors.magnified image

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“…Moreover, targeted transport can be achieved by taking advantage of the many possibilities to endfunctionalize the copolymers (5). The controlled release of therapeutic substances can also be integrated through the use of copolymers with blocks that respond to chemical stimuli such as hydrolysis (6,7), oxidation (8) or reduction (9) reaction, and pH changes (10)(11)(12)(13)(14)(15)(16)(17). Common strategies have been to use hydrophobic blocks that can progressively degrade or convert into hydrophilic moieties or to use a cleaveable linkage between hydrophobic and hydrophilic blocks.…”

mentioning

confidence: 99%

Bursting of sensitive polymersomes induced by curling

Mabrouk

Cuvelier

Brochard‐Wyart

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

175

152

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Polymersomes, which are stable and robust vesicles made of block copolymer amphiphiles, are good candidates for drug carriers or micro/nanoreactors. Polymer chemistry enables almost unlimited molecular design of responsive polymersomes whose degradation upon environmental changes has been used for the slow release of active species. Here, we propose a strategy to remotely trigger instantaneous polymersome bursting. We have designed asymmetric polymer vesicles, in which only one leaflet is composed of responsive polymers. In particular, this approach has been successfully achieved by using a UV-sensitive liquid-crystalline copolymer. We study experimentally and theoretically this bursting mechanism and show that it results from a spontaneous curvature of the membrane induced by the remote stimulus. The versatility of this mechanism should broaden the range of applications of polymersomes in fields such as drug delivery, cosmetics and material chemistry.asymmetric polymer vesicles ͉ liquid-crystalline copolymer ͉ spontaneous curvature ͉ UV-sensitive

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Biomimetic pH Sensitive Polymersomes for Efficient DNA Encapsulation and Delivery

Cited by 425 publications

References 47 publications

Chemotactic synthetic vesicles: design and applications in blood brain barrier crossing

Chemotactic synthetic vesicles: design and applications in blood brain barrier crossing

Self‐Assembled Block Copolymer Aggregates: From Micelles to Vesicles and their Biological Applications

Bursting of sensitive polymersomes induced by curling

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