Biomimetic mineralization of collagen fibrils induced by amine-terminated PAMAM dendrimers – PAMAM dendrimers for remineralization

Liang, Kunneng; Gao, Yuan; Li, Jianshu; Liao, Ying; Xiao, Shimeng; Zhou, Xuedong

doi:10.1080/09205063.2015.1068606

Cited by 32 publications

(32 citation statements)

References 45 publications

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“…Previous studies showed that some calcium phosphate compounds were only suitable for remineralizing the partially-demineralized dentin, which relied on the epitaxial deposition of calcium and phosphate ions over remnant crystallites [12,17]. In some clinical situations, dentin is completely demineralized and the collagen fibril is exposed.…”

Section: Discussionmentioning

confidence: 99%

“…However, PAMAM was found to have the ability to remineralize not only the partially-demineralized dentin, but also collagen fibrils. This is because PAMAM is a biomimetic analog to NCPs and can induce biomimetic remineralization based on a particle-based crystallization concept [12,22]. PAMAM can perform its function when bonded to a surface and dentin collagen fibers.…”

Section: Discussionmentioning

confidence: 99%

“…The disks were divided into four groups randomly ( n = 6). A 0.5 M EDTA (Ethylene diamine tetraacetic acid disodium salt) solution (pH 7.4) was used to prepare dentin erosion for 5 min as a previous study showed [12]. Then, dentin disks were rinsed with deionized water (DI) water and ultrasonically cleaned for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Then, the disks were immersed into artificial saliva solution for 14 days. The artificial saliva solution was refreshed every 24 h [12]. After 14 days, the dentin disks were air-dried and sputter-coated with gold examining via SEM Quanta 200 (FEI, Hillsboro, OR, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Another method to obtain anti-caries adhesives was to incorporate remineralization agents into adhesive systems, including calcium phosphate compounds and biomimetic mineralization agents [12]. A previous study showed adhesive containing nanoparticles of amorphous calcium phosphate (NACP) could induce the formation of tertiary dentin in a rat cavity model [13].…”

Section: Introductionmentioning

confidence: 99%

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Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

et al. 2017

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The mechanical properties and anti-caries effect of a novel anti-caries adhesive containing poly (amidoamine) dendrimer (PAMAM) and dimethylaminododecyl methacrylate (DMADDM) were investigated for the first time. Microtensile bond strength and surface charge density were measured for the novel anti-caries adhesives. Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii were chosen to form three-species biofilms. Lactic acid assay, MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, exopolysaccharide staining and live/dead staining were performed to study anti-biofilm effect of the adhesive. The TaqMan realtime polymerase chain reaction was used to study the proportion change in three-species biofilms of different groups. The Scanning Electron Microscope (SEM) was used to observe the remineralization effect of PAMAM and DMADDM. The results showed that incorporating PAMAM and DMADDM into adhesive had no adverse effect on the dentin bond strength. The 1% PAMAM and 5% DMADDM adhesive group showed anti-biofilm properties and developed a healthier biofilm with a lower chance of inducing dental caries. Combination of 1% PAMAM and 5% DMADDM solution maintained remineralization capability on dentin, similar to that using 1% PAMAM alone. In conclusion, the adhesive containing PAMAM and DMADDM had strong antimicrobial properties and biological remineralization capabilities, and is promising for anti-caries clinical applications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

et al. 2017

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Complementarity and Uncertainty in Intrafibrillar Mineralization of Collagen

Jiao

Niu

Fan

et al. 2016

Adv Funct Materials

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Biomineralization in vertebrates is a ubiquitous and tightly regulated process which creates hierarchical structures for the skeleton. Because of the lack of understanding and applicability of cell‐based or biological systems to achieve intrafibrillar mineralization, scientists adopted various in vitro methods to elucidate the mechanism of intrafibrillar mineralization. In this article, biomimetic intrafibrillar mineralization of collagen in its wide ramifications is reviewed. It is intriguing how prevailing intrafibrillar mineralization mechanisms derived from two potentially discordant crystallization philosophies were equally adept, depending on the experimental context, at theorizing the formation of calcium phosphate within a fibrillar template. This complementarity is not unique to biomineralization and has precedence in other fundamental physical interpretations. A new intrafibrillar mineralization process based on the use of polycationic process‐directing agent added uncertainty to the use of existing mechanisms in accounting for the observations.

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Biomineralization‐Inspired Material Design for Bone Regeneration

Pereira

Habibović

2018

Adv Healthcare Materials

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Synthetic substitutes of bone grafts, such as calcium phosphate–based ceramics, have shown some good clinical successes in the regeneration of large bone defects and are currently extensively used. In the past decade, the field of biomineralization has delivered important new fundamental knowledge and techniques to better understand this fascinating phenomenon. This knowledge is also applied in the field of biomaterials, with the aim of bringing the composition and structure, and hence the performance, of synthetic bone graft substitutes even closer to those of the extracellular matrix of bone. The purpose of this progress report is to critically review advances in mimicking the extracellular matrix of bone as a strategy for development of new materials for bone regeneration. Lab‐made biomimicking or bioinspired materials are discussed against the background of the natural extracellular matrix, starting from basic organic and inorganic components, and progressing into the building block of bone, the mineralized collagen fibril, and finally larger, 2D and 3D constructs. Moreover, bioactivity studies on state‐of‐the‐art biomimicking materials are discussed. By addressing these different topics, an overview is given of how far the field has advanced toward a true bone‐mimicking material, and some suggestions are offered for bridging current knowledge and technical gaps.

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Biomimetic mineralization of collagen fibrils induced by amine-terminated PAMAM dendrimers – PAMAM dendrimers for remineralization

Abstract: In this study, we successfully induced biomimetic mineralization within type-I collagen fibrils using G3-PAMAM-NH2. This strategy may serve as a potential therapeutic technique for restoring completely demineralized collagenous mineralized tissues.

Cited by 32 publications

References 45 publications

Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

Complementarity and Uncertainty in Intrafibrillar Mineralization of Collagen

Biomineralization‐Inspired Material Design for Bone Regeneration

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