Biomimetic macroporous hydrogel scaffolds in a high‐throughput screening format for cell‐based assays

Dainiak, Maria B.; Savina, Irina N.; Musolino, Isabella; Kumar, Ashok; Mattìasson, Bo; Galaev, Igor Yu.

doi:10.1002/btpr.30

Cited by 31 publications

(45 citation statements)

References 55 publications

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“…Polymerisation or crosslinking of a precursor in these liquid inter-crystallite layers results in the formation of relatively thin nano/microporous walls of macropores [2][3][4][30][31][32][33][34][35][36][37][38][39]. The growth of ice crystallites can cause a significant pressure on the formed pore walls.…”

Section: Cryotropic Gelation Features and The Nature Of Freezing Processmentioning

confidence: 99%

“…This is due to the interconnected system of macropores, and the spongy and elastic morphology of the cryogels as a whole [2][3][4][30][31][32][33][34][35][36][37][38][39][40]. These features, in combination with a molecular-imprinting polymer (MIP) technique, provide a new approach for various biotechnological and environmental applications [2,3].…”

Section: Cryotropic Gelation Features and The Nature Of Freezing Processmentioning

confidence: 99%

“…There are, at least, four different types of materials named "cryogels": (i) gelatinous precipitate forming during cryoprecipitationblood plasma treatment upon cooling to ca. 4°C [57]; (ii) frozen polymeric or other hydrogels used 14 as antipyretic materials or materials for thermal energy storage [58]; (iii) polymeric and inorganic cryogels produced using sol-gel technology with subsequent freeze-drying including individual and mixed inorganic oxides, polymers, hybrids, composites, and carbon cryogels (the latter is prepared by carbonisation of polymeric cryogels) [59][60][61][62][63][64][65][66][67] There are many cryogels prepared from synthetic polymers such as poly(vinyl alcohol), PVA, which was one of the first polymers used in cryogel preparation [2][3][4]36,38,74,78,82]. PVA alone or in combination with other polymers or proteins is very popular material for the cryogel preparation because its structure containing the (-CH 2 -C(OH)H-) n chain allows various degrees of cross-linking with the participation of the active COH groups, different cross-linkers and co-polymers.…”

Section: Types Of Cryogel Based Composite and Hybrid Materialsmentioning

confidence: 99%

“…The comparative structural and other characterisation of PVA and AC/PVA cryogels is described below using a variety of experimental and theoretical approaches. In this paper, which is mainly devoted to description of methods and approaches for morphological, structural and adsorption characterisation of cryogels, synthesis of cryogels is described only briefly, and the reader is referred to other review papers and books where the synthesis of cryogels is given in detail [2][3][4]36,38,74,78].…”

Section: Types Of Cryogel Based Composite and Hybrid Materialsmentioning

confidence: 99%

“…Therefore, determination of the amounts of different types of water in cryogels [50,51,54] is of importance to understand the nature of adsorption/desorption and diffusion processes within the pore network in cryogels, as well as to estimate the biocompatibility and structural organisation of whole cryogels [2][3][4][10][11][12][13][14][15][16][17][18][19][20][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49]54]. A variety of experimental methods sensitive to the water state can be used for these purposes [50][51][52][53][54][55][56].…”

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confidence: 99%

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Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

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281

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*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

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Section: Cryotropic Gelation Features and The Nature Of Freezing Processmentioning

confidence: 99%

Section: Cryotropic Gelation Features and The Nature Of Freezing Processmentioning

confidence: 99%

Section: Types Of Cryogel Based Composite and Hybrid Materialsmentioning

confidence: 99%

Section: Types Of Cryogel Based Composite and Hybrid Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

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Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

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281

205

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Engineering 3D Hydrogels for Personalized In Vitro Human Tissue Models

Liaw

Guvendiren

2018

Adv Healthcare Materials

111

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There is a growing interest in engineering hydrogels for 3D tissue and disease models. The major motivation is to better mimic the physiological microenvironment of the disease and human condition. 3D tissue models derived from patients' own cells can potentially revolutionize the way treatment and diagnostic alternatives are developed. This requires development of tissue mimetic hydrogels with user defined and tunable properties. In this review article, a recent summary of 3D hydrogel platforms for in vitro tissue and disease modeling is given. Hydrogel design considerations and available hydrogel systems are summarized, followed by the types of currently available hydrogel models, such as bulk hydrogels, porous scaffolds, fibrous scaffolds, hydrogel microspheres, hydrogel sandwich systems, microwells, and 3D bioprinted constructs. Although hydrogels are utilized for a wide range of tissue models, this article focuses on liver and cancer models. This article also provides a detailed section on current challenges and future perspectives of hydrogel-based tissue models.

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Latest Advances in Cryogel Technology for Biomedical Applications

Memić

Colombani

Eggermont

et al. 2019

Advanced Therapeutics

229

241

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There exists a technological need for advanced materials with improved properties for emerging biomedical applications. Recent developments in macroporous materials have demonstrated their applicability as indispensable tools in biomedical research. Cryogels, which are materials with a macroporous 3D structure, are produced as a result of controlled freezing during polymerization with a highly interconnected polymer network. Cryogels’ interest lies in their ability to address some of the limitations of their hydrogel analogues. In this review, hydrogel and cryogel basic concepts are discussed as a short primer for readers unfamiliar with the cryogels literature. Next, a general overview of the methods for synthesis and characterization of cryogels is provided, highlighting key concepts relevant to cryogels and explaining their unique properties. Finally an in‐depth overview of specific technologies and fields where cryogels have been applied is given. It is argued that the latest advances in cryogel technologies are able to address challenges in bioseparation, tissue engineering, and other emerging bioengineering disciplines.

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Biomimetic macroporous hydrogel scaffolds in a high‐throughput screening format for cell‐based assays

Cited by 31 publications

References 55 publications

Cryogels: Morphological, structural and adsorption characterisation

Cryogels: Morphological, structural and adsorption characterisation

Engineering 3D Hydrogels for Personalized In Vitro Human Tissue Models

Latest Advances in Cryogel Technology for Biomedical Applications

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