Biomimetic ion nanochannels for sensing umami substances

Li, Mingyang; Zhang, Ninglong; Cui, Zhiyong; Wang, Wenli; Wang, Cunli; Wang, Dongdong; Li, Minmin; Lü, Wenqi; Qing, Guangyan; Liu, Yuan

doi:10.1016/j.biomaterials.2022.121418

Cited by 16 publications

(23 citation statements)

References 53 publications

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“…Here, the α-helix decreased in the presence of each umami ligand, indicating that the binding mode disturbed the HB network of T1R1-VFT to some extent and further resulted in the partial unfolding near the binding region. The findings were consistent with our recent study based on a T1R1-VFT type biomimetic conical nanochannels system . The binding of IMP decreased α-helix proportion of T1R1-VFT, which further changed the spatial configuration of various groups and increase the negative charge density of the receptor …”

Section: Resultssupporting

confidence: 92%

“…The findings were consistent with our recent study based on a T1R1-VFT type biomimetic conical nanochannels system . The binding of IMP decreased α-helix proportion of T1R1-VFT, which further changed the spatial configuration of various groups and increase the negative charge density of the receptor …”

Section: Resultssupporting

confidence: 92%

“…In T1R1-VFT/MSG/ l -Glu complex, residues T149, S172, and Y220 mainly responded to l -Glu/MSG via directly interacting with C α -NH 3 + and C α -COO – of MSG (Table ). ,,, In the T1R1-VFT/WSA complex, one COO – of WSA presented a quite similar binding model to the C α -COO – of MSG (residues R281, S306 as the binding sites), while the other COO – formed HB with S300 and A302 . As for T1R1-VFT/BMP complex, it was the residues N69, N150, R151, Y220, and S276 mainly bound with BMP (Table ).…”

Section: Resultsmentioning

confidence: 98%

“…Specially, a dramatic red shift (275–286 nm) was observed when the increase of IMP concentrations at Δλ = 60 nm, meaning that the interaction between T1R1-VFT and IMP enhanced the polarity around Trp residues and decreased the hydrophobicity. In other words, the fluorophores of T1R1-VFT were placed in a less hydrophobic environment and more exposed to the solution with the addition of IMP. , Hence, the microenvironment around Trp and Tyr residues was changed in the binding process of the umami receptor–ligand, and in turn, it may be devoted to the conformational change of T1R1-VFT …”

Section: Resultsmentioning

confidence: 99%

“…As such, obtaining a sufficient amount of high purity extracellular domains of T1Rs and elucidating their functional activity and structural characteristics were the main current concern. − Simultaneously, computational procedures were performed to delineate the binding model of T1R1-VFT/umami ligands. ,, Using molecular dynamics simulations, Liu et al reported two major adjacent binding sites in T1R1-VFT, and five umami ligands, MSG, disodium succinate (WSA, sodium salt of succinic acid), guanosine-5′-monophosphate (GMP), IMP, and beefy meaty peptide (BMP), were able to bind one or both of those sites depending on their molecular size and chemical properties . In addition, T1R1-VFT based biosensors were developed to delineate the umami receptor–ligand interaction and umami substances sensing, , and it was found that the sensing ability of T1R1-VFT toward four umami ligands were GMP, MSG, IMP, and WSA in descending order …”

Section: Introductionmentioning

confidence: 99%

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Typical Umami Ligand-Induced Binding Interaction and Conformational Change of T1R1-VFT

Zhang

Cui

et al. 2022

J. Agric. Food Chem.

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Umami taste receptor type 1 member 1/3 (T1R1/T1R3) heterodimer has multiple ligand-binding sites, most of which are located in T1R1-Venus flytrap domain (T1R1-VFT). However, the critical binding process of T1R1-VFT/umami ligands remains largely unknown. Herein, T1R1-VFT was prepared with a sufficient amount and functional activity, and its binding characteristics with typical umami molecules (monosodium l-glutamate, disodium succinate, beefy meaty peptide, and inosine-5′-monophosphate) were explored via multispectroscopic techniques and molecular dynamics simulation. The results showed that, driven mainly by hydrogen bond, van der Waals forces, and electrostatic interactions, T1R1-VFT bound to umami compound at 1:1 (stoichiometric interaction) and formed T1R1-VFT/ligand complex (static fluorescence quenching) with a weak binding affinity (K a values: 252 ± 19 to 1169 ± 112 M–1). The binding process was spontaneous and exothermic (ΔG, −17.72 to −14.26 kJ mol–1; ΔH, −23.86 to −12.11 kJ mol–1) and induced conformational changes of T1R1-VFT, which was mainly reflected in slight unfolding of α-helix (Δα-helix < 0) and polypeptide chain backbone structure. Meanwhile, the binding of the four ligands stabilized the active conformation of the T1R1-VFT pocket. This work provides insight into the binding interaction between T1R1-VFT/umami ligands and improves understanding of how umami receptor recognizes specific ligand molecules.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Typical Umami Ligand-Induced Binding Interaction and Conformational Change of T1R1-VFT

Zhang

Cui

et al. 2022

J. Agric. Food Chem.

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Multifunctional Biomimetic Microstructured Surfaces for Healthcare Applications

Jia

Jiang

Zhang

et al. 2022

Adv Materials Inter

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The wings of butterflies and birds prevent wetting by rain and maintain the capability of flight. Water rolls in droplets on the surface of lotus leaves without collapsing to wet the whole surface, leading to the discovery of superhydrophobicity. The superhydrophobicity originates from the synergistic effect of the hierarchical microarray structure of the lotus leaf surface and low surface energy substances. The superhydrophobic surfaces have been applied in the fields of self-cleaning surfaces, [8] liquid transportation, [9] and antifouling surfaces. [10] The

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Recent Advances in the Modification and Characterization of Solid‐State Nanopores

Lü

Cao

Qing

2022

Chemistry An Asian Journal

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Nanopores, due to their advantages of being modifiable, controllability and sensitivity, have made a splash in recent years in the fields of biomolecular sequencing, small molecule detection, salt differential power generation, and biomimetic ion channels. In these applications, the role of chemical or biological modification is indispensable. Compared with small molecules, the modification of polymers is more difficult and the methods are more diverse. Choosing an appropriate modification method directly determines the success or failure of a research project; therefore, it is necessary to summarize polymer modification methods toward nanopores. In addition, it is also important to provide clear and convincing evidence that the nanopore modification is successful, the corresponding characterization methods are also indispensable. Therefore, this review will summarize the methods of polymer modification of nanopores and efficient characterization methods. We hope that this review will provide some reference value for like-minded researchers.

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Biomimetic ion nanochannels for sensing umami substances

Cited by 16 publications

References 53 publications

Typical Umami Ligand-Induced Binding Interaction and Conformational Change of T1R1-VFT

Typical Umami Ligand-Induced Binding Interaction and Conformational Change of T1R1-VFT

Multifunctional Biomimetic Microstructured Surfaces for Healthcare Applications

Recent Advances in the Modification and Characterization of Solid‐State Nanopores

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