Biomimetic immunomagnetic gold hybrid nanoparticles coupled with inductively coupled plasma mass spectrometry for the detection of circulating tumor cells

Chang, Zhimin; Zhou, Hang; Yang, Chao; Zhang, Rui; You, Qiannan; Yan, Rui; Li, Li; Ge, Ming‐Feng; Tang, Yuguo; Dong, Wen‐Fei; Wang, Zheng

doi:10.1039/d0tb00403k

Cited by 22 publications

(18 citation statements)

References 48 publications

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“…It should be emphasized, there are barely no cases reporting the exact numbers of non‐specifically caught WBCs from the non‐pretreated whole blood (samples of patients or model animals) using the immunomagnetic bead separation approach, although remained WBCs background has been universally recognized as interference to downstream tumor‐related analyses. In addition to the mimic samples with spiked tumor cells, two major techniques were employed to reduce WBCs from whole blood samples: 1) depletion of red blood cells (RBCs) through RBCs lysis [ 21 ] or depletion of WBCs with anti‐CD45 modified magnetic beads, [ 48 ] 2) microfluidic separation. [ 49 ] All these techniques may decrease WBCs numbers to several thousands, and even several hundreds.…”

Section: Resultsmentioning

confidence: 99%

“…For example, a leukocyte membrane camouflaged magnetic nanoparticle system decorated with anti‐epithelial cell adhesion molecule (anti‐EpCAM) antibodies was developed to promote targeting toward MCF‐7 cells, 67% of spiked MCF‐7 cells together with 254 leukocytes were separated from lysed human blood. [ 21 ] Similarly, incorporating bovine serum albumin and folic acid onto magnetic beads enabled high specific isolation of CTCs, demonstrating a CTCs capture efficiency of 75.7% and decreased nonspecific capture efficiency from ≈30% to ≈18%. [ 22 ] However, it should be noticed that the current mostly employed surface modifications may hinder wide application of the immunomagnetic beads for separation of objective CTCs during the complicated cancer progression.…”

Section: Introductionmentioning

confidence: 99%

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Cell‐Released Magnetic Vesicles Capturing Metabolic Labeled Rare Circulating Tumor Cells Based on Bioorthogonal Chemistry

Kang

Zhou

Zhang

et al. 2021

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Capture of circulating tumor cells (CTCs) with high efficiency and high purity holds great value for potential clinical applications. Besides the existing problems of contamination from blood cells and plasma proteins, unknown/down‐regulated expression of targeting markers (e.g., antigen, receptor, etc.) of CTCs have questioned the reliability and general applicability of current CTCs capture methodologies based on immune/aptamer‐affinity. Herein, a cell‐engineered strategy is designed to break down such barriers by employing the cell metabolism as the leading force to solve key problems. Generally, through an extracellular vesicle generation way, the cell‐released magnetic vesicles inherited parent cellular membrane characteristics are produced, and then functionalized with dibenzoazacyclooctyne to target and isolate the metabolic labeled rare CTCs. This strategy offers good reliability and broader possibilities to capture different types of tumor cells, as proven by the capture efficiency above 84% and 82% for A549 and HepG2 cell lines as well as an extremely low detection limitation of 5 cells. Moreover, it enabled high purity enrichment of CTCs from 1 mL blood samples of tumor‐bearing mice, only ≈5–757 white blood cells are non‐specific caught, ignoring the potential phenotypic fluctuation associated with the cancer progression.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cell‐Released Magnetic Vesicles Capturing Metabolic Labeled Rare Circulating Tumor Cells Based on Bioorthogonal Chemistry

Kang

Zhou

Zhang

et al. 2021

Small

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“…Gd-based NPs have shown great utility as MRI and CT contrast agents and as radiosensitizers due to their paramagnetic property and high X-ray attenuation coefficient [ 83 ]. Gold NPs are ideal for creating highly selective, versatile, and sensitive biosensors, capable of optical and electrical detection, surface plasmon resonance, and fluorescence resonance energy transfer [ 84 , 85 ]. Nanomaterials can enable early detection of circulating tumor cells (CTCs) from peripheral blood, as was shown using magnetic NPs functionalized with polyethyleneimine/protein corona or in a separate study, tannic acid [ 86 , 87 ].…”

Section: Principles Of Nanotechnologymentioning

confidence: 99%

Cancer nanotechnology: current status and perspectives

2021

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Modern medicine has been waging a war on cancer for nearly a century with no tangible end in sight. Cancer treatments have significantly progressed, but the need to increase specificity and decrease systemic toxicities remains. Early diagnosis holds a key to improving prognostic outlook and patient quality of life, and diagnostic tools are on the cusp of a technological revolution. Nanotechnology has steadily expanded into the reaches of cancer chemotherapy, radiotherapy, diagnostics, and imaging, demonstrating the capacity to augment each and advance patient care. Nanomaterials provide an abundance of versatility, functionality, and applications to engineer specifically targeted cancer medicine, accurate early-detection devices, robust imaging modalities, and enhanced radiotherapy adjuvants. This review provides insights into the current clinical and pre-clinical nanotechnological applications for cancer drug therapy, diagnostics, imaging, and radiation therapy.

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“…Systems for the detection of CTCs have become popular, since their enumeration is relevant for cancer prognosis and their study could improve treatment effectiveness [67,68]. Various techniques including mass spectrometry, electromagnetic spectroscopy, fluorescence, and electrochemistry have been applied for on-chip early detection or capture of cancer cells [39,[69][70][71][72]. Modern fluorescence-activated cell sorting machines can optically process large numbers of cells in reduced time, but requiring sophisticated optical instrumentation and analysis software, which are very costly and not possible to transform in easy-to-use diagnostic tools [73].…”

Section: On-chip Electrochemical Sensing In 3d Cell Culturementioning

confidence: 99%

Electrochemical Sensing in 3D Cell Culture Models: New Tools for Developing Better Cancer Diagnostics and Treatments

Oliveira

Conceição

Kant

et al. 2021

Cancers

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Currently, conventional pre-clinical in vitro studies are primarily based on two-dimensional (2D) cell culture models, which are usually limited in mimicking the real three-dimensional (3D) physiological conditions, cell heterogeneity, cell to cell interaction, and extracellular matrix (ECM) present in living tissues. Traditionally, animal models are used to mimic the 3D environment of tissues and organs, but they suffer from high costs, are time consuming, bring up ethical concerns, and still present many differences when compared to the human body. The applications of microfluidic-based 3D cell culture models are advantageous and useful as they include 3D multicellular model systems (MCMS). These models have demonstrated potential to simulate the in vivo 3D microenvironment with relatively low cost and high throughput. The incorporation of monitoring capabilities in the MCMS has also been explored to evaluate in real time biophysical and chemical parameters of the system, for example temperature, oxygen, pH, and metabolites. Electrochemical sensing is considered as one of the most sensitive and commercially adapted technologies for bio-sensing applications. Amalgamation of electrochemical biosensing with cell culture in microfluidic devices with improved sensitivity and performance are the future of 3D systems. Particularly in cancer, such models with integrated sensing capabilities can be crucial to assess the multiple parameters involved in tumour formation, proliferation, and invasion. In this review, we are focusing on existing 3D cell culture systems with integrated electrochemical sensing for potential applications in cancer models to advance diagnosis and treatment. We discuss their design, sensing principle, and application in the biomedical area to understand the potential relevance of miniaturized electrochemical hybrid systems for the next generation of diagnostic platforms for precision medicine.

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Biomimetic immunomagnetic gold hybrid nanoparticles coupled with inductively coupled plasma mass spectrometry for the detection of circulating tumor cells

Abstract: Immunomagnetic beads are important tools for the isolation and detection of circulating tumor cells (CTCs).

Cited by 22 publications

References 48 publications

Cell‐Released Magnetic Vesicles Capturing Metabolic Labeled Rare Circulating Tumor Cells Based on Bioorthogonal Chemistry

Cell‐Released Magnetic Vesicles Capturing Metabolic Labeled Rare Circulating Tumor Cells Based on Bioorthogonal Chemistry

Cancer nanotechnology: current status and perspectives

Electrochemical Sensing in 3D Cell Culture Models: New Tools for Developing Better Cancer Diagnostics and Treatments

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