Biomimetic human bone marrow tissues: models to study hematopoiesis and platforms for drug testing

Abstract

We propose an in vitro 3D culture system combining perfusion bioreactors, scaffolds and human primary cells to engineer fully-humanized, biomimetic and customizable bone marrow tissues. This system could serve as a model to investigate human hematopoietic stem cell niches, but also as a drug testing platform for pharmaceutical research and patient-personalized medicine.

Control of hydrostatic pressure and osmotic stress in 3D cell culture for mechanobiological studies

“…Integrating this basis with recent breakthroughs in tissue fabrication, biophysics, and biomaterials, should enable bottom-up strategies for the design of bioreactors that are well-suited to investigate the mechanobiology of HP and OS. Novel methods in 3D tissue bioprinting [159][160][161][162][163], mechanical characterization of biological tissues [164][165][166][167][168][169], the formulation of responsive biomaterials with tunable biomechanical and biochemical properties [170][171][172][173][174][175][176], and the development of 3D organotypic cell cultures [177][178][179][180][181][182] highlight a battery of innovations that can be exploited towards the design of advanced materials systems and bioreactors for the control of HP and OS.…”