Biomimetic growth of apatite on hydrogen-implanted silicon

Liu, Xuanyong; Fu, Ricky K.Y.; Poon, R.W.Y.; Chen, Peng; Chu, Paul K.; Ding, Chuanxian

doi:10.1016/j.biomaterials.2004.01.015

Cited by 39 publications

(14 citation statements)

References 24 publications

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“…Therefore, the principal finding is that the nanoporous silicon particles used in this study are capable of forming a calcium phosphate layer at their surface which implies that a direct bond with bone tissue would be possible in vivo. The bioactivity of these particles may be further improved by oxidising the PS samples before SBF immersion or by coating or implanting hydrogen into the silicon particle surface and examining after exposure to SBF [28]. These experiments and exploration of the interfacial layer between the HA and silicon particle surface is part of further work to be pursued and will be promulgated in due course.…”

Section: Tem Analysis After Sbfmentioning

confidence: 99%

Hydroxyapatite formation on metallurgical grade nanoporous silicon particles

2010

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Studies into bone-like apatite or hydroxyapatite (HA) growth on potential biomaterials when in contact with simulated body fluid (SBF) not only establish a general method for determining bioactivity but coincidently lead to the design of new bioactive materials in biomedical and tissue engineering fields. Previous studies of HA growth on porous silicon have examined electrochemically etched silicon substrates after immersion in a simulated body fluid. This study differs from previous work in that it focuses on characterising HA growth on chemically etched metallurgical grade nanoporous silicon particles. The porous silicon (PS) used in this study is comprised of nanosponge particles with disordered pore structures with pore sizes ranging up to 10nm on micron sized particles.The silicon particles are analysed before and after immersion into SBF using scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive x-ray (EDX) analysis and x-ray photoelectron spectroscopy (XPS). Results indicate that a HA layer forms on the surface of the nanosponge particles. Experimental analysis indicates that the morphology and calcium-tophosphorus ratio (Ca/P) verify the formation of crystalline HA on the nanoporous silicon particles.2

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Section: Tem Analysis After Sbfmentioning

confidence: 99%

Hydroxyapatite formation on metallurgical grade nanoporous silicon particles

2010

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“…Amine‐, carboxyl‐, and hydroxyl‐terminal silanes were grafted onto a fresh silicon oxide surface and it was found that the functionalized surface did induce CaP precipitation 13. In our previous work,14, 15 we have found that the bioactivity of silicon wafers was enhanced by hydrogen plasma immersion ion implantation and supposed that the formed amorphous hydrated silica layer which formed by reaction between water molecules and the SiH bonds or Si dangling bonds played a synergistic role to improve the bioactivity.…”

Section: Introductionmentioning

confidence: 98%

Bioactivity and cytocompatibility of silicon wafers treated by water

Niu

Liu

Ding

2007

J Biomedical Materials Res

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A range of bioactive ceramics can induce bone-like apatite to deposit on their surface in simulated body fluid (SBF). In this work, the silicon wafer was treated using deionized water to improve its bioactivity. The morphology and chemical composition of the treated silicon wafer was examined using Fourier transform infrared spectroscopy, atomic force microscopy and X-ray photoelectron spectroscopy. Field emission scanning electron microscopy was used to observe the surface morphologies of silicon wafers soaked in SBF. The results indicated that a hydrated sub-oxide film having Si--OH groups formed on the surface of the silicon wafer after the water treatment. The amount of Si--OH groups increased with raising the treatment temperature or prolonging the immersion time. Apatite was deposited on the surface of water-treated silicon wafers immersed in SBF. The apatite deposition was correlated with the amount of Si--OH groups. Human mesenchymal stem cells cultured on the surface of the water-treated silicon wafers showed good adhesion and spread, indicating that the cytocompatibility of silicon wafer was enhanced by this water treatment.

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“…Furthermore, these PEGDA hydrogel patterns can be positioned anywhere on a hydrogenated amorphous silicon (a-Si:H) substrate using projected images, acting as nonbiofouling blocks to control cell growth. The a-Si:H film (deposited on the top surface of an OEK chip) promotes cellular attachment, 30 especially for mammalian cells because it presents a charged hydrophilic surface in a liquid. 20 We further leverage these characteristic to successfully and reproducibly cluster MCF-7 cells within arbitrarily-defined hydrogel patterns.…”

Section: Introductionmentioning

confidence: 99%

Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics

et al. 2014

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The culturing of cancer cells on micropatterned substrates can provide insight into the factors of the extracellular environment that enable the control of cell growth. We report here a novel non-UV-based technique to quickly micropattern a poly-(ethylene) glycol diacrylate (PEGDA)-based hydrogel on top of modified glass substrates, which were then used to control the growth patterns of breast cancer cells. Previously, the fabrication of micropatterned substrates required relatively complicated steps, which made it impractical for researchers to rapidly and systematically investigate the effects of different cell growth patterns. The technique presented herein operates on the principle of optically-induced electrokinetics (OEKs) and uses computer-generated projection light patterns to dynamically pattern the hydrogel on a hydrogenated amorphous silicon (a-Si:H) thin-film, atop an indium tin oxide (ITO) glass substrate. This technique allows us to pattern lines, circles, pentagons, and more complex shapes in the hydrogel with line widths below 3 μm and thicknesses of up to 6 μm within 8 s by simply controlling the projected illumination pattern and applying an appropriate AC voltage between the two ITO glass substrates. After separating the glass substrates to expose the patterned hydrogel, we experimentally demonstrate that MCF-7 breast cancer cells will adhere to the bare a-Si:H surface, but not to the hydrogel patterned in various geometric shapes and sizes. Theoretical analysis and finite-element model simulations reveal that the dominant OEK forces in our technique are the dielectrophoresis (DEP) force and the electro-osmosis force, which enhance the photo-initiated cross-linking reaction in the hydrogel. Our preliminary cultures of breast cancer cells demonstrate that this reported technique could be applied to effectively confine the growth of cancer cells on a-Si:H surfaces and affect individual cell geometry during their growth.

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Biomimetic growth of apatite on hydrogen-implanted silicon

Cited by 39 publications

References 24 publications

Hydroxyapatite formation on metallurgical grade nanoporous silicon particles

Hydroxyapatite formation on metallurgical grade nanoporous silicon particles

Bioactivity and cytocompatibility of silicon wafers treated by water

Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics

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