Biomimetic glycosaminoglycan-based scaffolds improve skeletal muscle regeneration in a Murine volumetric muscle loss model

Narayanan, Narayanan; Jia, Zhihao; Kim, Kun Ho; Kuang, Liangju; Lengemann, Paul; Shafer, Gabrielle; Bernal-Crespo, Victor; Kuang, Shihuan; Deng, Meng

doi:10.1016/j.bioactmat.2020.10.012

Cited by 33 publications

(30 citation statements)

References 46 publications

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“… 61 In a parallel fashion, hyaluronic acid hydrogels conjugated with chondroitin sulfate (i.e., important components of skeletal muscle tissue ECM) were found to support myoblast differentiation and proper integration with the surrounding host tissue upon 4-week implantation. 62 …”

Section: Hydrogels For Smtementioning

confidence: 99%

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Volpi

Paradiso

Costantini

et al. 2022

ACS Biomater. Sci. Eng.

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The functional capabilities of skeletal muscle are strongly correlated with its well-arranged microstructure, consisting of parallelly aligned myotubes. In case of extensive muscle loss, the endogenous regenerative capacity is hindered by scar tissue formation, which compromises the native muscle structure, ultimately leading to severe functional impairment. To address such an issue, skeletal muscle tissue engineering (SMTE) attempts to fabricate in vitro bioartificial muscle tissue constructs to assist and accelerate the regeneration process. Due to its dynamic nature, SMTE strategies must employ suitable biomaterials (combined with muscle progenitors) and proper 3D architectures. In light of this, 3D fiber-based strategies are gaining increasing interest for the generation of hydrogel microfibers as advanced skeletal muscle constructs. Indeed, hydrogels possess exceptional biomimetic properties, while the fiber-shaped morphology allows for the creation of geometrical cues to guarantee proper myoblast alignment. In this review, we summarize commonly used hydrogels in SMTE and their main properties, and we discuss the first efforts to engineer hydrogels to guide myoblast anisotropic orientation. Then, we focus on presenting the main hydrogel fiber-based techniques for SMTE, including molding, electrospinning, 3D bioprinting, extrusion, and microfluidic spinning. Furthermore, we describe the effect of external stimulation (i.e., mechanical and electrical) on such constructs and the application of hydrogel fiber-based methods on recapitulating complex skeletal muscle tissue interfaces. Finally, we discuss the future developments in the application of hydrogel microfibers for SMTE.

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Section: Hydrogels For Smtementioning

confidence: 99%

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Volpi

Paradiso

Costantini

et al. 2022

ACS Biomater. Sci. Eng.

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“…This hydrogel containing the C2C12 cells helped with muscle regeneration as they were still able to proliferate and promote skeletal muscle regeneration as seen in Figure 5A [76,82]. Another major example of cell delivery for skeletal muscle regeneration was the hyaluronic acid-chitosan (HA-CS) hydrogel system that encapsulated C2C12 cells as well [80]. This hydrogel system aided in the regeneration of myofibers and was able to help with blood vessels and nerve formations in the four weeks that it was implanted into mice [80].…”

Section: Skeletal Muscle Regenerationmentioning

confidence: 99%

“…Another major example of cell delivery for skeletal muscle regeneration was the hyaluronic acid-chitosan (HA-CS) hydrogel system that encapsulated C2C12 cells as well [80]. This hydrogel system aided in the regeneration of myofibers and was able to help with blood vessels and nerve formations in the four weeks that it was implanted into mice [80].…”

Section: Skeletal Muscle Regenerationmentioning

confidence: 99%

Recent Progress in Biopolymer-Based Hydrogel Materials for Biomedical Applications

Mahmood

Patel

Hickson

et al. 2022

IJMS

118

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Hydrogels from biopolymers are readily synthesized, can possess various characteristics for different applications, and have been widely used in biomedicine to help with patient treatments and outcomes. Polysaccharides, polypeptides, and nucleic acids can be produced into hydrogels, each for unique purposes depending on their qualities. Examples of polypeptide hydrogels include collagen, gelatin, and elastin, and polysaccharide hydrogels include alginate, cellulose, and glycosaminoglycan. Many different theories have been formulated to research hydrogels, which include Flory-Rehner theory, Rubber Elasticity Theory, and the calculation of porosity and pore size. All these theories take into consideration enthalpy, entropy, and other thermodynamic variables so that the structure and pore sizes of hydrogels can be formulated. Hydrogels can be fabricated in a straightforward process using a homogeneous mixture of different chemicals, depending on the intended purpose of the gel. Different types of hydrogels exist which include pH-sensitive gels, thermogels, electro-sensitive gels, and light-sensitive gels and each has its unique biomedical applications including structural capabilities, regenerative repair, or drug delivery. Major biopolymer-based hydrogels used for cell delivery include encapsulated skeletal muscle cells, osteochondral muscle cells, and stem cells being delivered to desired locations for tissue regeneration. Some examples of hydrogels used for drug and biomolecule delivery include insulin encapsulated hydrogels and hydrogels that encompass cancer drugs for desired controlled release. This review summarizes these newly developed biopolymer-based hydrogel materials that have been mainly made since 2015 and have shown to work and present more avenues for advanced medical applications.

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“…A number of studies have also demonstrated that heparin has the ability to sequester and release exogenously added growth factors to ultimately improve wound healing and tissue regeneration. − In the context of muscular regeneration, heparin mimetics have demonstrated a capacity to stimulate muscle repair, which is thought to be mediated by the enhanced bioavailability of endogenously released heparin binding growth factors . A range of hydrogels based on HyA have previously been used with applications to VML repair in mind, although it should be noted that in comparison with this study, some of these HyA hydrogels were used/designed to deliver progenitor cells. − Combined, the potential relevance of HyA hydrogels to skeletal muscle regeneration is apparent, and furthermore, these materials have been shown to significantly improve donor cell survival after transplantation …”

Section: Introductionmentioning

confidence: 96%

Semisynthetic Hyaluronic Acid-Based Hydrogel Promotes Recovery of the Injured Tibialis Anterior Skeletal Muscle Form and Function

Dienes

Browne

Farjun

et al. 2021

ACS Biomater. Sci. Eng.

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Volumetric muscle loss (VML) injuries are characterized by a degree of tissue loss that exceeds the endogenous regenerative capacity of muscle, resulting in permanent structural and functional deficits. Such injuries are a consequence of trauma, as well as a host of congenital and acquired diseases and disorders. Despite significant preclinical research with diverse biomaterials, as well as early clinical studies with implantation of decellularized extracellular matrices, there are still significant barriers to more complete restoration of muscle form and function following repair of VML injuries. In fact, identification of novel biomaterials with more advantageous regenerative profiles is a critical limitation to the development of improved therapeutics. As a first step in this direction, we evaluated a novel semisynthetic hyaluronic acid-based (HyA) hydrogel that embodies material features more favorable for robust muscle regeneration. This HyA-based hydrogel is composed of an acrylate-modified HyA (AcHyA) macromer, an AcHyA macromer conjugated with the bsp-RGD(15) peptide sequence to enhance cell adhesion, a high-molecular-weight heparin to sequester growth factors, and a matrix metalloproteinase-cleavable cross-linker to allow for cell-dependent remodeling. In a well-established, clinically relevant rat tibialis anterior VML injury model, we report observations of robust functional recovery, accompanied by volume reconstitution, muscle regeneration, and native-like vascularization following implantation of the HyA-based hydrogel at the site of injury. These findings have important implications for the development and clinical application of the improved biomaterials that will be required for stable and complete functional recovery from diverse VML injuries.

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Biomimetic glycosaminoglycan-based scaffolds improve skeletal muscle regeneration in a Murine volumetric muscle loss model

Cited by 33 publications

References 46 publications

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Recent Progress in Biopolymer-Based Hydrogel Materials for Biomedical Applications

Semisynthetic Hyaluronic Acid-Based Hydrogel Promotes Recovery of the Injured Tibialis Anterior Skeletal Muscle Form and Function

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