Biomimetic glycopeptide hydrogel coated PCL/nHA scaffold for enhanced cranial bone regeneration via macrophage M2 polarization-induced osteo-immunomodulation

Wang, Yaping; Wang, Jingrong; Gao, Rui; Liu, Xiang; Feng, Zujian; Zhang, Chuangnian; Huang, Pingsheng; Dong, Anjie; Kong, Deling; Wang, Weiwei

doi:10.1016/j.biomaterials.2022.121538

Cited by 87 publications

(62 citation statements)

References 52 publications

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“…Typically, bone defect healing is one of the most complicated physiological processes in the human body. It requires a well-orchestrated series of biological events, including initial immunomodulation, vascular ingrowth, osteogenic differentiation, and biomineralization . Various factors, including the bone matrix, bone marrow, and periosteum, have been found to participate in these processes for interaction and cooperation.…”

Section: Introductionmentioning

confidence: 99%

Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

Liu

Shi

Zhu

et al. 2023

ACS Appl. Mater. Interfaces

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Ideal periosteum materials are required to participate in a sequence of bone repair-related physiological events, including the initial immune response, endogenous stem cell recruitment, angiogenesis, and osteogenesis. However, conventional tissue-engineered periosteal materials have difficulty achieving these functions by simply mimicking the periosteum via structural design or by loading exogenous stem cells, cytokines, or growth factors. Herein, we present a novel biomimetic periosteum preparation strategy to comprehensively enhance the bone regeneration effect using functionalized piezoelectric materials. The resulting biomimetic periosteum possessing an excellent piezoelectric effect and improved physicochemical properties was prepared using a biocompatible and biodegradable poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) polymer matrix, antioxidized polydopamine-modified hydroxyapatite (PHA), and barium titanate (PBT), which were further incorporated into the polymer matrix to fabricate a multifunctional piezoelectric periosteum by a simple one-step spin-coating method. The addition of PHA and PBT dramatically enhanced the physicochemical properties and biological functions of the piezoelectric periosteum, resulting in improved surface hydrophilicity and roughness, enhanced mechanical performance, tunable degradation behavior, and stable and desired endogenous electrical stimulations, which is conducive to accelerating bone regeneration. Benefiting from endogenous piezoelectric stimulation and bioactive components, the as-fabricated biomimetic periosteum demonstrated favorable biocompatibility, osteogenic activity, and immunomodulatory functions in vitro, which not only promoted adhesion, proliferation, and spreading as well as osteogenesis of mesenchymal stem cells (MSCs) but also effectively induced M2 macrophage polarization, thereby suppressing reactive oxygen species (ROS)-induced inflammatory reactions. Through in vivo experiments, the biomimetic periosteum with endogenous piezoelectric stimulation synergistically accelerated the formation of new bone in a rat critical-sized cranial defect model. The whole defect was almost completely covered by new bone at 8 weeks post treatment, with a thickness close to that of the host bone. Collectively, with its favorable immunomodulatory and osteogenic properties, the biomimetic periosteum developed here represents a novel method to rapidly regenerate bone tissue using piezoelectric stimulation.

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Section: Introductionmentioning

confidence: 99%

Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

Liu

Shi

Zhu

et al. 2023

ACS Appl. Mater. Interfaces

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“…For example, reduced scale honeycomb-like TiO2 structures were shown to facilitate macrophage filopodia formation to reinforce its polarization toward M2, which further triggered osteogenic differentiation of bone mesenchymal stem cells (BMSCs). [14] Many hydrogels, such as GelMA hydrogel, [15] carboxymethyl chitosan/poly(ethylene glycol) diacrylate hydrogel, [16] glycopeptide hydrogel, [17] and chitosan hydrogel, [18] have also been modified and used to promote bone repair through facilitating M2 macrophage polarization. However, as the pro-regenerative role of M2 macrophages was overly emphasized in these efforts, the positive effect of M1 macrophages in terms of damage-associated debris clearance, immune cell recruitment, and angiogenic induction was underestimated, which may severely compromise the effectiveness of such immunomodulatory bone healing strategies.…”

Section: Introductionmentioning

confidence: 99%

Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

Huang

et al. 2022

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Precise timing of macrophage polarization plays a pivotal role in immunomodulation of tissue regeneration, yet most studies mainly focus on M2 macrophages for their anti‐inflammatory and regenerative effects while the essential proinflammatory role of the M1 phenotype on the early inflammation stage is largely underestimated. Herein, a superparamagnetic hydrogel capable of timely controlling macrophage polarization is constructed by grafting superparamagnetic nanoparticles on collagen nanofibers. The magnetic responsive hydrogel network enables efficient polarization of encapsulated macrophage to the M2 phenotype through the podosome/Rho/ROCK mechanical pathway in response to static magnetic field (MF) as needed. Taking advantage of remote accessibility of magnetic field together with the superparamagnetic hydrogels, a temporal engineered M1 to M2 transition course preserving the essential role of M1 at the early stage of tissue healing, as well as enhancing the prohealing effect of M2 at the middle/late stages is established via delayed MF switch. Such precise timing of macrophage polarization matching the regenerative process of injured tissue eventually leads to optimized immunomodulatory bone healing in vivo. Overall, this study offers a remotely time‐scheduled approach for macrophage polarization, which enables precise manipulation of inflammation progression during tissue healing.

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“…The in situ forming ability and plasticity of hydrogels enable the aesthetic repair of craniomaxillofacial bone defects. The high hydration and interlinked network of hydrogels give them good biocompatibility and physical properties, making hydrogels an excellent vehicle for transporting cells [ 15 , 16 , 17 , 18 ]. In addition, one of the major advantages of hydrogels is that their composition can be modified to obtain drug-carrying properties for different types of drugs.…”

Section: Hydrogels: Distinctive Properties For Craniomaxillofacial Bo...mentioning

confidence: 99%

“…Furthermore, as previously mentioned, BMP-2 can be firmly tethered to the hydrogel surface by covalent bonds, and hydrogels can be modified on their surfaces using their abundant side chain groups to achieve surface bionic camouflage to accurately mimic the healing environment of bone defects. A glycopeptide hydrogel (GRgel) formed by β-sheet RADA16-grafted glucomannan self-assembly was developed by Wang et al, for the formation of a bionic camouflage surface of polycaprolactone/nHA (PCL/nHA) scaffold for good cranial repair [ 16 ].…”

Section: Smart Hydrogels: Application In Craniomaxillofacial Bone Eng...mentioning

confidence: 99%

Functional Hydrogels and Their Applications in Craniomaxillofacial Bone Regeneration

Wang

et al. 2022

Pharmaceutics

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Craniomaxillofacial bone defects are characterized by an irregular shape, bacterial and inflammatory environment, aesthetic requirements, and the need for the functional recovery of oral–maxillofacial areas. Conventional clinical treatments are currently unable to achieve high-quality craniomaxillofacial bone regeneration. Hydrogels are a class of multifunctional platforms made of polymers cross-linked with high water content, good biocompatibility, and adjustable physicochemical properties for the intelligent delivery of goods. These characteristics make hydrogel systems a bright prospect for clinical applications in craniomaxillofacial bone. In this review, we briefly demonstrate the properties of hydrogel systems that can come into effect in the field of bone regeneration. In addition, we summarize the hydrogel systems that have been developed for craniomaxillofacial bone regeneration in recent years. Finally, we also discuss the prospects in the field of craniomaxillofacial bone tissue engineering; these discussions can serve as an inspiration for future hydrogel design.

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Biomimetic glycopeptide hydrogel coated PCL/nHA scaffold for enhanced cranial bone regeneration via macrophage M2 polarization-induced osteo-immunomodulation

Cited by 87 publications

References 52 publications

Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

Functional Hydrogels and Their Applications in Craniomaxillofacial Bone Regeneration

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