Biomimetic collagen/elastin meshes for ventral hernia repair in a rat model

Minardi, Silvia; Taraballi, Francesca; Wang, Xin; Cabrera, Fernando; Eps, Jeffrey L. Van; Robbins, Andrew B.; Sandri, Monica; Moreno, Michael R.; Weiner, Bradley K.; Tasciotti, Ennio

doi:10.1016/j.actbio.2016.11.032

Cited by 46 publications

(35 citation statements)

References 56 publications

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“…Biomimetic meshes could be very usefully for the repair of different tissues. We recently demonstrated that could be a valid alternative to the clinical polymeric materials used in soft tissue repair (Minardi et al, 2016c ) as well as in cell delivery for more regenerative medicine approaches (Pandolfi et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

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Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes

Corradetti

Taraballi

Giretti

et al. 2017

Front. Bioeng. Biotechnol.

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Clinical trials have demonstrated that heparan sulfate (HS) could be used as a therapeutic agent for the treatment of inflammatory diseases. Its anti-inflammatory effect makes it suitable for the development of biomimetic innovative strategies aiming at modulating stem cells behavior toward a pro-regenerative phenotype in case of injury or inflammation. Here, we propose collagen type I meshes fabricated by solvent casting and further crosslinked with HS (HS-Col) to create a biomimetic environment resembling the extracellular matrix of soft tissue. HS-Col meshes were tested for their capability to provide physical support to stem cells’ growth, maintain their phenotypes and immunosuppressive potential following inflammation. HS-Col effect on stem cells was investigated in standard conditions as well as in an inflammatory environment recapitulated in vitro through a mix of pro-inflammatory cytokines (tumor necrosis factor-α and interferon-gamma; 20 ng/ml). A significant increase in the production of molecules associated with immunosuppression was demonstrated in response to the material and when cells were grown in presence of pro-inflammatory stimuli, compared to bare collagen membranes (Col), leading to a greater inhibitory potential when mesenchymal stem cells were exposed to stimulated peripheral blood mononuclear cells. Our data suggest that the presence of HS is able to activate the molecular machinery responsible for the release of anti-inflammatory cytokines, potentially leading to a faster resolution of inflammation.

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Section: Discussionmentioning

confidence: 99%

“…Type I collagen mesh (Col) from bovine tendon (5% collagen gel in acetic acid) was produced by a solvent-casting method as previously described (Taraballi et al, 2013 ; Minardi et al, 2016c ). Briefly, 1 g of type I collagen (Nitta Casings Inc.) was dissolved in an acetate buffer (pH 3.5) at the final concentration of 20 mg/ml.…”

Section: Methodsmentioning

confidence: 99%

Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes

Corradetti

Taraballi

Giretti

et al. 2017

Front. Bioeng. Biotechnol.

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“…The type of interaction of both proteins can start from a simple approach such as blending and casting at room temperature or freezedrying to induce crosslinking, until the use of crosslinkers such as genipin, or via the 1-Ethyl-3-(3dimethylaminopropyl)carbodiimide/Nhydroxysuccinimide (EDC/NHS) route (66). For scaffolds that require a higher mechanical response, stability, and resistance to degradation, chemical crosslinking is a suitable alternative to improve their performance (67). Although the freeze-drying technique induces pore formation during the sublimation process, the control of tortuosity can be increased and enhanced by injection of CO2 at controlled pressure and temperature (68).…”

Section: Scaffoldsmentioning

confidence: 99%

Recent Advances in Elastin-Based Biomaterials

et al. 2020

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Elastin is one of the main components of the extracellular matrix; it provides resistance and elasticity to a variety of tissues and organs of the human body, besides participating in cellular signaling. On the other hand, elastin-derived peptides are synthetic biopolymers with a similar conformation and structure to elastin, but these possess the advantage of solubility in aqueous mediums. Due to their biological activities and physicochemical properties, elastin and related peptides may be applied as biomaterials to develop diverse biomedical devices, including scaffolds, hydrogels, and drug delivery systems for tissue engineering. Likewise, the combination of elastin with natural or synthetic polymers has demonstrated to improve the mechanical properties of biomedical products and drug delivery systems. Here we comprehensively describe the physicochemical properties and physiological functions of elastin. Moreover, we offer an overview of the use of elastin and its derivative polymers as biomaterials to develop scaffolds and hydrogels for tissue engineering. Finally, we discuss some perspectives on the employment of these biopolymers to fabricate new biomedical products.

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“…The in vitro characterization showed that these meshes maintained human MSC survival, proliferation as well as cell stemness features for up to 3 weeks. Importantly, the meshes implanted in vivo prevented the recurrence of ventral hernias in all animals, and histological analysis revealed high vascularization and full integration of the meshes within the host tissue (Minardi et al, ). Another study showed that PLA/PCL electruspun nanofibrous scaffolds supported osteogenic differentiation of human MSC in vitro and promoted new bone tissue formation also inside the three‐dimension scaffolds when implanted into a mouse model of critical‐sized cranial bone defect (Q. Yao et al, ).…”

Section: Tissue Engineering and Biomechanicsmentioning

confidence: 99%

Role of substrate biomechanics in controlling (stem) cell fate: Implications in regenerative medicine

Macri‐Pellizzeri

De‐Juan‐Pardo

Prósper

et al. 2017

J Tissue Eng Regen Med

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Tissue-specific stem cells reside in a specialized environment known as niche. The niche plays a central role in the regulation of cell behaviour and, through the concerted action of soluble molecules, supportive somatic cells, and extracellular matrix components, directs stem cells to proliferate, differentiate, or remain quiescent. Great efforts have been done to decompose and separately analyse the contribution of these cues in the in vivo environment. Specifically, the mechanical properties of the extracellular matrix influence many aspects of cell behaviour, including self-renewal and differentiation. Deciphering the role of biomechanics could thereby provide important insights to control the stem cells responses in a more effective way with the aim to promote their therapeutic potential. In this review, we provide a wide overview of the effect that the microenvironment stiffness exerts on the control of cell behaviour with a particular focus on the induction of stem cells differentiation. We also describe the process of mechanotransduction and the molecular effectors involved. Finally, we critically discuss the potential involvement of tissue biomechanics in the design of novel tissue engineering strategies.

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Biomimetic collagen/elastin meshes for ventral hernia repair in a rat model

Cited by 46 publications

References 56 publications

Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes

Heparan Sulfate: A Potential Candidate for the Development of Biomimetic Immunomodulatory Membranes

Recent Advances in Elastin-Based Biomaterials

Role of substrate biomechanics in controlling (stem) cell fate: Implications in regenerative medicine

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