Biomimetic calcium-inactivated ion/molecular channel

Li, Minmin; Cao, Yuchen; Zhang, Xin; Wang, Dongdong; Qian, Shengxu; Li, Guodong; Zhang, Fusheng; Xiong, Yüting; Qing, Guangyan

doi:10.1039/d1cc03058b

Cited by 14 publications

(9 citation statements)

References 35 publications

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“…[ 1,2 ] However, the main absorption spectra of these photosensitizers are still located in the visible region <700 nm, which largely attenuates the therapeutic effect in deep tissues because of the intense light absorption and scattering within biological tissues. [ 24 ] Near‐infrared (NIR) light in the biological transparency window of 700–1000 nm displays deeper body penetration and minimal tissue absorption, [ 25–29 ] and thus much attention has been recently focused on developing NIR photosensitizers for deep PDT treatment, including boron dipyrromethene (BODIPY) molecules, [ 30–32 ] cyanine derivatives, [ 15,16,33–36 ] metal‐organic complexes, [ 37,38 ] and aggregation‐induced emission (AIE) compounds. [ 39–41 ] Nevertheless, these BODIPY and metal‐organic complexes usually contain heavy atoms such as I, Br, Ru, and Ir to boost singlet oxygen ( 1 O 2 ) generation, [ 31,32,37,38 ] resulting in obvious dark toxicity, low fluorescence, and tedious synthesis.…”

Section: Introductionmentioning

confidence: 99%

Three Birds with One Stone: Acceptor Engineering of Hemicyanine Dye with NIR‐II Emission for Synergistic Photodynamic and Photothermal Anticancer Therapy

Cao

Zhu

et al. 2022

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It is challenging to develop a near‐infrared (NIR) small molecular photosensitizer for synergistic phototherapy in deep tissues. Herein, first, a heavy‐atom‐free NIR hemicyanine photosensitizer (BHcy) for 808 nm light‐mediated synergistic photodynamic therapy/photothermal therapy (PDT/PTT) anticancer therapy by leveraging the acceptor engineering strategy is reported. This strategy endows BHcy with a more planar and larger π‐conjugated structure, resulting in long NIR absorption/emission at 770/915–1200 nm as well as enhanced singlet oxygen (1O2) generation ability and photothermal effect, which is ascribed to the reduced energy levels of excited singlet/triplet states and the promoted intersystem crossing process. Notably, BHcy‐based nanoparticles (BHcy‐NPs) exhibit efficient 1O2 yield (12.9%) and high photothermal conversion efficiency (55.1%). More importantly, BHcy‐NPs are able to significantly kill cancer cells by destroying main organelles and inhibit tumor growth in vivo after a single irradiation. Overall, this study provides a strategy to design new heavy‐atom‐free PDT/PTT agents for potential clinical applications.

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Section: Introductionmentioning

confidence: 99%

Three Birds with One Stone: Acceptor Engineering of Hemicyanine Dye with NIR‐II Emission for Synergistic Photodynamic and Photothermal Anticancer Therapy

Cao

Zhu

et al. 2022

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“…Then, the surface charge density of the TSC nanochannel inner wall was investigated by measuring the electroosmotic flow velocity, 44,45 which was obtained by recording the transport rate of phenol molecules through multiple TSC nanochannels (Figures S17 and S18). 46,47 Upon treatment with MA8-KMe3, the surface charge density reduced from the initial −0.0135 to −0.0198 e• nm −2 . This further suggests that the negative charge count on the surface of the TSC layer increased following its binding with MA8-KMe3.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Nanofluidic Device for Detection of Lysine Methylpeptides and Sensing of Lysine Methylation

Xiong

Cao

et al. 2023

Anal. Chem.

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Protein methylation is the smallest possible yet vitally important post-translational modification (PTM). This small and chemically inert addition in proteins makes the analysis of methylation more challenging, thus calling for an efficient tool for the sake of recognition and detection. Herein, we present a nanofluidic electric sensing device based on a functionalized nanochannel that was constructed by introducing monotriazole-containing p-sulfonatocalix[4]arene (TSC) into a single asymmetric polymeric nanochannel via click chemistry. The device can selectively detect lysine methylpeptides with subpicomole sensitivity, distinguish between different lysine methylation states, and monitor the lysine methylation process by methyltransferase at the peptide level in real time. The introduced TSC molecule, with its confined asymmetric configuration, presents the remarkable ability to selectively bind to lysine methylpeptides, which, coupled with the release of the complexed Cu ions, allows the device to transform the molecular-level recognition to the discernible change in ionic current of the nanofluidic electric device, thus enabling detection. This work could serve as a stepping stone to the development of a new methyltransferase assay and the chemical that specifically targets lysine methylation in PTM proteomics.

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“…It is well known that the ion transport behavior can be regulated by the wettability and surface charge density of the nanochannels. , Therefore, to explore the sensing mechanism of Tyr-MS/AAO, CA and zeta potential analyses were performed. As shown in Figure a, after TYR (50 U ml –1 , 200 μL) was added to Tyr-MS/AAO, the water CA (θ) decreased from 56.9 ± 2.6° to 53.1 ± 2.0°.…”

Section: Resultsmentioning

confidence: 99%

Interfacially Super-Assembled Tyramine-Modified Mesoporous Silica-Alumina Oxide Heterochannels for Label-Free Tyrosinase Detection

et al. 2021

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Tyrosinase (TYR) is a multifunctional copper-containing enzyme that plays a critical role in the biosynthetic pathway of melanin. Thus, the detection of TYR activity possesses vast importance from clinical diagnosis to the food industry. However, most TYR detection methods are expensive, complicated, and time-consuming. Herein, a functional nanofluidic heterochannel composed of an ultrathin tyramine-modified mesoporous silica layer (Tyr-MS) and alumina oxide (AAO) arrays is constructed by an interfacial super-assembly method. The heterochannel with plenty of enzyme catalytic sites for TYR provides the response of the ion current signal against TYR concentrations. Introducing enzymatic reaction paves the way for the heterochannel to achieve label-free, selective, specific detection of TYR. Notably, a highly sensitive detection of TYR with a limit of 2 U mL–1 was obtained by optimizing the modified conditions. Detailed investigations and theoretical calculations further reveal the mechanism for the detection performance. This work provides a simple, low-cost, quick response, and label-free platform based on functional nanofluidic devices for enzyme-sensing technologies.

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Biomimetic calcium-inactivated ion/molecular channel

Abstract: A phosphopeptide-modified nanochannel was prepared based on a conical polymeric nanopore. It shows a reversible Ca2+-induced inactivation effect toward the ion flow and molecular transport, resulting from Ca2+ binding-caused surface...

Cited by 14 publications

References 35 publications

Three Birds with One Stone: Acceptor Engineering of Hemicyanine Dye with NIR‐II Emission for Synergistic Photodynamic and Photothermal Anticancer Therapy

Three Birds with One Stone: Acceptor Engineering of Hemicyanine Dye with NIR‐II Emission for Synergistic Photodynamic and Photothermal Anticancer Therapy

Nanofluidic Device for Detection of Lysine Methylpeptides and Sensing of Lysine Methylation

Interfacially Super-Assembled Tyramine-Modified Mesoporous Silica-Alumina Oxide Heterochannels for Label-Free Tyrosinase Detection

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