Biomimetic bone regeneration

Hing, Karin A.

doi:10.1533/9780857098887.2.207

Cited by 4 publications

(4 citation statements)

References 137 publications

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“…When designing a scaffold for bone tissue engineering (BTE), one of the most important features to consider is porosity. In natural human bones, porosity allows for the ramification of blood vessels, stores and protects bone marrow, hosts the bone cells (osteocytes) in holes, called lacunae, and connects them through small channels, called canaliculi [ 1 , 2 ]. Porosity also directly affects bones’ mechanical properties, and it is considered by some authors [ 3 ] to be the only difference between cancellous bone (spongy, ~50–90% porosity [ 4 ]) and cortical bone (compact, ~5–10% porosity [ 4 ]).…”

Section: Introductionmentioning

confidence: 99%

Powder 3D Printing of Bone Scaffolds with Uniform and Gradient Pore Sizes Using Cuttlebone-Derived Calcium Phosphate and Glass-Ceramic

et al. 2022

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The pore geometry of bone scaffolds has a major impact on their cellular response; for this reason, 3D printing is an attractive technology for bone tissue engineering, as it allows for the full control and design of the porosity. Calcium phosphate materials synthesized from natural sources have recently attracted a certain interest because of their similarity to natural bone, and they were found to show better bioactivity than synthetic compounds. Nevertheless, these materials are very challenging to be processed by 3D printing due to technological issues related to their nanometric size. In this work, bone scaffolds with different pore geometries, with a uniform size or with a size gradient, were fabricated by binder jetting 3D printing using a biphasic calcium phosphate (BCP) nanopowder derived from cuttlebones. To do so, the nanopowder was mixed with a glass-ceramic powder with a larger particle size (45–100 µm) in 1:10 weight proportions. Pure AP40mod scaffolds were also printed. The sintered scaffolds were shown to be composed mainly by hydroxyapatite (HA) and wollastonite, with the amount of HA being larger when the nanopowder was added because BCP transforms into HA during sintering at 1150 °C. The addition of bio-derived powder increases the porosity from 60% to 70%, with this indicating that the nanoparticles slow down the glass-ceramic densification. Human mesenchymal stem cells were seeded on the scaffolds to test the bioactivity in vitro. The cells’ number and metabolic activity were analyzed after 3, 5 and 10 days of culturing. The cellular behavior was found to be very similar for samples with different pore geometries and compositions. However, while the cell number was constantly increasing, the metabolic activity on the scaffolds with gradient pores and cuttlebone-derived powder decreased over time, which might be a sign of cell differentiation. Generally, all scaffolds promoted fast cell adhesion and proliferation, which were found to penetrate and colonize the 3D porous structure.

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Section: Introductionmentioning

confidence: 99%

Powder 3D Printing of Bone Scaffolds with Uniform and Gradient Pore Sizes Using Cuttlebone-Derived Calcium Phosphate and Glass-Ceramic

et al. 2022

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“…Hydroxyapatite (HA)-based materials have become gold standard bioceramics despite being nondegradable 12 , 13 in vivo. There are other promising materials like α- and β-tricalcium phosphate (TCP), but their resorption is too fast and nonuniform, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Hydroxyapatite (HA)-based materials have become gold standard bioceramics despite being nondegradable , in vivo. There are other promising materials like α- and β-tricalcium phosphate (TCP), but their resorption is too fast and nonuniform, respectively. ,, Dicalcium phosphate dihydrate (DCPD), also known as brushite, is a promising alternative with both good resorbability in vivo and good biocompatibility. , However, brushite materials, similar to other bioceramics, are brittle and prone to in vivo mechanical failure .…”

Section: Introductionmentioning

confidence: 99%

Chiral Tartaric Acid Improves Fracture Toughness of Bioactive Brushite–Collagen Bone Cements

Sarrigiannidis

Moussa

Dobre

et al. 2020

ACS Appl. Bio Mater.

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Brushite cements are promising bone regeneration materials with limited biological and mechanical properties. Here, we engineer a mechanically improved brushite–collagen type I cement with enhanced biological properties by use of chiral chemistry; d - and l -tartaric acid were used to limit crystal growth and increase the mechanical properties of brushite–collagen cements. The impact of the chiral molecules on the cements was examined with Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). A 3-point bend test was utilized to study the fracture toughness, and cell attachment and morphology studies were carried out to demonstrate biocompatibility. XRD and SEM analyses showed that l -, but not d -tartaric acid, significantly restrained brushite crystal growth by binding to the {010} plane of the mineral and increased brushite crystal packing and the collagen interaction area. l -Tartaric acid significantly improved fracture toughness compared to traditional brushite by 30%. Collagen significantly enhanced cell morphology and focal adhesion expression on l -tartaric acid-treated brushite cements.

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“…Com base nesses requisitos, espera-se que os biomateriais implantados apresentem um desempenho similar ao do tecido hospedeiro. A forma como o biomaterial se comporta em meio biológico permite que esses materiais sejam classificados como: bioinertes, biotoleráveis, bioativos e bioreabsorvíveis (HING, 2013).…”

Section: Biomateriaisunclassified

Influência do estrôncio na formação de fosfatos de cálcio sobre a superfície do nanocompósito de Al>sub<2>/sub<O>sub<3>/sub</ZrO>sub<2>/sub<

Nunes¹

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AGRADECIMENTOSA Deus, A minha orientadora, Profa Dra. Eliria M. J. A. Pallone, minha maior incentivadora, referência no meio acadêmico, amiga, e por sempre me indicar os caminhos que devo seguir.A minha coorientadora, Profa Dra. Julieta A. Ferreira, pela parceria, torcida, amizade e pelos puxões de orelha. Sempre me incentivando e me orientando.Aos funcionários do ZEB e da secretaria do Programa de Pós Graduação da FZEA, por toda prestatividade e gentileza.

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Biomimetic bone regeneration

Cited by 4 publications

References 137 publications

Powder 3D Printing of Bone Scaffolds with Uniform and Gradient Pore Sizes Using Cuttlebone-Derived Calcium Phosphate and Glass-Ceramic

Powder 3D Printing of Bone Scaffolds with Uniform and Gradient Pore Sizes Using Cuttlebone-Derived Calcium Phosphate and Glass-Ceramic

Chiral Tartaric Acid Improves Fracture Toughness of Bioactive Brushite–Collagen Bone Cements

Influência do estrôncio na formação de fosfatos de cálcio sobre a superfície do nanocompósito de Al>sub<2>/sub<O>sub<3>/sub</ZrO>sub<2>/sub<

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