Biomimetic Biphasic Electrospun Scaffold for Anterior Cruciate Ligament Tissue Engineering

Tang, Ya Hui; Tian, Jialiang; Li, Long; Huang, Lin; Shen, Quan; Guo, Shanzhu; Jiang, Yue

doi:10.1007/s13770-021-00376-7

Cited by 12 publications

(8 citation statements)

References 34 publications

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“…They found that PLGA/PCL (1:5) fiber scaffolds had the highest ultimate tensile strength (58.3 ± 7.4 MPa) and elongation at break (67% ± 1.8%). [ 157 ] In a study by Kahn et al., the mechanical properties of poly( l ‐lactic‐ co ‐glycolic acid)‐poly( l ‐acid‐ co ‐caprolactone) (PLGA‐PLCL) were investigated. The study found that after 21 days, Young's modulus of the scaffolds decreased from 14–15 to 11.3 MPa due to the hydrolytic degradation of PLGA.…”

Section: Different Materials Scaffoldsmentioning

confidence: 99%

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Advancements in scaffold for treating ligament injuries; in vitro evaluation

Liu,

Al‐Danakh,

Wang

et al. 2023

Biotechnology Journal

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Tendon/ligament (T/L) injuries are a worldwide health problem that affects millions of people annually. Due to the characteristics of tendons, the natural rehabilitation of their injuries is a very complex and lengthy process.Surgical treatment of a T/L injury frequently necessitates using autologous or allogeneic grafts or synthetic materials.Nonetheless, these alternatives have limitations in terms of mechanical properties and histocompatibility, and they do not permit the restoration of the original biological function of the tissue, which can negatively impact the patient's quality of life. It is crucial to find biological materials that possess the necessary properties for the successful surgical treatment of tissues and organs. In recent years, the in vitro regeneration of tissues and organs from stem cells has emerged as a promising approach for preparing autologous tissue and organs, and cell culture scaffolds play a critical role in this process. However, the biological traits and serviceability of different materials used for cell culture scaffolds vary significantly, which can impact the properties of the cultured tissues. Therefore, this review aims to analyze the differences in the biological properties and suitability of various materials based on scaffold characteristics such as cell compatibility, degradability, textile technologies, fiber arrangement, pore size, and porosity. This comprehensive analysis provides valuable insights to aid in the selection of appropriate scaffolds for in vitro tissue and organ culture.This article is protected by copyright. All rights reserved

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Section: Different Materials Scaffoldsmentioning

confidence: 99%

“…Positive results post-implantation. [157] PLGA nanofiber membranes Created scaffolds with doxycycline, collagen, and bupivacaine.…”

Section: Refmentioning

confidence: 99%

Advancements in scaffold for treating ligament injuries; in vitro evaluation

Liu,

Al‐Danakh,

Wang

et al. 2023

Biotechnology Journal

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“…Among these three elements, scaffolds play a significant role, as they provide an artificial microenvironment for cell growth. Recent advances in tissue engineering have focused on the development of biomimetic scaffolds that simulate the extracellular matrix (ECM) of native tissues regarding both structure and composition [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Topographic Orientation of Scaffolds for Tissue Regeneration: Recent Advances in Biomaterial Design and Applications

et al. 2022

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Tissue engineering to develop alternatives for the maintenance, restoration, or enhancement of injured tissues and organs is gaining more and more attention. In tissue engineering, the scaffold used is one of the most critical elements. Its characteristics are expected to mimic the native extracellular matrix and its unique topographical structures. Recently, the topographies of scaffolds have received increasing attention, not least because different topographies, such as aligned and random, have different repair effects on various tissues. In this review, we have focused on various technologies (electrospinning, directional freeze-drying, magnetic freeze-casting, etching, and 3-D printing) to fabricate scaffolds with different topographic orientations, as well as discussed the physicochemical (mechanical properties, porosity, hydrophilicity, and degradation) and biological properties (morphology, distribution, adhesion, proliferation, and migration) of different topographies. Subsequently, we have compiled the effect of scaffold orientation on the regeneration of vessels, skin, neural tissue, bone, articular cartilage, ligaments, tendons, cardiac tissue, corneas, skeletal muscle, and smooth muscle. The compiled information in this review will facilitate the future development of optimal topographical scaffolds for the regeneration of certain tissues. In the majority of tissues, aligned scaffolds are more suitable than random scaffolds for tissue repair and regeneration. The underlying mechanism explaining the various effects of aligned and random orientation might be the differences in “contact guidance”, which stimulate certain biological responses in cells.

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“…Previous studies showed that the surface roughness of biomaterials influenced cell adhesion and focal contact formation. [ 23–26 ]…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies showed that the surface roughness of biomaterials influenced cell adhesion and focal contact formation. [23][24][25][26] In this study, we hypothesized that differences in cell-fiber interactions related to surface topography and chemical composition of fibers would affect the diffusion of nutrients or oxygen within composite spheroids to determine cell viability and proliferation. To test our hypothesis, polycaprolactone (PCL) fibers with different surface porosity and roughness were prepared through the control of relative humidity during electrospinning, and changes in chemical composition were achieved by epigallocatechin gallate (EGCG) coating.…”

Section: Introductionmentioning

confidence: 99%

Composite Multicellular Spheroids Containing Fibers with Pores and Epigallocatechin Gallate (EGCG) Coating on the Surface for Enhanced Proliferation of Stem Cells

Lee

Choi

Byun

et al. 2022

Macromolecular Bioscience

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Multicellular spheroids are formed by strong cell-cell and cell-extracellular matrix interactions and are widely utilized in tissue engineering for therapeutic treatments or ex vivo tissue modeling. However, diffusion of oxygen into the spheroid gradually decreases, forming a necrotic core. In this study, polycaprolactone (PCL) fibers with pores and epigallocatechin gallate (EGCG) coating on their surface to provide a structural framework within the spheroids and investigated their ability to mitigate diffusional limitation and control over the proliferation of human adipose-derived stem cells (hADSCs) is engineered. The DNA content of composite spheroids prepared from fibers and hADSCs decreased in unadjusted cells (1224 ± 134 ng), in those with fibers with a smooth surface (SF) (1447 ± 331 ng), and in those EGCG-coated with SF (E-SF) (1437 ± 289 ng). Cells with fibers with pores on the surface (PF) (2020 ± 32 ng) and those with EGCG-coated PF (E-PF) (1911 ± 80 ng) increased after 7 days of culture, with a significantly greater number of proliferating cells (29 ± 8% and 30 ± 8%, respectively). These results indicate that physical modification through the formation of pores on the fiber surface alleviates diffusion limitation of composite spheroids, playing a dominant role over chemical modification.

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Biomimetic Biphasic Electrospun Scaffold for Anterior Cruciate Ligament Tissue Engineering

Cited by 12 publications

References 34 publications

Advancements in scaffold for treating ligament injuries; in vitro evaluation

Advancements in scaffold for treating ligament injuries; in vitro evaluation

Topographic Orientation of Scaffolds for Tissue Regeneration: Recent Advances in Biomaterial Design and Applications

Composite Multicellular Spheroids Containing Fibers with Pores and Epigallocatechin Gallate (EGCG) Coating on the Surface for Enhanced Proliferation of Stem Cells

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