Biomimetic, biodegradable, and osteoinductive Microgels with open porous structure and excellent injectability for construction of microtissues for bone tissue engineering

Xia, Pengfei; Zhang, Kunxi; Yan, Shifeng; Li, Guifei; Yin, Jingbo

doi:10.1016/j.cej.2021.128714

Cited by 16 publications

(8 citation statements)

References 33 publications

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“…EDS analysis showed that C, N, O, Ca, and P were evenly distributed on Ser100-HAP ( Figure 1C ). The molar ratio of Ca to P was 1.60, which is close to the 1.67 value of natural HAP ( Xia et al, 2021 ).…”

Section: Resultssupporting

confidence: 76%

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Ming

Rao

et al. 2022

Front. Bioeng. Biotechnol.

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The guided tissue regeneration (GTR) technique is a promising treatment for periodontal tissue defects. GTR membranes build a mechanical barrier to control the ingrowth of the gingival epithelium and provide appropriate space for the regeneration of periodontal tissues, particularly alveolar bone. However, the existing GTR membranes only serve as barriers and lack the biological activity to induce alveolar bone regeneration. In this study, sericin-hydroxyapatite (Ser-HAP) composite nanomaterials were fabricated using a biomimetic mineralization method with sericin as an organic template. The mineralized Ser-HAP showed excellent biocompatibility and promoted the osteogenic differentiation of human periodontal membrane stem cells (hPDLSCs). Ser-HAP was combined with PVA using the freeze/thaw method to form PVA/Ser-HAP membranes. Further studies confirmed that PVA/Ser-HAP membranes do not affect the viability of hPDLSCs. Moreover, alkaline phosphatase (ALP) staining, alizarin red staining (ARS), and RT-qPCR detection revealed that PVA/Ser-HAP membranes induce the osteogenic differentiation of hPDLSCs by activating the expression of osteoblast-related genes, including ALP, Runx2, OCN, and OPN. The unique GTR membrane based on Ser-HAP induces the differentiation of hPDLSCs into osteoblasts without additional inducers, demonstrating the excellent potential for periodontal regeneration therapy.

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Section: Resultssupporting

confidence: 76%

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Ming

Rao

et al. 2022

Front. Bioeng. Biotechnol.

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“…Nevertheless, injectable microgels cannot meet the growing demand for bone repair on its own. Thus, they are always blended with cells, drugs, genes, factors, and other functional nanoparticles 49–51 …”

Section: Resultsmentioning

confidence: 99%

“…Thus, they are always blended with cells, drugs, genes, factors, and other functional nanoparticles. [49][50][51] Fe 3 O 4 nanoparticles, which have received clinical approval from the Food and Drug Administration, have attracted attention in bone tissue engineering due to their superior magnetic properties and good biocompatibility. 52,53 The magnetic Fe 3 O 4 nanoparticles can construct an endogenous magnetic microenvironment to mediate the osteogenic differentiation of stem cells via magnetic stimuli because of the diamagnetism of cell membranes.…”

Section: Biocompatibility Of the Hydrogel In Vivomentioning

confidence: 99%

Fe₃O₄‐nanoparticle‐functionalized 3D‐printed injectable microgel

Wei,

Zheng,

Zhu

et al. 2023

J of Applied Polymer Sci

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Magnetic Fe3O4 nanoparticles have attached attention in bone tissue engineering because of their superior magnetism and great biocompatibility. However, some disadvantages such as the potential risk of agglomeration impair their applications. Here, we proposed a hybrid magnetic nanocomposite microgel by the integration of Fe3O4 nanoparticles and digital lighting processing (DLP) three‐dimensional (3D) printing technology. The 3D‐printed microgels could be precisely customized by printing the mixture of gelatin methacryloyl (GelMA) solution and polydopamine‐coated Fe3O4 nanoparticles, in which polydopamine decoration improved the hydrophilicity and distribution of the incorporated Fe3O4. The degradable microgels could be injected through a 22‐G needle while retaining their original shape after injection. Interestingly, the addition of Fe3O4 nanoparticles into GelMA solution displayed improved printing accuracy. Moreover, these magnetic microgels were biocompatible in vitro and in vivo. After induction within osteogenic medium, addition of nanoparticles upregulated the osteogenic gene expression of rat bone mesenchymal stem cells (BMSCs). In a word, this work provides a magnetic microplatform, which shows great potential in bone tissue engineering.

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“…The cell proliferation on the microspheres can promote cell-cell interaction, contributing to bone regeneration. [36] Herein, improved cell adhesion might be attributed to the uniform distribution of MgP particles on the surface of SilMA microspheres, which renders the surface considerably rough. Additionally, the nanostructure of MgP spheres facilitated the interaction between cells and microspheres.…”

Section: Tube Formation Cell Migration and Adhesion Assaysmentioning

confidence: 93%

In Situ Enzymatic Reaction Generates Magnesium‐Based Mineralized Microspheres with Superior Bioactivity for Enhanced Bone Regeneration

Cai¹,

Liu

Hu³

et al. 2023

Adv Healthcare Materials

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Bone is a naturally mineralized tissue with a remarkable hierarchical structure, and the treatment of bone defects remains challenging. Microspheres with facile features of controllable size, diverse morphologies, and specific functions display amazing potentials for bone regeneration. Herein, inspired by natural biomineralization, a novel enzyme‐catalyzed reaction is reported to prepare magnesium‐based mineralized microspheres. First, silk fibroin methacryloyl (SilMA) microspheres are prepared using a combination of microfluidics and photo‐crosslinking. Then, the alkaline phosphatase (ALP)‐catalyzed hydrolysis of adenosine triphosphate (ATP) is successfully used to induce the formation of spherical magnesium phosphate (MgP) in the SilMA microspheres. These SilMA@MgP microspheres display uniform size, rough surface structure, good degradability, and sustained Mg2+ release properties. Moreover, the in vitro studies demonstrate the high bioactivities of SilMA@MgP microspehres in promoting the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Transcriptomic analysis shows that the osteoinductivity of SilMA@MgP microspheres may be related to the activation of the PI3K/Akt signaling pathway. Finally, the bone regeneration enhancement units (BREUs) are designed and constructed by inoculating BMSCs onto SilMA@MgP microspheres. In summary, this study demonstrates a new biomineralization strategy for designing biomimetic bone repair materials with defined structures and combination functions.

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Biomimetic, biodegradable, and osteoinductive Microgels with open porous structure and excellent injectability for construction of microtissues for bone tissue engineering

Cited by 16 publications

References 33 publications

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Fe₃O₄‐nanoparticle‐functionalized 3D‐printed injectable microgel

In Situ Enzymatic Reaction Generates Magnesium‐Based Mineralized Microspheres with Superior Bioactivity for Enhanced Bone Regeneration

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Biomimetic, biodegradable, and osteoinductive Microgels with open porous structure and excellent injectability for construction of microtissues for bone tissue engineering

Cited by 16 publications

References 33 publications

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Fe3O4‐nanoparticle‐functionalized 3D‐printed injectable microgel

In Situ Enzymatic Reaction Generates Magnesium‐Based Mineralized Microspheres with Superior Bioactivity for Enhanced Bone Regeneration

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Product

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Fe₃O₄‐nanoparticle‐functionalized 3D‐printed injectable microgel