Biomimetic acellular detoxified glutaraldehyde cross-linked bovine pericardium for tissue engineering

Mathapati, Santosh; Bishi, Dillip Kumar; Guhathakurta, Soma; Cherian, Kotturathu Mammen; Venugopal, Jayarama Reddy; Ramakrishna, Seeram; Verma, Rama Shanker

doi:10.1016/j.msec.2012.12.062

Cited by 45 publications

(29 citation statements)

References 45 publications

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“…The recent approach of "Hybrid Tissue Engineering (HTE)"combines synthetic polymers with natural ECM that maintains a balance between the rate of scaffold degradation and the generation of new ECM to match the features of the native valvular tissue [16,18]. Considering the advantages of HTE, we intended to improve the biomechanical properties of bovine pericardium (BP) which is cheap, durable, easily available off the shelf and currently used as a template for fabrication of commercially available BVs [19]. DBPECM is less immunogenic when compared to BP and retains the bio-matrix components such as Materials Science and Engineering C 51 (2015) 263-273 collagen, elastin and GAG but has weak mechanical properties which makes it unsuitable for tissue engineering of heart valve (TEHV) [20,21].…”

Section: Introductionmentioning

confidence: 99%

Engineering of a polymer layered bio-hybrid heart valve scaffold

Jahnavi

Kumary

Bhuvaneshwar

et al. 2015

Materials Science and Engineering: C

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Section: Introductionmentioning

confidence: 99%

Engineering of a polymer layered bio-hybrid heart valve scaffold

Jahnavi

Kumary

Bhuvaneshwar

et al. 2015

Materials Science and Engineering: C

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“…El pericardio bovino usado en esta investigación no fue sometido a tratamiento alguno, a diferencia del utilizado por Testini et al (2014), que junto a otros investigadores indican que el procesamiento del pericardio bovino con glutaraldehído sirve para evitar antígenos celulares, iones de calcio en la circulación y fosfolípidos presentes en el tejido nativo, los cuales son predominantemente responsables de la calcificación, degeneración y falla de los tejidos implantados como un microambiente natural para la migración de células progenitoras (Mathapati et al, 2013). Sin embargo, los exámenes histopatológicos realizados en los dos equinos no evidenciaron calcificación ni degeneración del colágeno, ni mucho menos inviabilidad (figuras 4-5).…”

Section: Discussionunclassified

Evaluación anatómica e histopatológica de la anastomosis yeyunal equina revestida con xenoinjerto de pericardio bovino

G.¹,

C.²,

Chavera³

et al. 2018

Rev. investig. vet. Perú

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Se hizo la evaluación del xenoinjerto de pericardio bovino no tratado como biomaterial para el revestimiento de la anastomosis de intestino delgado en equinos. Se practicó la cirugía a siete equinos con edades entre 3 y 10 años. Se seleccionaron al azar a dos de ellos, haciendo una laparatomía a uno de ellos a los 15 días y al otro a los 100 días del posoperatorio. En los estudios macroscópicos e histopatológicos de la zona cicatricial de la anastomosis término-terminal se evidenció que el xenoinjerto no se contrae ni se desplaza de la línea de sutura, garantizando el proceso de remodelación cicatricial y evitando complicaciones como peritonitis, estenosis, abscesos, fugas anastomóticas y adherencias. Se concluye que el xenoinjerto de pericardio bovino no tratado es viable y efectivo, como revestimiento de la enterectomía yeyunal; demostrando buena adaptabilidad como microambiente natural para células progenitoras encargadas de la remodelación cicatricial, aunque conlleva a una moderada flogosis y fibrosis que después del periodo de convalecencia desaparecen progresivamente, sin observarse calcificación ni degeneración del colágeno.

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“…We previously demonstrated that the stepwise use of SDS and tritonX-100 produced good decellularization, and a high concentration of SDS could cause structural alteration by compromising collagen integrity. 12,13 Using α-galactosidase in the decellularization process decreases the immune response and prolongs durability of a xenograft. Despite our earlier reports on α-galactosidase treatment, [14][15][16] we were unable to use α-galactosidase in the comprehensive anticalcification procedure because of low cost-effectiveness.…”

Section: Discussionmentioning

confidence: 99%

Early Results of Novel Bovine Pericardial Patch Using Comprehensive Anticalcification Procedure in a Swine Model

Choi

Kim

Kim³

et al. 2016

ASAIO Journal

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We evaluated the short-term safety and effectiveness of our comprehensive anticalcification procedure in swine model. Our comprehensive anticalcification procedure consisted of four steps, including decellularization with sodium dodecyl sulfate and tritonX-100, space filler treatment with polyethylene glycol (PEG), glutaraldehyde cross-linking with organic solvent, and detoxification with glycine. We simultaneously implanted both the commercially available bovine pericardial patch (Supple Peri-Guard) and novel bovine pericardial patch processed by the comprehensive anticalcification procedure into the main pulmonary artery in seven pigs. Every pig underwent a cardiac angiography and was killed on the postoperative day 28. The extracted patches were stained with hematoxylin and eosin. All pigs survived for 4 weeks without any complication. Cardiac angiography showed the absence of leakage and structural problem. Neointimas were formed evenly without intimal hyperplasia. There were no significant differences in the degree of inflammation, necrosis, and calcification between the novel and commercially available patch (p = 0.450, p = 0.317, p = 0.999). Novel bovine pericardial patch using comprehensive anticalcification procedure was similar to existing cardiovascular patch in early surgical results in a swine model. The comprehensive anticalcification procedure could facilitate appropriate bioprosthetic properties of the bovine pericardium.

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Biomimetic acellular detoxified glutaraldehyde cross-linked bovine pericardium for tissue engineering

Cited by 45 publications

References 45 publications

Engineering of a polymer layered bio-hybrid heart valve scaffold

Engineering of a polymer layered bio-hybrid heart valve scaffold

Evaluación anatómica e histopatológica de la anastomosis yeyunal equina revestida con xenoinjerto de pericardio bovino

Early Results of Novel Bovine Pericardial Patch Using Comprehensive Anticalcification Procedure in a Swine Model

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