Biomimetic 3D-printed neurovascular phantoms for near-infrared fluorescence imaging

Liu, Yi; Ghassemi, Pejhman; Depkon, Andrew; Iacono, Maria Ida; Lin, Jonathan; Mendoza, Gonzalo; Wang, Jianting; Tang, Qinggong; Chen, Yu; Pfefer, T. Joshua

doi:10.1364/boe.9.002810

Cited by 35 publications

(37 citation statements)

References 39 publications

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“…The introduction of multi-material 3D printing may ease the development of anatomically relevant phantoms, since the phantoms can be built up layer by layer allowing complex structures to be embedded. Inspiration may be taken from phantoms designed for x-ray imaging [40,41] and fluorescent brain imaging [42]. However, due to limited available 3D printing materials, it is not possible yet to print PA phantoms with both optical and acoustic properties matching the body's soft tissues.…”

Section: Photoacoustic Phantomsmentioning

confidence: 99%

Semi-anthropomorphic photoacoustic breast phantom

Dantuma¹,

Dommelen²,

Manohar³

2019

Biomed. Opt. Express

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Imaging parameters of photoacoustic breast imaging systems such as the spatial resolution and imaging depth are often characterized with phantoms. These objects usually contain simple structures in homogeneous media such as absorbing wires or spherical objects in scattering gels. While these kinds of basic phantoms are uncluttered and useful, they do not challenge the system as much as a breast does, and can thereby overestimate the system's performance. The female breast is a complex collection of tissue types, and the acoustic and optical attenuation of these tissues limit the imaging depth, the resolution and the ability to extract quantitative information. For testing and challenging photoacoustic breast imaging systems to the full extent before moving to in vivo studies, a complex breast phantom which simulates the breast's most prevalent tissues is required. In this work we present the first three dimensional multi-layered semi-anthropomorphic photoacoustic breast phantom. The phantom aims to simulate skin, fat, fibroglandular tissue and blood vessels. The latter three are made from custom polyvinyl chloride plastisol (PVCP) formulations and are appropriately doped with additives to obtain tissue realistic acoustic and optical properties. Two tumors are embedded, which are modeled as clusters of small blood vessels. The PVCP materials are surrounded by a silicon layer mimicking the skin. The tissue mimicking materials were cast into the shapes and sizes expected in the breast using 3D-printed moulds developed from a magnetic resonance imaging segmented numerical breast model. The various structures and layers were assembled to obtain a realistic breast morphology. We demonstrate the phantom's appearance in both ultrasound imaging as photoacoustic tomography and make a comparison with a photoacoustic image of a real breast. A good correspondence is observed, which confirms the phantom's usefulness.

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Section: Photoacoustic Phantomsmentioning

confidence: 99%

Semi-anthropomorphic photoacoustic breast phantom

Dantuma¹,

Dommelen²,

Manohar³

2019

Biomed. Opt. Express

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“…Identifying a suitable channel size involved printing a sample with a range of different channel diameters and using micro-CT imaging to identify the smallest channels that were consistently patent. We previously found that to ensure patency, diameters of approximately 0.8-1 mm were required in biomimetic 3D curved channels [32,33]. This limitation highlights the need for printer quality testing of samples over a range of channel diameters.…”

Section: Evaluation Of Phantom Morphology and Optical Propertiesmentioning

confidence: 99%

“…While we are not aware of any subsequent studies implementing this type of high-density channel-array approach for NIRS, it may now be possible to generate similar phantoms with less effort and greater flexibility using 3D printing technology. Our lab has pioneered the fabrication of 3D-printed biophotonic phantoms [31], developing models with both idealized and biomimetic vascular geometries for evaluation of hyperspectral and fluorescence imaging systems [31][32][33]. Other groups have also shown success in developing 3D-printed phantoms for different biophotonic applications [34,35].…”

Section: Introductionmentioning

confidence: 99%

Cerebral oximetry performance testing with a 3D-printed vascular array phantom

Afshari

Ghassemi

Lin

et al. 2019

Biomed. Opt. Express

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Cerebral oximetry based on near-infrared spectroscopy represents a unique noninvasive tool for real-time surgical monitoring, yet studies have shown a significant discrepancy in accuracy among commercial systems. Towards the establishment of a standardized method for performance testing, we have studied a solid phantom approachbased on a 3D-printed cerebrovascular module (CVM) incorporating an array of 148 cylindrical channels-that has several advantages over liquid phantoms. Development and characterization of a CVM prototype are described, including high-resolution imaging and spectrophotometry measurements. The CVM was filled with whole bovine blood tuned over an oxygen saturation range of 30-90% and molded-silicone layers simulating extracerebral tissues were used to evaluate penetration depth. Saturation measurement accuracy was assessed in two commercially-available clinical cerebral oximeters. For one oximeter, both neonatal and pediatric sensors showed a high degree of precision, whereas accuracy was strongly dependent on saturation level and extracerebral geometry. The second oximeter showed worse precision, yet greater robustness to variations in extracerebral layers. These results indicate that 3D-printed channel array phantoms represent a promising new approach for standardized testing of clinical oximeters.

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“…Phantoms have become increasingly capable of modeling not only the structural characteristics of human anatomy but also the functional attributes. Many phantoms are trending toward having functional attributes and various studies have begun to develop functional phantoms [34][35][36][37][38] with vascular anatomy.…”

Section: Future Of Phantom Designmentioning

confidence: 99%

How to design and construct a 3D-printed human head phantom

2019

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In this paper, we will provide a methodology for head phantom development based on in vivo imaging data attained utilizing MRI. The anthropomorphic phantom can be designed to mimic human anatomy. In medical physics, phantoms are physical and/or numerical tools created to represent specified characteristics of human anatomy [1-5]. Phantoms are a less expensive approach for testing and validation of a variety of applications, such as communication or medical diagnostic imaging tools [1,5-7]. Phantoms offer the capability to perform safety testing of diagnostic imaging tools and potentially minimize harmful exposure to a human. A variety of simple commercial [8] and geometric [3,5] phantoms have been used in various medical applications. The development of anthropomorphic phantoms dates back to the 1960s [9,10]. Additive manufacturing, referred to as 3D printing, has become an increasingly useful tool to develop these complex and anatomically accurate phantoms [11]. In a recent review [12], *

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Biomimetic 3D-printed neurovascular phantoms for near-infrared fluorescence imaging

Cited by 35 publications

References 39 publications

Semi-anthropomorphic photoacoustic breast phantom

Semi-anthropomorphic photoacoustic breast phantom

Cerebral oximetry performance testing with a 3D-printed vascular array phantom

How to design and construct a 3D-printed human head phantom

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