Biomimetic 2D layered double hydroxide nanocomposites for hyperthermia-facilitated homologous targeting cancer photo-chemotherapy

Abstract

Background Multi-modal therapy has attracted increasing attention as it provides enhanced effectiveness and potential stimulation of the immune community. However, low accumulation at the tumor sites and quick immune clearance of the anti-tumor agents are still insurmountable challenges. Hypothetically, cancer cell membrane (CCM) can homologously target the tumor whereas multi-modal therapy can complement the disadvantages of singular therapies. Meanwhile, moderate hyperthermia induced by photothermal therapy can boost the cellular uptake of therapeutic agents by cancer cells. Results CCM-cloaked indocyanine green (ICG)-incorporated and abraxane (PTX-BSA)-loaded layered double hydroxide (LDH) nanosheets (LIPC NSs) were fabricated for target efficient photo-chemotherapy of colorectal carcinoma (CRC). The CCM-cloaked LDH delivery system showed efficient homologous targeting and cytotoxicity, which was further enhanced under laser irradiation to synergize CRC apoptosis. On the other hand, CCM-cloaking remarkably reduced the uptake of LDH NSs by HEK 293T cells and macrophages, implying mitigation of the side effects and the immune clearance, respectively. In vivo data further exhibited that LIPC NSs enhanced the drug accumulation in tumor tissues and significantly retarded tumor progression under laser irradiation at very low therapeutic doses (1.2 and 0.6 mg/kg of ICG and PTX-BSA), without observed side effects on other organs. Conclusions This research has demonstrated that targeting delivery efficiency and immune-escaping ability of LIPC NSs are tremendously enhanced by CCM cloaking for efficient tumor accumulation and in situ generated hyperthermia boosts the uptake of LIPC NSs by cancer cells, a potential effective way to improve the multi-modal cancer therapy. Graphical Abstract

Engineering goat milk-derived extracellular vesicles for multiple bioimaging-guided and photothermal-enhanced therapy of colon cancer

“…ICG is a cyanine compound that displays fluorescence properties in the near-infrared region. 28 It has been used for numerous indications, but it is mainly used for tumor diagnosis and treatments. 12 In this study, bioinformatics analysis was performed to display key genes, important biological processes, and signaling pathways of ICG-mediated PTT.…”

Layered double hydroxide-based nanomaterials for biomedical applications

“…13a). 324 The obtained CCM-LDH delivery system enhanced the drug accumulation in tumor tissues thanks to the homologous targeting ability of CCM, and significantly inhibited tumor growth under 808 nm laser irradiation due to the synergistic effect of ICG-induced heat and abraxane-mediated chemotherapy. The heat generated by PTT further accelerated the fluidity of cancer cells, thereby promoting cellular uptake of therapeutic agents.…”

“…Another paradigm was illustrated by Wang et al , who developed CCM-cloaked MgAl-LDH nanomedicine by co-loading ICG and abraxane. 324 The obtained CCM-LDH delivery system enhanced the drug accumulation in tumor tissues thanks to the homologous targeting ability of CCM, and significantly inhibited tumor growth due to the synergistic PTT/chemotherapy.…”

“Trojan horse” nanoparticle-delivered cancer cell membrane vaccines to enhance cancer immunotherapy by overcoming immune-escape

“…The CT26 cell membrane was extracted using a repeated freeze–thaw–sonication method, as in our previous study. 14 Cell membrane encapsulation was conducted by adding 500 μL of CCM (1 mg mL −1 ) suspension into the CpG-loaded LDH solution described above and shaking overnight at 4 °C. To achieve the optimized CCM amount on the LDH nano-vaccine, 500, 200, 100, 50, and 20 μL of CCM (1 mg mL −1 ) were added into 1 mg of LDH solution.…”